RESUMEN
Preliminary screening of antiviral AIDS drugs has been carried out using three different in vitro assay systems. Among 96 samples of different origin tested, two were shown to inhibit the growth of HIV in vitro. One of the positive samples (plant origin) has hopeful signs, as the ranges of effective doses are wider than those of most of positive samples which had been found by us.
Asunto(s)
Síndrome de Inmunodeficiencia Adquirida/tratamiento farmacológico , Fármacos Anti-VIH/farmacología , Evaluación Preclínica de Medicamentos/métodosRESUMEN
The crude water extract (NS-1) from a seaweed (Digenea simplex C. Agardh Rhodomelaceae) exhibited anti-human immunodeficiency virus (HIV)-1 activity in vitro. The inhibitory effect of the extract on the cytopathic activity of HIV-1 and it's antigen production was examined using a microplate method, immunofluorescent assay, and an HIV antigen detection kit (Abbott). NS-1 inhibited both the cytopathic effect of HIV-1 to MT-4 cells and the giant cell formation of Molt-4 cells infected with HIV-1.
Asunto(s)
Antivirales/farmacología , Efecto Citopatogénico Viral/efectos de los fármacos , Antígenos VIH/biosíntesis , VIH-1/efectos de los fármacos , Extractos Vegetales/farmacología , Antivirales/química , Línea Celular , Fenómenos Químicos , Química Física , Técnica del Anticuerpo Fluorescente Indirecta , VIH-1/inmunología , Humanos , Peso Molecular , Extractos Vegetales/química , Linfocitos T/inmunología , Linfocitos T/virologíaRESUMEN
Preliminary screening of antiviral AIDS drugs has been carried out using three different in vitro assay systems. Among 191 samples tested, seven were found to inhibit the growth of HIV in vitro. Four of seven have hopeful signs, as the ranges of effective doses of the samples are wide.