Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Más filtros

Bases de datos
País/Región como asunto
Tipo del documento
País de afiliación
Intervalo de año de publicación
1.
Food Chem ; 442: 138492, 2024 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-38245986

RESUMEN

In this work, we propose a novel approach for extracting Cu and Ni from vegetable oils (which can be expanded to other metals). The method is based on the transference of the analytes to an aqueous acid phase due to the disruption of a three-component solution. The extraction was carried out in two steps. In the first step, a three-component solution was prepared comprising the sample, 1-octanol, and HNO3 solution. Next, the homogeneous system was disrupted by adding 1.0 mL of deionized water, and two phases were formed. The aqueous extract deposited in the bottom of the flask was collected with a micropipette, and Cu and Ni were determined by graphite furnace atomic absorption spectrometry (GF AAS). The developed method presented limits of quantification (LOQ) of 0.25 and 0.17 ng g-1 for Cu and Ni, respectively, and was successfully applied in the analysis of eleven oil samples from different origins.


Asunto(s)
Grafito , Aceites de Plantas , Aceites de Plantas/química , Grafito/química , Espectrofotometría Atómica/métodos , Agua/química
2.
Nutr J ; 14: 54, 2015 May 29.
Artículo en Inglés | MEDLINE | ID: mdl-26022214

RESUMEN

OBJECTIVES: To investigate the effect of partially defatted Granulated Brazil nut (GBN) on biomarkers of oxidative stress and antioxidant status of hypertensive and dyslipidemic patients on nutrition and drug approaches. METHODS: Ninety one hypertensive and dyslipidemic subjects of both genders (51.6 % men), mean age 62.1 ± 9.3 years, performed a randomized crossover trial, double-blind, placebo controlled. Subjects received a diet and partially defatted GBN 13 g per day (≈227.5 µg/day of selenium) or placebo for twelve weeks with four-week washout interval. Anthropometric, laboratory and clinic characteristics were investigated at baseline. Plasma selenium (Se), plasma glutathione peroxidase (GPx3) activity, total antioxidant capacity (TAC), 8-epi PGF2α and oxidized LDL were evaluated at the beginning and in the end of each intervention. RESULTS: GBN intake significantly increased plasma Se from 87.0 ± 16.8 to 180.6 ± 67.1 µg/L, increased GPx3 activity in 24,8% (from 112.66 ± 40.09 to 128.32 ± 38.31 nmol/min/mL, p < 0,05), and reduced 3.25% of oxidized-LDL levels (from 66.31 ± 23.59 to 60.68 ± 20.88 U/L, p < 0.05). An inverse association between GPx3 and oxidized LDL levels was observed after supplementation with GBN by simple model (ß -0.232, p = 0.032) and after adjustment for gender, age, diabetes and BMI (ß -0.298, p = 0.008). There wasn't association between GPx3 and 8-epi PGF2α (ß -0.209, p = 0.052) by simple model. CONCLUSION: The partially defatted GBN intake has a potential benefit to increase plasma selenium, increase enzymatic antioxidant activity of GPx3 and to reduction oxidation in LDL in hypertensive and dyslipidemic patients. TRIAL REGISTRATION: ClinicalTrials.gov Identifier NCT01990391; November 20, 2013.


Asunto(s)
Antioxidantes/metabolismo , Bertholletia/química , Dislipidemias/sangre , Hipertensión/sangre , Nueces/química , Anciano , Biomarcadores/sangre , Presión Sanguínea , Índice de Masa Corporal , Peso Corporal , HDL-Colesterol/sangre , LDL-Colesterol/sangre , Estudios Cruzados , Dieta , Método Doble Ciego , Dislipidemias/dietoterapia , Femenino , Glutatión Peroxidasa/sangre , Humanos , Hipertensión/dietoterapia , Modelos Lineales , Masculino , Persona de Mediana Edad , Estado Nutricional , Estrés Oxidativo , Selenio/sangre , Encuestas y Cuestionarios , Triglicéridos/sangre
3.
Int J Environ Res Public Health ; 11(10): 10851-67, 2014 Oct 17.
Artículo en Inglés | MEDLINE | ID: mdl-25329536

RESUMEN

Aging is often accompanied by cognitive impairments and influenced by oxidative status and chemical imbalances. Thus, this study was conducted to examine whether age-related cognitive deficit is associated with oxidative damage, especially with inhibition of the enzyme delta-aminolevulinate dehydratase (ALA-D), as well as to verify the influence of some metals in the enzyme activity and cognitive performance. Blood ALA-D activity, essential (Fe, Zn, Cu, Se) and non-essential metals (Pb, Cd, Hg, As, Cr, Ni, V) were measured in 50 elderly and 20 healthy young subjects. Cognitive function was assessed by tests from Consortium to Establish a Registry for Alzheimer's Disease (CERAD) battery and other. The elderly group presented decreased ALA-D activity compared to the young group. The index of ALA-D reactivation was similar to both study groups, but negatively associated with metals. The mean levels of essential metals were within the reference values, while the most toxic metals were above them in both groups. Cognitive function impairments were observed in elderly group and were associated with decreased ALA-D activity, with lower levels of Se and higher levels of toxic metals (Hg and V). Results suggest that the reduced ALA-D activity in elderly can be an additional factor involved in cognitive decline, since its inhibition throughout life could lead to accumulation of the neurotoxic compound ALA. Toxic metals were found to contribute to cognitive decline and also to influence ALA-D reactivation.


Asunto(s)
Trastornos del Conocimiento/epidemiología , Cognición , Inhibidores Enzimáticos/toxicidad , Metales Pesados/sangre , Estrés Oxidativo , Porfobilinógeno Sintasa/sangre , Adulto , Anciano , Brasil/epidemiología , Trastornos del Conocimiento/sangre , Trastornos del Conocimiento/enzimología , Inhibidores Enzimáticos/sangre , Femenino , Humanos , Masculino , Metales Pesados/toxicidad , Persona de Mediana Edad , Porfobilinógeno Sintasa/antagonistas & inhibidores , Selenio/deficiencia
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA