RESUMEN
The prevalent drugs for treatment of kala azar viz. sodium stibogluconate (SSG) and pentamidine cause severe toxic side effects and acute immunosuppression in the treated individuals. Picroliv, a standardized mixture of iridoid glycosides, prepared from the alcoholic extract of the root and rhizome of Picrorhiza kurroa has shown strong hepatoprotective activity against several models of hepatotoxicity. Therefore, the present study was undertaken with an objective to study the effects of Picroliv (12.5 mg/kg x 7 days oral) alone and in combination with SSG on parasitemia, lipid peroxidation and hepatic marker enzymes of golden hamsters during Leishmania donovani infection. The results indicated a marked hepatoprotective effect of Picroliv in terms of biochemical markers, and a significant antileishmanial activity implying that it can be utilized as an adjunct to chemotherapy or in combination therapy of kala azar along with sodium stibogluconate, thus enhancing the efficacy of antileishmanials.
Asunto(s)
Gluconato de Sodio Antimonio/administración & dosificación , Antiprotozoarios/administración & dosificación , Cinamatos/administración & dosificación , Glicósidos/administración & dosificación , Leishmania donovani , Leishmaniasis Visceral/prevención & control , Mesocricetus/parasitología , Extractos Vegetales/administración & dosificación , Ácido Vanílico/administración & dosificación , Administración Oral , Animales , Bilirrubina/metabolismo , Cricetinae , Quimioterapia Combinada , Glucosa-6-Fosfatasa/metabolismo , Glutamato Sintasa/metabolismo , Leishmaniasis Visceral/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Hígado/efectos de los fármacos , Hígado/enzimología , Succinato Deshidrogenasa/metabolismoRESUMEN
Daily administration of ethyl alcohol (3.76 g/kg, p.o.) for 45 days resulted in significant changes in several biochemical parameters of the liver and serum of albino rats. After exposure to alcohol for 30 days when Picroliv (12 mg/kg, p.o.), an iridoid glycoside fraction of Picrorhiza kurroa, was administered for 15 days along with alcohol, the degree of change in most of the parameters was reduced.
Asunto(s)
Cinamatos/uso terapéutico , Glicósidos/uso terapéutico , Hepatopatías Alcohólicas/prevención & control , Hígado/enzimología , Plantas Medicinales , Ácido Vanílico/uso terapéutico , Alcohol Deshidrogenasa/metabolismo , Aldehído Oxidorreductasas/metabolismo , Animales , Antioxidantes/metabolismo , Etanol/toxicidad , Hígado/efectos de los fármacos , Hígado/patología , Hepatopatías Alcohólicas/metabolismo , Masculino , Extractos Vegetales , RatasRESUMEN
Seven unconjugated steroids were measured in the blood and seminal plasmas of fertile male rabbits by radioimmunoassay. The blood plasma testosterone concentration was 4--5 times that of the seminal plasma. Dehydroepiandrosterone, estrone and 17beta-estradiol were found in measurable amounts in the blood plasma; however, these steroid levels were slightly lower in seminal plasma. Androstenedione and 5alpha-dihydrotestosterone were present in equal quantities in both the seminal and blood plasmas. By contrast, seminal plasma pregnenolone level was about twice that of the blood plasma. The determination of seminal plasma steroids may lend itself as a complementary assessment to blood steroid determinations for the evaluation of the normal function of various reproductive organs.
Asunto(s)
Hormonas Esteroides Gonadales/metabolismo , Pregnenolona/metabolismo , Semen/metabolismo , Androstenodiona/metabolismo , Animales , Deshidroepiandrosterona/metabolismo , Dihidrotestosterona/metabolismo , Estradiol/metabolismo , Estrona/metabolismo , Hormonas Esteroides Gonadales/sangre , Masculino , Pregnenolona/sangre , Conejos , Radioinmunoensayo , Testosterona/metabolismoRESUMEN
PIP: The petroleum ether, alcoholic and aqueous extracts of Apium graveolens Linn., Butea monosperma Lam. Kuntz., and Gossypium herbaceum Linn., the aqueous extract of Aloe Barbadensis Mill.Syn., and the juice of unripe fruits of Ananas comosus were tested on albino rats by a method which detects any antizygotic, blastocystotoxic, antiimplantation, and early abortifacient activity. The extracts were administered for 1-7 days. The dosages for A. graveolens, B. monosperma, and G. herbaceum were 100 mg/kg. 50 ml of A. comosus juice was administered daily. Dosages of 100, 200, and 500 mg/kg of A. barbadensis were given. With the exception of A. comosus, none of the plants showed any antiimplantation activity. The juice of the unripe fruits of A. comosus demonstrated encouraging antiimplantation activity showing 40% of implants only.^ieng