RESUMEN
The methanolic crude extract from the roots of Jacquinia flammea showed moderate antifungal activity against dermatophytes and very strong antifungal activity against Colletotrichum gloeosporioides. The bioassay-guided purification of the extract, using a combination of vacuum-liquid chromatography and high performance liquid chromatography, allowed the identification of sakurasosaponin as the main metabolite responsible for the antifungal activity.
Asunto(s)
Antifúngicos/farmacología , Extractos Vegetales/farmacología , Raíces de Plantas/química , Primulaceae/química , Saponinas/farmacología , Antifúngicos/análisis , Antifúngicos/química , Arthrodermataceae/efectos de los fármacos , Cromatografía Liquida , Colletotrichum/efectos de los fármacos , Metanol , Estructura Molecular , Extractos Vegetales/análisis , Extractos Vegetales/química , Saponinas/análisis , Saponinas/químicaRESUMEN
Extracts from leaves, stems, and roots of twelve plants used commonly in Yucatecan traditional medicine were evaluated in the DNA-methyl green assay. Twenty one extracts showed DNA-interacting activity, and nine of them, belonging to five plant species, presented a displacement activity of 5% or higher. The highest activity (17.6%) was detected in the leaf extract of Heliotropium angiospermum.
Asunto(s)
ADN/efectos de los fármacos , Medicina Tradicional , Extractos Vegetales/farmacología , Plantas Medicinales , Evaluación Preclínica de Medicamentos , Etidio/farmacología , Verde de Metilo , México , Modelos Biológicos , Farmacognosia , Extractos Vegetales/uso terapéutico , Estructuras de las PlantasRESUMEN
Bioactivity of extracts from leaves, stems and roots of twelve plants commonly used in Yucatecan traditional medicine were evaluated in four bioassays. Crude extracts from ten plants showed significant activity in the inhibition of bleaching of beta-carotene assay, while thirteen extracts showed activity in the reduction of 2, 2- diphenyl-1-picrylhydrazyl radical (DPPH) assay. In the antimicrobial bioassay, the major activity was presented by the root extract of Jatropha gaumeri and in the beta-glucosidase inhibition activity assay the strongest activity was observed in the stem and root extracts of Solanum hirtum.