RESUMEN
OBJECTIVE: To analyze the chemical constituents of supercritical carbon dioxide extraction products from Cnidium monieri. METHOD: Four-factor and three-level orthogonal experimental design was used to optimize the SFE conditions as guided by the content of total coumarins in the extract. The chemical constituents were separated and identified by recrystalization. RESULT: Optimum extraction process was established: 25 MPa as extraction pressure, 50 degrees C as extraction temperature, 6.5 MPa as separation pressure and 60 degrees C as separation temperature. CONCLUSION: Changes in extraction pressure, temperature, time, pulverized degree and separation pressure affect the extracting results remarkably. The two kinds of chemical constituents were separated by recrystallization from C. monieri and identified by the methods of UV, IR, MS, NMR.
Asunto(s)
Cnidium/química , Cumarinas/aislamiento & purificación , Furocumarinas/aislamiento & purificación , Plantas Medicinales/química , Cromatografía con Fluido Supercrítico , Cumarinas/química , Frutas/química , Furocumarinas/químicaAsunto(s)
Lactonas/aislamiento & purificación , Lactuca/química , Plantas Medicinales/química , Sesquiterpenos/aislamiento & purificación , Triterpenos/aislamiento & purificación , Anticolesterolemiantes/aislamiento & purificación , Anticolesterolemiantes/farmacología , Antineoplásicos Fitogénicos/aislamiento & purificación , Antineoplásicos Fitogénicos/farmacología , Hipoglucemiantes/aislamiento & purificación , Hipoglucemiantes/farmacología , Lactonas/química , Estructura Molecular , Sesquiterpenos/química , Triterpenos/químicaRESUMEN
AIM: To study the chemical constituents of Hemistepta lyrata. METHODS: To separate compounds with various chromatography technology and to elucidate their structures by chemical and spectral analysis. RESULTS: Two compounds were isolated from Hemistepta lyrata and their structures were determined as hemiceramide (I), hemisterpene ether (II). CONCLUSION: Compounds I and II are new compounds.