RESUMEN
Hearing loss is a major public health concern with no pharmaceutical intervention for hearing protection or restoration. Using zebrafish neuromast hair cells, a robust model for mammalian auditory and vestibular hair cells, we identified a urea-thiophene carboxamide, 1 (ORC-001), as protective against aminoglycoside antibiotic (AGA)-induced hair cell death. The 50% protection (HC50) concentration conferred by 1 is 3.2 µM with protection against 200 µM neomycin approaching 100%. Compound 1 was sufficiently safe and drug-like to validate otoprotection in an in vivo rat hearing loss model. We explored the structure-activity relationship (SAR) of this compound series to improve otoprotective potency, improve pharmacokinetic properties and eliminate off-target activity. We present the optimization of 1 to yield 90 (ORC-13661). Compound 90 protects mechanosensory hair cells with HC50 of 120 nM and demonstrates 100% protection in the zebrafish assay and superior physiochemical, pharmacokinetic, and toxicologic properties, as well as complete in vivo protection in rats.
Asunto(s)
Aminoglicósidos/efectos adversos , Pérdida Auditiva/inducido químicamente , Pérdida Auditiva/prevención & control , Seguridad , Tiofenos/química , Tiofenos/farmacología , Urea/química , Administración Oral , Animales , Evaluación Preclínica de Medicamentos , Ratas , Relación Estructura-Actividad , Tiofenos/administración & dosificación , Tiofenos/efectos adversos , Pez CebraRESUMEN
HYPOTHESIS: The zebrafish lateral line can be used to identify small molecules that protect against cisplatin-induced hair cell death. BACKGROUND: Cisplatin is a commonly used chemotherapeutic agent, which causes hearing loss by damaging hair cells of the inner ear. There are currently no FDA-approved pharmacologic strategies for preventing this side effect. The zebrafish lateral line has been used successfully in the past to study hair cell death and protection. METHODS: In this study, we used the zebrafish lateral line to screen a library of 10,000 small molecules for protection against cisplatin-induced hair cell death. Dose-response relationships for identified protectants were determined by quantifying hair cell protection. The effect of each protectant on uptake of a fluorescent cisplatin analog was also quantified. RESULTS: From this screen, we identified 2 compounds exhibiting dose-dependent protection: cisplatin hair cell protectant 1 and 2 (CHCP1 and 2). CHCP1 reduced the uptake of a fluorescent cisplatin analog, suggesting its protective effects may be due to decreased cisplatin uptake. CHCP2 did not affect uptake, which suggests an intracellular mechanism of action. Evaluation of analogs of CHCP2 revealed 3 additional compounds that significantly reduced cisplatin-induced hair cell death, although none exceed the effectiveness or potency of the parent compound. CONCLUSION: The zebrafish lateral line was used to identify 2 small molecules that protected against cisplatin-induced hair cell death.
Asunto(s)
Antineoplásicos/toxicidad , Muerte Celular/efectos de los fármacos , Cisplatino/toxicidad , Células Ciliadas Auditivas/efectos de los fármacos , Pérdida Auditiva/prevención & control , Sistema de la Línea Lateral/efectos de los fármacos , Fármacos Neuroprotectores/farmacología , Animales , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos , Pérdida Auditiva/inducido químicamente , Pez CebraRESUMEN
Zebrafish (Danio rerio) have become a valuable model for investigating the molecular genetics and development of the inner ear in vertebrates. In this study, we employed a prepulse inhibition (PPI) paradigm to assess hearing in larval wild-type (AB) zebrafish during early development at 5-6 days post-fertilization (d.p.f.). We measured the PPI of the acoustic startle response in zebrafish using a 1-dimensional shaker that simulated the particle motion component of sound along the fish's dorsoventral axis. The thresholds to startle-inducing stimuli were determined in 5-6 d.p.f. zebrafish, and their hearing sensitivity was then characterized using the thresholds of prepulse tone stimuli (90-1200 Hz) that inhibited the acoustic startle response to a reliable startle stimulus (820 Hz at 20 dB re. 1 m s(-2)). Hearing thresholds were defined as the minimum prepulse tone level required to significantly reduce the startle response probability compared with the baseline (no-prepulse) condition. Larval zebrafish showed greatest auditory sensitivity from 90 to 310 Hz with corresponding mean thresholds of -19 to -10 dB re. 1 m s(-2), respectively. Hearing thresholds of prepulse tones were considerably lower than previously predicted by startle response assays. The PPI assay was also used to investigate the relative contribution of the lateral line to the detection of acoustic stimuli. After aminoglycoside-induced neuromast hair-cell ablation, we found no difference in PPI thresholds between treated and control fish. We propose that this PPI assay can be used to screen for novel zebrafish hearing mutants and to investigate the ontogeny of hearing in zebrafish and other fishes.
