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J Med Food ; 13(6): 1402-8, 2010 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-20874245

RESUMEN

The goal of the this study was to evaluate the mutagenic/antimutagenic effects of conventional (BRS133) and transgenic (BRS 245 RR) soybeans (CS and TS, respectively) in vivo using the bone marrow micronucleus (MN) test, histopathological analysis, chromosome aberration test (CAT), and mitotic index (MI) determination. Six-week-old male Swiss mice were fed with pelleted commercial diet mixed with CS or TS at 10% or 20%. Two experimental designs (MN and CAT) were conducted simultaneously with 10 groups each during a 15-day period. Animals were treated with pelleted commercial diet, CS (10% or 20%), or TS (10% or 20%), and on day 14 they also received cyclophosphamide (CP) (50 mg/kg i.p.). The 10% and 20% CS and TS diets did not significantly decrease the frequencies of micronucleated polychromatic erythrocytes in bone marrow induced by CP. However, the CAT indicated that the 10% and 20% CS diets significantly (P < .05) protected nucleated bone marrow cells against chemical-induced mutagenesis and also produced a significant (P < .05) decrease in the total percentage of spontaneous aberrations. Among the treatments with TS, only the 10% TS diet reduced the percentage of total aberrations induced by CP. The results also indicated that the treatment with 20% TS alone significantly (P < .05) decreased the MI, indicating cytotoxic effects related to the treatment. Taken together, our results suggest that, under the tested conditions, TS and CS have antimutagenic properties and are not toxic.


Asunto(s)
Dieta , Alimentos Modificados Genéticamente , Glycine max , Semillas , Animales , Peso Corporal/efectos de los fármacos , Células de la Médula Ósea/efectos de los fármacos , Aberraciones Cromosómicas/inducido químicamente , Productos Agrícolas , Ciclofosfamida/toxicidad , Alimentos Modificados Genéticamente/efectos adversos , Intestino Delgado/citología , Hígado/citología , Masculino , Ratones , Pruebas de Micronúcleos , Índice Mitótico , Mutágenos/toxicidad , Plantas Modificadas Genéticamente , Lesiones Precancerosas/inducido químicamente , Lesiones Precancerosas/prevención & control , Semillas/efectos adversos , Semillas/química , Glycine max/efectos adversos , Glycine max/química , Glycine max/genética , Estómago/citología
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