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Isotopes Environ Health Stud ; 51(4): 488-96, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26499512

RESUMEN

Three resistant starches (RSs), namely fibre of potatoes (FP), wrinkle pea starch (WPS), and high amylose maize starch (HAMS) with different dietary fibre contents, were supplemented in adults to evaluate their effects on urinary nitrogen and ammonia excretion as well as on faecal nitrogen excretion by means of lactose-[(15)N2]ureide ((15)N-LU) degradation. Twenty subjects received a regular diet either without or with the supplementation of FP, WPS, and HAMS in a randomized order. After administration of (15)N-LU, urine and faeces were collected over 48 and 72 h, respectively, whereas blood was collected after 6 h. The (15)N-abundances were measured by isotope ratio mass spectrometry. In comparison to the dry run, supplementation with RS significantly lowered renal (15)N-excretion (dry run: 43.2%, FP: 34.6%, WPS: 37.9%, HAMS: 36.4%) as well as the corresponding (15)NH3-excretion (dry run: 0.08%, FP: 0.06%, HAMS: 0.05%), clearly indicating a reduced colonic nitrogen generation at high dietary fibre intake.


Asunto(s)
Amoníaco/metabolismo , Carbohidratos de la Dieta/metabolismo , Pisum sativum/química , Solanum tuberosum/química , Zea mays/química , Adulto , Amoníaco/sangre , Amoníaco/orina , Colon/metabolismo , Carbohidratos de la Dieta/administración & dosificación , Fibras de la Dieta/administración & dosificación , Fibras de la Dieta/metabolismo , Suplementos Dietéticos/análisis , Heces/química , Femenino , Humanos , Lactosa/análisis , Lactosa/sangre , Lactosa/orina , Masculino , Nitrógeno/sangre , Nitrógeno/metabolismo , Nitrógeno/orina , Isótopos de Nitrógeno/análisis , Isótopos de Nitrógeno/sangre , Isótopos de Nitrógeno/orina , Urea/análogos & derivados , Urea/análisis , Urea/sangre , Urea/orina , Adulto Joven
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