Asunto(s)
Percepción Auditiva/fisiología , Conducta Animal/fisiología , Pruebas Auditivas/métodos , Pez Cebra/fisiología , Estimulación Acústica , Animales , Umbral Auditivo/fisiología , Femenino , Larva/fisiología , Sistema de la Línea Lateral/fisiología , Masculino , Reflejo de Sobresalto/fisiología , Espectrografía del Sonido , Factores de TiempoRESUMEN
Cisplatin, one of the most commonly used anticancer drugs, is known to cause inner ear hair cell damage and hearing loss. Despite much investigation into mechanisms of cisplatin-induced hair cell death, little is known about the mechanism whereby cisplatin is selectively toxic to hair cells. Using hair cells of the zebrafish lateral line, we found that chemical inhibition of mechanotransduction with quinine and EGTA protected against cisplatin-induced hair cell death. Furthermore, we found that the zebrafish mutants mariner (myo7aa) and sputnik (cad23) that lack functional mechanotransduction were resistant to cisplatin-induced hair cell death. Using a fluorescent analog of cisplatin, we found that chemical or genetic inhibition of mechanotransduction prevented its uptake. These findings demonstrate that cisplatin-induced hair cell death is dependent on functional mechanotransduction in the zebrafish lateral line.
Asunto(s)
Antineoplásicos/farmacología , Cisplatino/farmacología , Células Ciliadas Auditivas/efectos de los fármacos , Sistema de la Línea Lateral/citología , Mecanorreceptores/efectos de los fármacos , Animales , Animales Modificados Genéticamente , Calcio/metabolismo , Recuento de Células/métodos , Muerte Celular/efectos de los fármacos , Muerte Celular/genética , Ácido Egtácico/farmacología , Embrión no Mamífero , Femenino , Colorantes Fluorescentes , Proteínas Fluorescentes Verdes/genética , Células Ciliadas Auditivas/metabolismo , Larva , Sistema de la Línea Lateral/efectos de los fármacos , Masculino , Microscopía Fluorescente , Miosina VIIa , Miosinas/metabolismo , Quinina/farmacología , Pez Cebra , Proteínas de Pez Cebra/genéticaRESUMEN
Loss of mechanosensory hair cells in the inner ear accounts for many hearing loss and balance disorders. Several beneficial pharmaceutical drugs cause hair cell death as a side effect. These include aminoglycoside antibiotics, such as neomycin, kanamycin and gentamicin, and several cancer chemotherapy drugs, such as cisplatin. Discovering new compounds that protect mammalian hair cells from toxic insults is experimentally difficult because of the inaccessibility of the inner ear. We used the zebrafish lateral line sensory system as an in vivo screening platform to survey a library of FDA-approved pharmaceuticals for compounds that protect hair cells from neomycin, gentamicin, kanamycin and cisplatin. Ten compounds were identified that provide protection from at least two of the four toxins. The resulting compounds fall into several drug classes, including serotonin and dopamine-modulating drugs, adrenergic receptor ligands, and estrogen receptor modulators. The protective compounds show different effects against the different toxins, supporting the idea that each toxin causes hair cell death by distinct, but partially overlapping, mechanisms. Furthermore, some compounds from the same drug classes had different protective properties, suggesting that they might not prevent hair cell death by their known target mechanisms. Some protective compounds blocked gentamicin uptake into hair cells, suggesting that they may block mechanotransduction or other routes of entry. The protective compounds identified in our screen will provide a starting point for studies in mammals as well as further research discovering the cellular signaling pathways that trigger hair cell death.
