Mechanism of Brassica oleracea performance in bovine infectious mastitis by bioinformatic analysis.

Bovine mastitis affects dairy cattle worldwide and, despite the existing therapeutic measures, is not totally under control, leading to the need to develop alternative strategies. Brassica oleracea is a phytochemical commonly used in the control and prevention of human and animal diseases. The use of this plant in the treatment of infectious bovine mastitis has been little referenced in the literature and its molecular mechanism of action in this disease has not been clarified yet. This study aimed to reveal, through bioinformatic analysis, the molecular mechanism of action of Brassica oleracea in bovine mastitis. We investigated genes expressed in the signaling pathways of bovine mastitis and Brassica oleracea performance and elaborated the Venn diagram. A gene network was developed using the STRING 10 database. Leader genes were identified by calculating the weighted number of links (WNL). The NetworkAnalyzer plugin for Cytoscape software was used to characterize network topology. For the visualization of highly interconnected regions in the network, the MCODE was used. The BINGO and GFD-Net plugins were used to perform the ontological analysis. The TP53 and MTOR leader genes were identified in the sub-networks of the bovine mastitis signaling pathway and Brassica oleracea performance, respectively. Topological analysis confirmed the leader condition of the genes. Although the overlap of genes in the Venn diagram was not observed, the leader genes were found to be interconnected (confidence = 0.9). In the network that interconnected the leader genes two molecular complexes were detected and the ontological analysis revealed biological processes, cellular components and important molecular functions. It was concluded that Brassica oleracea may be a promising candidate to be included in a mammalian herbal cocktail against infectious bovine mastitis by interfering in the mechanisms of action of genes such as MTOR and TP53.

Toxicidade aguda dos extratos hidroalcoólicos das folhas de alecrim-pimenta, aroeira e barbatimão e do farelo da casca de pequi administrados por via intraperitoneal / Acute toxicity of leaf hydroalcoholic extracts of Lippia sidoides, Myracroduon urundeuva, Stryphnodendron adstringens and of Caryocar brasilliense administered by intraperitoneal route

O estudo objetivou realizar ensaio toxicológico pré-clínico inicial para investigar a toxicidade das folhas de alecrim-pimenta (Lippia sidoides Cham.), aroeira (Myracrodruon urundeuva Fr. All.) e barbatimão [Stryphnodendron adstringens (Mart.) Coville] e do farelo da casca de pequi (Caryocar brasiliense Camb.), por meio da determinação da dose letal 50 por cento (DL50). Na investigação da DL50, foram utilizados grupos de camundongos Swiss de mesmo sexo (n=150, sendo 30 animais por tratamento) inoculados, por via intraperitoneal, com diluições seriadas do extrato hidroalcoólico das folhas de alecrim, aroeira e barbatimão e do farelo da casca de pequi. Após a inoculação, os animais foram observados por um período de 14 dias, para determinar a quantidade de mortos, doentes e sobreviventes. O estudo toxicológico pré-clínico agudo demonstrou, em camundongos por via intraperitoneal, toxicidade igual a 0,31mg mL-1 DL50 mL-1 para todas as plantas, exceto para o barbatimão, que apresentou toxicidade igual a 0,25mg mL-1. Pesquisas devem ser realizadas visando a obter dados de toxicidade das plantas em outras vias para assegurar o uso em saúde humana e animal.

This study aimed to conductpre-clinical toxicology testing to investigate the toxicity of Lippia sidoides Cham., Myracrodruon urundeuva Fr. All., Stryphnodendron adstringens (Mart.) Coville and Caryocar brasiliense Camb., by determining the 50 percent (LDL50) lethal dose. In the investigation of the LD50, groups of Swiss mice of the same sex were used (n=150; 30 animals per plant and all inoculated intraperitoneally with serial dilutions of the aqueous fraction obtained from the leaves of plants. After inoculation, the animals were observed along a period of 14 days in order to observe the dead, sick and survivors rate. In mice inoculated intraperitoneally, the acute pre-clinical toxicology testing demonstrated toxicity equal to 0,31mg mL-1 LD50 mL-1 for all plants. The exception was for Stryphnodendron adstringens, which presented toxicity equal to a 0,25mg mL-1DL50 mL-1 and values expressed in terms of dilution. Research should be conducted to obtain data on toxicity of the plants in other ways to ensure the use in human and animal health. Therefore, the studied plants should be used with caution. They present a relatively mild toxic potential, but it may be harmful to users if they are not properly utilized.