[A preliminary evaluation and discussion on the value of the medical content of book 'Zhu Bing â ', a medical bamboo compilation excavated from a Han dynasty tomb in Laoguanshan].

The compilation of medical bamboo slips, which are excavated from a Han dynasty tomb in Laoguanshan(Mount. Laoguan), has two manuscripts: 'Zhu Bing Ⅰ(cases study Ⅰ)'and 'Zhu Bing Ⅱ(cases study Ⅱ)'. 'Zhu Bing Ⅰ'mainly discussed the pathogenic factor "wind" and "wind diseases" . It also analyzed the features of pathogenic factor "wind" . It classified the "wind diseases" into 17 kinds from 4 point views, and documented the symptoms of 17 kinds of "wind disease" . It was first time that its most ideas were documented in traditional Chinese medicine literatures.

[A preliminary discussion on the contents and value of All Diseases(1), the medical bamboo slips unearthed from Han tomb in Laoguanshan of Chengdu].

Among the medical bamboo slips unearthed from Han tomb in Laoguanshan of Chengdu, the Zhu bing (All Diseases) is a monograph to discuss the characteristics of signs and symptoms. Based on the differences of writing styles, diseases involved, expounding methods and writing rules, the book is divided, by the research team, into 2 parts: All Diseases(1) and All Diseases(2). All Diseases(1) includes over 130 slips, 2 000 characters with totally more than 100 disease names, containing multiple clinical disciplines. The elaborated classification, varied naming methods, grasping the symptom characteristics guided by the four diagnostic approaches, paying attention to the comparison of similar diseases, and dealing with the prognosis and healthcare in this part reflect the holism of correspondence between human body and natural environment, and syndrome differentiation thought of combining disease with symptoms and signs, revealing its academic significance.

[A preliminary discussion on the contents and value of Shi er mai (Twelve Channels) and Bie mai (Allo-channels), the medical bamboo slips unearthed from Han tomb in Laoguanshan of Chengdu].

The 52 medical bamboo slips entitled Si er ma (Twelve Channels) and Bie mai (Allo-channels) , unearthed from Han tomb in Laoguanshan, are the exclusive medical books concerning channels. Shi er mai is the first work bearing the term " heart dominating channel" , and the running pathways and manifestations of 12 channels. This book could be the major original sources of the 12-channel theories described in the Chapter of Jing mai (Channels) of Ling shu (Miraculous Pivot) . While Bie mai suggests the coexistence of the multi-channel systems at the time when the book was compiled and, together with the 12 "channel" , and 3 "branched collaterals" , these 2 books represent the original condition before the establishment of the mode of channel circulation in Ling shu, and is significant to explore the nature and origin of channel.

[A preliminary evaluation and discussion on the significance of the medical bamboo slips Ci shu (Needling Methods) unearthed from a Han tomb in the Mount. Laoguan, Chengdu].

The compilation of medical bamboo slips, Ci shu(Needling Methods), which was unearthed from a Han tomb in Mt. Laoguan, is a monograph dealing exclusively with the principles of clinical acupuncture manipulations with 40 acupuncture prescriptions, being the earliest unearthed work with documented standard methods of acupuncture manipulations and acupuncture prescriptions in China. The chapter Zhen fang (Acupuncture Prescriptions) is the earliest summary of standardized acupoint prescriptions up to now in China, which is of great significance to clinical practice directly derived from ancient clinical performance of acupuncture. The chapter Zhen fang of the book Ci shu is also one of the earliest ancient clinical reports archiving the acupoint. This may provide invaluable perspectives to the study of the conceptualization, origination, development, formation of theoretical system, and clinical application of acupoints.

[Discussion on the species and naming of medical bamboo slips unearthed from Han tomb in Laoguanshan].

920 pieces of bamboo slip, including broken bamboo slips, found in Laoguanshan Han tomb, are placed in two locations. In one of the location, 736 pieces, except 20 pieces of " chi jian" , of medical bamboo slips are stored. The Chengdu Municipal Institute of Archaeology and Cultural Relics Protection Center of Jingzhou preliminary sorted out these pieces and tentatively give them the names: Wu se mai zang lun, Bi xi yi lun, Mai si hou, Liu shi bing fang, Bing yuan lun, Zhu bing zheng hou, Jing mai shu, Gui mai shu. Through our comprehensive reading, font comparison, comparison of the contents, and investigation on the texts, it was suggested that the batch of medical slips might be divided into nine kinds of documents, namely, Bi xi zhen fa, Zhen zhi lun, Liu shi bing fang, Zhu bing yi, Zhu bing er, Shi er mai, Bie mai, Ci shu, Ni shun wu se mai zang yan jing shen.