Asunto(s)
Aminoglicósidos/antagonistas & inhibidores , Cisplatino/antagonistas & inhibidores , Células Ciliadas Auditivas/efectos de los fármacos , Fármacos Neuroprotectores/farmacología , Aminoglicósidos/toxicidad , Animales , Antibacterianos/antagonistas & inhibidores , Antibacterianos/toxicidad , Antineoplásicos/antagonistas & inhibidores , Antineoplásicos/toxicidad , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Cisplatino/toxicidad , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos , Interacciones Farmacológicas , Gentamicinas/antagonistas & inhibidores , Gentamicinas/toxicidad , Células Ciliadas Auditivas/patología , Humanos , Kanamicina/antagonistas & inhibidores , Kanamicina/toxicidad , Sistema de la Línea Lateral/efectos de los fármacos , Sistema de la Línea Lateral/patología , Mecanotransducción Celular/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Neomicina/antagonistas & inhibidores , Neomicina/toxicidad , Moduladores Selectivos de los Receptores de Estrógeno/farmacología , Pez CebraRESUMEN
Afferent input regulates neuronal dendritic patterning locally and globally through distinct mechanisms. To begin to understand these mechanisms, we differentially manipulate afferent input in vivo and assess effects on dendritic patterning of individual neurons in chicken nucleus laminaris (NL). Dendrites of NL neurons segregate into dorsal and ventral domains, receiving excitatory input from the ipsilateral and contralateral ears, respectively, via nucleus magnocellularis (NM). Blocking action potentials from one ear, by either cochlea removal or temporary treatment with tetrodotoxin (TTX), leads to rapid and significant retraction of affected NL dendrites (dorsal ipsilaterally and ventral contralaterally) within 8 h compared with the other dendrites of the same neurons. The degree of retraction is comparable with that induced by direct deafferentation resulting from transection of NM axons. Importantly, when inner ear activity is allowed to recover from TTX treatments, retracted NL dendrites regrow to their normal length within 48 h. The retraction and growth involve elimination of terminal branches and addition of new branches, respectively. Examination of changes in NL dendrites at 96 h after unilateral cochlea removal, a manipulation that induces cell loss in NM and persistent blockage of afferent excitatory action potentials, reveals a significant correlation between cell death in the ipsilateral NM and the degree of dendritic retraction in NL. These results demonstrate that presynaptic action potentials rapidly and reversibly regulate dendritic patterning of postsynaptic neurons in a compartment specific manner, whereas long-term dendritic maintenance may be regulated in a way that is correlated with the presence of silent presynaptic appositions.
Asunto(s)
Vías Auditivas/fisiología , Tronco Encefálico/citología , Dendritas/fisiología , Células Receptoras Sensoriales/citología , Estimulación Acústica , Análisis de Varianza , Animales , Animales Recién Nacidos , Vías Auditivas/lesiones , Tronco Encefálico/fisiología , Muerte Celular/fisiología , Pollos , Dendritas/efectos de los fármacos , Dextranos/metabolismo , Potenciales Evocados Auditivos del Tronco Encefálico/efectos de los fármacos , Potenciales Evocados Auditivos del Tronco Encefálico/fisiología , Femenino , Imagenología Tridimensional , Técnicas In Vitro , Masculino , Proteínas Asociadas a Microtúbulos/metabolismo , Modelos Neurológicos , Psicoacústica , Privación Sensorial/fisiología , Células Receptoras Sensoriales/efectos de los fármacos , Células Receptoras Sensoriales/fisiología , Bloqueadores de los Canales de Sodio/farmacología , Tetrodotoxina/farmacología , Factores de TiempoRESUMEN
Topographic maps are salient features of neuronal organization in sensory systems. Inhibitory components of neuronal circuitry are often embedded within this organization, making them difficult to isolate experimentally. The auditory system provides opportunities to study the topographic organization of inhibitory long-range projection nuclei, such as the superior olivary nucleus (SON). We analyzed the topographic organization of response features of neurons in the SON of chickens. Quantitative methods were developed to assess and communicate this organization. These analyses led to three main conclusions: 1) sound frequency is linearly arranged from dorsal (low frequencies) to ventral (high frequencies) in SON; 2) this tonotopic organization is less precise than the organization of the excitatory nuclei in the chicken auditory brainstem; and 3) neurons with different response patterns to pure tone stimuli are interspersed throughout the SON and show similar tonotopic organizations. This work provides a predictive model to determine the optimal stimulus frequency for a neuron from its spatial location in the SON.
