RESUMEN
Spring rapeseed transgenic lines expressing human interferon alpha 2b were created by Agrobacterium-mediated transformation of aseptic plant leaf explants. The maximum antiviral activity of the leaf extracts reached 4500 IU/g fresh weight. It was determined that the antioxidant activity and the activity of an enzyme of plant antioxidant system--superoxide dismutase (SOD)--in the leaf tissues of transgenic plants increased compared to controls. There were no correlations between the interferon and antioxidant activities, as well as between SOD and interferon activities. Using the obtained transgenic rapeseed plants with high interferon and antioxidant activities as a feed additive for animals might have preventive effect on their body, increasing resistance to infections of various origins.
Asunto(s)
Brassica napus/genética , Interferón-alfa/genética , Plantas Modificadas Genéticamente/genética , Animales , Antioxidantes/metabolismo , Brassica napus/química , Brassica napus/enzimología , Células Cultivadas , Efecto Citopatogénico Viral/efectos de los fármacos , Humanos , Interferón alfa-2 , Extractos Vegetales/farmacología , Hojas de la Planta/enzimología , Hojas de la Planta/genética , Plantas Modificadas Genéticamente/química , Plantas Modificadas Genéticamente/enzimología , Reacción en Cadena de la Polimerasa , Proteínas Recombinantes/genética , Superóxido Dismutasa/metabolismo , Porcinos , Transformación Genética , Virus de la Estomatitis Vesicular Indiana/efectos de los fármacosRESUMEN
The problems and achievements in the rapeseed Brassica napus L. var. oleifera breeding directed on the change of fatty acid composition in seed oil with the use of traditional and genetic engineering approaches are analyzed. It is noticed that the combination of biotechnological workings out and methods of classical breeding is the optimum for the further improvement of rapeseed oil composition.
Asunto(s)
Biotecnología/métodos , Brassica rapa/genética , Ácidos Grasos/análisis , Aceites de Plantas/química , Selección Genética , Brassica rapa/crecimiento & desarrollo , Ácidos Grasos Monoinsaturados , Aceites de Plantas/normas , Aceite de Brassica napusRESUMEN
Transposon mediated insertional mutagenesis is one of the approaches for the unique gene cloning. A wild species of Cruciferae family Orychophragmus violaceus (L.) O.E. Schulz, which is of interest for practical breeding as a donor of improved plant oil, was an object of the investigation. Plasmid construction used in the experiments included selective NPT II gene, reported GUS gene serving as an excision marker, structural BAR gene located within the dSpm element and Spm transposase. The GUS gene of this plasmid had not his own promoter and became functional only after Spm-transposition. Transformed Orychophragmus violaceus (L.) O.E. Schulz. plants were obtained by direct mesophyll protoplast transformation as well as Agrobacterium tumefaciens-mediated root explant transformation. Gene transfer and the transposition event were confirmed by the GUS activity and the PCR analysis. Relative transformation efficiency using protoplasts was 5.8%.
Asunto(s)
Agrobacterium tumefaciens/genética , Brassicaceae/genética , Elementos Transponibles de ADN/genética , Plantas Modificadas Genéticamente/genética , Transformación Genética , Brassicaceae/enzimología , Genes de Plantas/genética , Genes Reporteros/genética , Vectores Genéticos , Raíces de Plantas/enzimología , Raíces de Plantas/genética , Plantas Modificadas Genéticamente/enzimología , Reacción en Cadena de la Polimerasa , Protoplastos/metabolismo , Selección Genética , Transposasas/genéticaRESUMEN
A new generation of synthetic carbon adsorbents was used in production of deliganded human serum albumin preparation. Thermal effects of officinal and deliganded albumin interaction with specific chemical markers were analyzed by flow microcalorimetry. The results demonstrated a 2.5-fold increase of the complexing ability for the deliganded one. The detoxifying potentials of deliganded albumin were studied in comparison with officinal preparation in rats with burn toxemia after IIIB-IV degree thermal injury and in model experiments with blood serum of patients after severe thermal burn. The transfusion of a 5% officinal albumin solution in rats 1 h after burn trauma resulted in a decrease of serum and liver cytosols cytotoxicity 2.2 and 2.4 times, respectively, in comparison with those of burned rats. After deliganded albumin transfusion the cytotoxic activity of blood serum dropped 8.5 times and that of the liver cytosols 18.5 times. The incubation of blood serum of injured patients with equal amounts of a 5% solutions of officinal or deliganded albumin resulted in a fall of the cytotoxicity level and the growth of binding ability. A comparative analysis of detoxifying potentials of albumin preparations has unambiguously demonstrated deliganded albumin advantages.