RESUMEN
Plants constitute a valuable source of natural antioxidants such as polyphenols and are responsible for exhibiting many biologically significant functions. Ruta species including Ruta chalepensis L. and Ruta graveolens L. are widespread species in Algeria and are used as medicinal plants to treat various diseases; however, so far, most of the conducted studies focused on analyzing alkaloids and essential oils mostly on R. chalepensis. The aim of the present research is to investigate the phenolic profile of the aerial parts of Ruta graveolens L. from Algeria and assess its inâ vitro antioxidant, anti-inflammatory and antimicrobial properties. The total polyphenols and flavonoids were assessed using colorimetric methods, and the individual polyphenols were identified and quantified using HPLC-DAD-ESI-MS. The antioxidant activity was evaluated with DPPH and ß-carotene tests, and the anti-inflammatory activity with inhibition of bovine serum albumin denaturation and HRBC membrane stabilization methods. The results showed that Ruta graveolens extract is rich in phenolic compounds with a total phenol and flavonoid contents of 41.63±0.394â mg GAE/gE and 13.97±0.33â mg EQ/gE, respectively. Nine phenolic compounds were determined, including three phenolic acids and six flavonoids. Rutin was the major phenolic compound in Ruta graveolens (464.95â µg/g), followed by syringic acid (179.74â µg/g), and naringenin (109.78â µg/g). R. graveolens phenolic extract also showed good antioxidant activity with values of 0.77â mM TE/g DW and 0.37â mM ß-CE/g DW with DPPH and ß-carotene tests, respectively. For the anti-inflammatory activity, the highest tested concentration (200 µg/mL) gave 50.61 % of inhibition of the denaturation of albumin and 44.12 % of membrane stabilization. With regards to antimicrobial results, Staphylococcus aureus was the most sensitive bacteria with an inhibition zone of 14.37 mm and MIC value of 0.625 mg/mL, followed by Listeria monocytogenes (11.75 mm and MIC=1.25â mg/mL), and Escherichia coli (10.25 mm and MIC=1.25â mg/mL).