[Expression characteristics of epithelial markers in human embryonic stem cells differentiating into keratinocytes].

OBJECTIVE: To differentiate human embryonic stem cells (hESCs) into keratinocytes (K-hESCs) and analyse the expression characteristics of biomarkers of K-hESCs. METHODS: The hESCs of line H9 were seeded on matrigel in mTeSR1 medium. The hESCs were directly differentiated into keratinocytes in epithelial differentiation medium with bone morphogenetic protein 4, retinoic acid and N2 supplement. The karyotype of K-hESCs was analyzed, comparing the gene expression differences of K-hESCs with human gingival epithelial cells (HGECs), human immortalized oral epithelial cells (HIOECs) and HaCaT by Real-time PCR. Molecular characteristics of the cell differentiation were observed throughout the process by immunocytochemical techniques. RESULTS: H9-hESCs were successfully differentiated into the cells that exhibited characteristics of keratinocytes in epithelial differentiation medium. The karyotype of K-hESCs was 46, XX; and the keratinocyte gene p63 expression in K-hESCs was significantly lower than that in HaCaT (P < 0.05), but there was no significant difference of p63 expression in K-hESCs, comparing with that in HGECs and HIOECs (P > 0.05). CONCLUSION: H9-hESCs could be directly differentiated into K-hESCs. The gene expression of K-hESCs was similar to that of epithelial cells in the early stage of monolayer cells differentiation with high proliferative activity.

Comparison of solvent extraction and microwave extraction for release of dimethyl sulfide from cereals and canola.

Natural levels of dimethyl sulfide (DMS) in newly harvested wheat, barley, paddy, and canola were determined by gas chromatography using a flame photometric detector in sulfur mode. The two methods involved determination of DMS in the headspace of cereal or oilseed samples (1) after extraction with microwaves and (2) after a traditional approach using 25% KBr solution. Quantitative results from each method were similar, and therefore both methods are suitable for the determination of DMS in grains and oilseeds. However, the microwave procedure has several advantages; for example, results are obtained very quickly, and only a small amount of sample is required.

[Study on chemical constituents of Hemistepta lyrata Bunge].

AIM: To separate and identify the chemical constituents of the whole plant of Hemistepta lyrata Bunge. METHODS: Compounds wese separated with chromatography and their chemical strucrures were elucidated with UV, IR, EIMS, FABMS, ESIMS, 1HNMR, 13CNMR HMQC and HMBC spectral methods. RESULTS: Five compounds were isolated from the plant. They were identified as apigenin I, apigenin-7-O-beta-D-lutinoside II, acacetin-7-O-beta-D-lutinoside III, astragalin IV, hemislienoside V. CONCLUSION: These compounds were isolated from this plant for the first time, and compound V is a new one.

[Studies on chemical constituents from Chinese medicinal plant Hemistepta lyrata Bunge].

OBJECTIVE: To separate and identify the chemical constituents of the whole plant. METHOD: The compounds were extracted with solvents, isolated by column chromatography and identified by spectroscopic methods, such as IR, MS and 1H NMR. RESULT: Five compounds were identified as hentriantane I, taraxasteryl acetate II, taraxasterol III, beta-sitosterol IV, and stigmasterol V. CONCLUSION: The compounds III and V were obtained from the plant for the first time.

Fate of (14)C-labeled carbonyl sulfide on grains and grain fractions.

14C-Labeled carbonyl sulfide (COS) was used to measure the amount of sorbed fumigant and alteration products on grains. Wheat, paddy rice, polished rice, mungbean, and safflower were exposed to a 60 mg L(-)(1) of (14)COS for 7 days and then aired for 5 days. Carbonyl sulfide and/or alteration products in sugars, protein, starch, amino acids, protopectines, and hemicelluloses were undetectable. The total uptakes of radioactivity determined after fractionation and also by extraction were in the range of 36-53 ng g(-)(1) COS equiv. The total radioactivity determined by autoradiography was below the detection limit of 70 ng g(-)(1) COS equiv. Radioactivity in the commodities was less than 0.003% of all the radioactivity applied. The amount of retained radiolabel was measured in three ways. First, biochemical fractions such as lipids and amino acids were separated by chromatography, and the activity was determined in each component. Second, commodities were crushed and extracted in aqueous acetone until the maximum amount of radiolabel was extracted. Third, autoradiography was carried out on commodity kernels.