Asunto(s)
Percepción Auditiva/fisiología , Mapeo Encefálico , Neuronas/fisiología , Núcleo Olivar/fisiología , Estimulación Acústica , Animales , Vías Auditivas/fisiología , Pollos , Electrofisiología , Procesamiento de Imagen Asistido por Computador , Imagenología TridimensionalRESUMEN
The avian nucleus laminaris (NL) is involved in computation of interaural time differences (ITDs) that encode the azimuthal position of a sound source. Neurons in NL are bipolar, with dorsal and ventral dendritic arbors receiving input from separate ears. NL neurons act as coincidence detectors that respond maximally when input from each ear arrives at the two dendritic arbors simultaneously. Computational and physiological studies demonstrated that the sensitivity of NL neurons to coincident inputs is modulated by an inhibitory feedback circuit via the superior olivary nucleus (SON). To understand the mechanism of this modulation, the topography of the projection from SON to NL was mapped, and the morphology of the axon terminals of SON neurons in NL was examined in chickens (Gallus gallus). In vivo injection of AlexaFluor 568 dextran amine into SON demonstrated a coarse topographic projection from SON to NL. Retrogradely labeled neurons in NL were located within the zone of anterogradely labeled terminals, suggesting a reciprocal projection between SON to NL. In vivo extracellular physiological recording further demonstrated that this topography is consistent with tonotopic maps in SON and NL. In addition, three-dimensional reconstruction of single SON axon branches within NL revealed that individual SON neurons innervate a large area of NL and terminate on both dorsal and ventral dendritic arbors of NL neurons. The organization of the projection from SON to NL supports its proposed functions of controlling the overall activity level of NL and enhancing the specificity of frequency mapping and ITD detection.
Asunto(s)
Vías Auditivas/anatomía & histología , Tronco Encefálico/anatomía & histología , Pollos , Núcleo Olivar/anatomía & histología , Estimulación Acústica , Animales , Vías Auditivas/fisiología , Percepción Auditiva/fisiología , Biomarcadores/metabolismo , Mapeo Encefálico , Tronco Encefálico/fisiología , Inhibición Neural/fisiología , Núcleo Olivar/fisiología , Sonido , Factores de TiempoRESUMEN
GABAergic modulation of activity in avian cochlear nucleus neurons has been studied extensively in vitro. However, how this modulation actually influences processing in vivo is not known. We investigated responses of chicken nucleus magnocellularis (NM) neurons to sound while pharmacologically manipulating the inhibitory input from the superior olivary nucleus (SON). SON receives excitatory inputs from nucleus angularis (NA) and nucleus laminaris (NL), and provides GABAergic inputs to NM, NA, NL, and putatively to the contralateral SON. Results from single-unit extracellular recordings from 2 to 4 weeks posthatch chickens show that firing rates of auditory nerve fibers increased monotonically with sound intensity, while that of NM neurons saturated or even decreased at moderate or loud sound levels. Blocking GABAergic input with local application of TTX into the SON induced an increase in firing rate of ipsilateral NM, while that of the contralateral NM decreased at high sound levels. Moreover, local application of bicuculline to NM also increased the firing rate of NM neurons at high sound levels, reduced phase locking, and broadened the frequency-tuning properties of NM neurons. Following application of DNQX, clear evidence of inhibition was observed. Furthermore, the inhibition was tuned to a broader frequency range than the excitatory response areas. We conclude that GABAergic inhibition from SON has at least three physiological influences on the activity of NM neurons: it regulates the firing activity of NM units in a sound-level-dependent manner; it improves phase selectivity; and it sharpens frequency tuning of NM neuronal responses.
Asunto(s)
Potenciales de Acción/fisiología , Núcleo Coclear/citología , Inhibición Neural/fisiología , Neuronas/citología , Ácido gamma-Aminobutírico/metabolismo , Estimulación Acústica/métodos , Potenciales de Acción/efectos de los fármacos , Análisis de Varianza , Animales , Animales Recién Nacidos , Bicuculina/farmacología , Pollos/anatomía & histología , Antagonistas de Aminoácidos Excitadores/farmacología , Lateralidad Funcional , Antagonistas del GABA/farmacología , Inhibición Neural/efectos de los fármacos , Neuronas/clasificación , Neuronas/efectos de los fármacos , Psicoacústica , Quinoxalinas/farmacología , Bloqueadores de los Canales de Sodio/farmacología , Tetrodotoxina/farmacología , Factores de TiempoRESUMEN
In humans, most hearing loss results from death of hair cells, the mechanosensory receptors of the inner ear. Two goals of current hearing research are to protect hair cells from degeneration and to regenerate new hair cells, replacing those that are lost due to aging, disease, or environmental challenges. One limitation of research in the auditory field has been the relative inaccessibility of the mechanosensory systems in the inner ear. Zebrafish possess hair cells in both their inner ear and their lateral line system that are morphologically and functionally similar to human hair cells. The external location of the mechanosensory hair cells in the lateral line and the ease of in vivo labeling and imaging make the zebrafish lateral line a unique system for the study of hair cell toxicity, protection, and regeneration. This review focuses on the lateral line system as a model for understanding loss and protection of mechanosensory hair cells. We discuss chemical screens to identify compounds that induce hair cell loss and others that protect hair cells from known toxins and the potential application of these screens to human medicine.
Asunto(s)
Células Ciliadas Auditivas/efectos de los fármacos , Sistema de la Línea Lateral/efectos de los fármacos , Pez Cebra , Animales , Supervivencia Celular/efectos de los fármacos , Sistemas de Liberación de Medicamentos , Evaluación Preclínica de Medicamentos , Células Ciliadas Auditivas/citología , Humanos , Sistema de la Línea Lateral/citologíaRESUMEN
Several animal models have been used for the study of mechanosensory hair cells and hearing loss. Because of the difficulty of tissue acquisition and large animal size, these traditional models are impractical for high-throughput screening. The zebrafish has emerged as a powerful animal model for screening drugs that cause and prevent hair cell death. The unique characteristics of the zebrafish enable rapid in vivo imaging of hair cells and hair cell death. We have used this model to screen for and identify multiple drugs that protect hair cells from aminoglycoside-induced death. The identification of multiple drugs and drug-like compounds that inhibit multiple hair cell death pathways might enable the development of protective cocktails to achieve complete hair cell protection.
Asunto(s)
Evaluación Preclínica de Medicamentos/métodos , Efectos Colaterales y Reacciones Adversas Relacionados con Medicamentos , Pérdida Auditiva/inducido químicamente , Pérdida Auditiva/tratamiento farmacológico , Sistema de la Línea Lateral/fisiología , Pez Cebra/fisiología , Animales , Línea Celular , Modelos Animales de Enfermedad , Células Ciliadas Auditivas/efectos de los fármacos , Células Ciliadas Auditivas/fisiología , Humanos , Sustancias Protectoras/farmacologíaRESUMEN
The frequency organization of neurons in the forebrain Field L complex (FLC) of adult starlings was investigated to determine the effects of hair cell (HC) destruction in the basal portion of the basilar papilla (BP) and of subsequent HC regeneration. Conventional microelectrode mapping techniques were used in normal starlings and in lesioned starlings either 2 d or 6-10 weeks after aminoglycoside treatment. Histological examination of the BP and recordings of auditory brainstem evoked responses confirmed massive loss of HCs in the basal portion of the BP and hearing losses at frequencies >2 kHz in starlings tested 2 d after aminoglycoside treatment. In these birds, all neurons in the region of the FLC in which characteristic frequencies (CFs) normally increase from 2 to 6 kHz had CF in the range of 2-4 kHz. The significantly elevated thresholds of responses in this region of altered tonotopic organization indicated that they were the residue of prelesion responses and did not reflect CNS plasticity. In the long-term recovery birds, there was histological evidence of substantial HC regeneration. The tonotopic organization of the high-frequency region of the FLC did not differ from that in normal starlings, but the mean threshold at CF in this frequency range was intermediate between the values in the normal and lesioned short-recovery groups. The recovery of normal tonotopicity indicates considerable stability of the topography of neuronal connections in the avian auditory system, but the residual loss of sensitivity suggests deficiencies in high-frequency HC function.
