A Randomized Trial Using 3Mixtatin Compared to MTA in Primary Molars with Inflammatory Root Resorption: A Novel Endodontic Biomaterial.

OBJECTIVES: Novel methods for preserving primary teeth can help to maintain their developmental, esthetic, and functional capabilities. The aim of this study was to assess the success of the repair of bony defects, caused by pre-treatment perforations, with a mixture of three antibiotics combined with simvastatin (3Mixtatin) compared to MTA in hopeless primary molars. STUDY DESIGN: In this randomized clinical trial, 80 teeth from 65 healthy children aged 3-6 years with interradicular or periapical root resorption and/or perforation in primary molars were treated either with 3Mixtatin or MTA before conventional pulpectomy and restoration. The subjects were followed up clinically and radiographically for 4, 6, 12 and 24 months after pulp treatment to evaluate and compare the healing process. The data were compared using chi-square test at a significance level of 0.05. RESULTS: By the end of 24 months in 3Mixtatin group, 31 (96.8%) teeth revealed no clinical signs or symptoms with arrested resorption progress in radiographs. In MTA group, clinical signs and symptoms including pain, mobility and sinus tract were observed in 18 (48.6%) teeth with cessation of root/interradicular radiolucency in 7 (18.9%) teeth without bone repair. CONCLUSIONS: Radiographic and clinical healing occurred more successfully following 3Mixtatin treatment compared to treatment with MTA, it may lead to a paradigm shift in the pulpal treatment of primary teeth in the future.

Antibacterial efficacy of polymer containing nanoparticles in comparison with sodium hypochlorite in infected root canals.

AIM: The aim of the present study was to compare the antibacterial properties of PLGA polymer containing ZnO, Ag and ZnO/Ag nanoparticles with those of 2.5% NaOCl in root canals contaminated with Enterococcus faecalis. METHODS: A total of 100 maxillary central incisors were selected. After the crowns were removed to achieve 12-mm-length roots, the root canals were prepared with RaCe rotary system. The samples were sterilized; then 200 µL of E. faecalis suspension (ATCC 29212) was placed in each root canal. The samples were divided into 5 groups based on the antibacterial agent used. Group 1 served as the control group. In group 2 the polymer suspension containing ZnO nanoparticles, in group 3 the polymer solution containing ZnO/Ag nanoparticles, in group 4 the polymer solution containing Ag nanoparticles and in group 5, 2.5% NaOCl were used. After 24 hours, a solution was prepared using dentin chips from each root canal and placed on agar plates, followed by colony-forming units (CFU) count determination. Means ± standard deviations were calculated and Kruskal-Wallis test was used to evaluate the antibacterial properties of different irrigation agents. Mann-Whitney U test was used for two-by-two comparisons of the groups, with the level of significance being set at 0.05. RESULTS: The maximum and minimum CFU counts were observed in the control and NaOCl groups, respectively. In the polymer groups, the maximum and minimum CFU counts were observed in the Ag and ZnO/Ag groups, respectively. There were significant differences in CFU counts between the study groups after application of irrigation solutions (P<0.05). Two-by-two comparisons of the groups using Mann-Whitney U test showed significant differences between all the study groups (P<0.05), except for groups ZnO and Ag (P=0.7). CONCLUSION: Sodium hypochlorite solution was more effective than copolymer containing nanoparticles and of all the tested nanoparticles; ZnO/Ag nanoparticles exhibited the highest antibacterial activity.