Asunto(s)
Corteza Auditiva/patología , Células Ciliadas Auditivas/fisiología , Regeneración Nerviosa/fisiología , Órgano Espiral/lesiones , Estorninos/fisiología , Estimulación Acústica/métodos , Aminoglicósidos/farmacología , Animales , Vías Auditivas/fisiología , Umbral Auditivo/fisiología , Mapeo Encefálico , Potenciales Evocados Auditivos del Tronco Encefálico/fisiología , Células Ciliadas Auditivas/patología , Kanamicina/toxicidad , Modelos Lineales , Regeneración Nerviosa/efectos de los fármacos , Órgano Espiral/fisiopatología , Inhibidores de la Síntesis de la Proteína/toxicidad , Recuperación de la Función/fisiología , Factores de TiempoRESUMEN
Aminoglycoside antibiotics cause death of sensory hair cells. Research over the past decade has identified several key players in the intracellular cascade. However, the role of the extracellular environment in aminoglycoside ototoxicity has received comparatively little attention. The present study uses the zebrafish lateral line to demonstrate that extracellular calcium and magnesium ions modulate hair cell death from neomycin and gentamicin in vivo, with high levels of either divalent cation providing significant protection. Imaging experiments with fluorescently-tagged gentamicin show that drug uptake is reduced under high calcium conditions. Treating fish with the hair cell transduction blocker amiloride also reduces aminoglycoside uptake, preventing the toxicity, and experiments with variable calcium and amiloride concentrations suggest complementary effects between the two protectants. Elevated magnesium, in contrast, does not appear to significantly attenuate drug uptake, suggesting that the two divalent cations may protect hair cells from aminoglycoside damage through different mechanisms. These results provide additional evidence for calcium- and transduction-dependent aminoglycoside uptake. Divalent cations provided differential protection from neomycin and gentamicin, with high cation concentrations almost completely protecting hair cells from neomycin and acute gentamicin toxicity, but offering reduced protection from continuous (6 h) gentamicin exposure. These experiments lend further support to the hypothesis that aminoglycoside toxicity occurs via multiple pathways in a both a drug and time course-specific manner.
Asunto(s)
Aminoglicósidos/antagonistas & inhibidores , Aminoglicósidos/toxicidad , Cationes Bivalentes/farmacología , Sistema de la Línea Lateral/efectos de los fármacos , Sistema de la Línea Lateral/patología , Mecanorreceptores/efectos de los fármacos , Mecanorreceptores/patología , Animales , Antibacterianos/antagonistas & inhibidores , Antibacterianos/toxicidad , Calcio/farmacología , Muerte Celular/efectos de los fármacos , Gentamicinas/antagonistas & inhibidores , Gentamicinas/toxicidad , Magnesio/farmacología , Neomicina/antagonistas & inhibidores , Neomicina/toxicidad , Pez Cebra/anatomía & histologíaRESUMEN
The hair cells of the larval zebrafish lateral line provide a useful preparation in which to study hair cell death and to screen for genes and small molecules that modulate hair cell toxicity. We recently reported preliminary results from screening a small-molecule library for compounds that inhibit aminoglycoside-induced hair cell death. To potentially reduce the time required for development of drugs and drug combinations that can be clinically useful, we screened a library of 1,040 FDA-approved drugs and bioactive compounds (NINDS Custom Collection II). Seven compounds that protect against neomycin-induced hair cell death were identified. Four of the seven drugs inhibited aminoglycoside uptake, based on Texas-Red-conjugated gentamicin uptake. The activities of two of the remaining three drugs were evaluated using an in vitro adult mouse utricle preparation. One drug, 9-amino-1,2,3,4-tetrahydroacridine (tacrine) demonstrated conserved protective effects in the mouse utricle. These results demonstrate that the zebrafish lateral line can be used to screen successfully for drugs within a library of FDA-approved drugs and bioactives that inhibit hair cell death in the mammalian inner ear and identify tacrine as a promising protective drug for future studies.
Asunto(s)
Evaluación Preclínica de Medicamentos/métodos , Células Ciliadas Auditivas/efectos de los fármacos , Sistema de la Línea Lateral/efectos de los fármacos , Preparaciones Farmacéuticas/administración & dosificación , Sáculo y Utrículo/efectos de los fármacos , Animales , Antibacterianos/administración & dosificación , Antibacterianos/toxicidad , Supervivencia Celular/efectos de los fármacos , Inhibidores de la Colinesterasa/administración & dosificación , Relación Dosis-Respuesta a Droga , Masculino , Mecanotransducción Celular , Ratones , Neomicina/administración & dosificación , Neomicina/toxicidad , Tacrina/administración & dosificación , Estados Unidos , United States Food and Drug Administration , Pez CebraRESUMEN
Inner ear sensory hair cell death is observed in the majority of hearing and balance disorders, affecting the health of more than 600 million people worldwide. While normal aging is the single greatest contributor, exposure to environmental toxins and therapeutic drugs such as aminoglycoside antibiotics and antineoplastic agents are significant contributors. Genetic variation contributes markedly to differences in normal disease progression during aging and in susceptibility to ototoxic agents. Using the lateral line system of larval zebrafish, we developed an in vivo drug toxicity interaction screen to uncover genetic modulators of antibiotic-induced hair cell death and to identify compounds that confer protection. We have identified 5 mutations that modulate aminoglycoside susceptibility. Further characterization and identification of one protective mutant, sentinel (snl), revealed a novel conserved vertebrate gene. A similar screen identified a new class of drug-like small molecules, benzothiophene carboxamides, that prevent aminoglycoside-induced hair cell death in zebrafish and in mammals. Testing for interaction with the sentinel mutation suggests that the gene and compounds may operate in different pathways. The combination of chemical screening with traditional genetic approaches is a new strategy for identifying drugs and drug targets to attenuate hearing and balance disorders.
Asunto(s)
Células Ciliadas Auditivas Internas/citología , Células Ciliadas Auditivas Internas/fisiología , Pez Cebra/anatomía & histología , Pez Cebra/genética , Aminoglicósidos/antagonistas & inhibidores , Aminoglicósidos/toxicidad , Animales , Secuencia de Bases , Muerte Celular/efectos de los fármacos , Muerte Celular/genética , Cisplatino/toxicidad , Codón de Terminación/genética , Cartilla de ADN/genética , ADN Complementario/genética , Evaluación Preclínica de Medicamentos , Epistasis Genética , Células Ciliadas Auditivas Internas/efectos de los fármacos , Pérdida Auditiva/etiología , Pérdida Auditiva/genética , Pérdida Auditiva/prevención & control , Humanos , Ratones , Neomicina/antagonistas & inhibidores , Neomicina/toxicidad , Mutación Puntual , Sáculo y Utrículo/efectos de los fármacos , Sáculo y Utrículo/patología , Tiofenos/química , Tiofenos/farmacología , Pez Cebra/fisiologíaRESUMEN
The zebrafish is a valuable model for studying hair cell development, structure, genetics, and behavior. Zebrafish and other aquatic vertebrates have hair cells on their body surface organized into a sensory system called the lateral line. These hair cells are highly accessible and easily visualized using fluorescent dyes. Morphological and functional similarities to mammalian hair cells of the inner ear make the zebrafish a powerful preparation for studying hair cell toxicity. The ototoxic potential of drugs has historically been uncovered by anecdotal reports that have led to more formal investigation. Currently, no standard screen for ototoxicity exists in drug development. Thus, for the vast majority of Food and Drug Association (FDA)-approved drugs, the ototoxic potential remains unknown. In this study, we used 5-day-old zebrafish larvae to screen a library of 1,040 FDA-approved drugs and bioactives (NINDS Custom Collection II) for ototoxic effects in hair cells of the lateral line. Hair cell nuclei were selectively labeled using a fluorescent vital dye. For the initial screen, fish were exposed to drugs from the library at a 100-muM concentration for 1 h in 96-well tissue culture plates. Hair cell viability was assessed in vivo using fluorescence microscopy. One thousand forty drugs were rapidly screened for ototoxic effects. Seven known ototoxic drugs included in the library, including neomycin and cisplatin, were positively identified using these methods, as proof of concept. Fourteen compounds without previously known ototoxicity were discovered to be selectively toxic to hair cells. Dose-response curves for all 21 ototoxic compounds were determined by quantifying hair cell survival as a function of drug concentration. Dose-response relationships in the mammalian inner ear for two of the compounds without known ototoxicity, pentamidine isethionate and propantheline bromide, were then examined using in vitro preparations of the adult mouse utricle. Significant dose-dependent hair cell loss in the mouse utricle was demonstrated for both compounds. This study represents an important step in validating the use of the zebrafish lateral line as a screening tool for the identification of potentially ototoxic drugs.