RESUMEN
OBJECTIVES: Although the genotoxicity related to waste anaesthetic gases is controversial, a consistent number of observations have provided evidence for an increased level of DNA strand breaks. The goal of the research was to investigate this hypothesis and estimate the genoprotective role of antioxidant supplementation in technical anaesthesiology staff working in operating theatres. METHODS: Heparinized venous blood samples were collected from 17 exposed technical anaesthesiology staff (mean age 34.3 +/- 3.5 years) and non-exposed control group (mean age 32.2 +/- 3.4 years) and examined in the alkaline comet assay for DNA strand breakage. Vitamin E (300 mg/day) plus vitamin C (500 mg/day) were supplemented to the technical anaesthesiology staff for 12 weeks and blood samples were retaken and evaluated by comet assay. RESULTS: The DNA breakage observed in the lymphocytes of the technical anaesthesiology staff was 21.5 +/- 5.0, as calculated by total comet score (TCS). This score was significantly higher (P<0.001) than in the controls (8.6 +/- 4.7) before antioxidant treatment. Supplementation of vitamins E plus C significantly (P<0.01) reduced the mean TCS as 14.2 +/- 6.1. CONCLUSION: The results of our study indicate that occupational exposure to anaesthetic gases induces oxidative DNA damage. Supplementation of the diet for 12 weeks with vitamin C and vitamin E resulted in a significant decrease in the DNA damage.
Asunto(s)
Anestesiología , Anestésicos por Inhalación/sangre , Antioxidantes/uso terapéutico , Daño del ADN , Suplementos Dietéticos , Residuos Peligrosos/análisis , Exposición Profesional/análisis , Quirófanos , Adulto , Anestésicos por Inhalación/clasificación , Anestésicos por Inhalación/toxicidad , Ensayo Cometa , Factores de Confusión Epidemiológicos , Desflurano , Electroforesis , Femenino , Residuos Peligrosos/efectos adversos , Humanos , Isoflurano/análogos & derivados , Isoflurano/sangre , Isoflurano/toxicidad , Masculino , Éteres Metílicos/sangre , Éteres Metílicos/toxicidad , Óxido Nitroso/sangre , Óxido Nitroso/toxicidad , Exposición Profesional/efectos adversos , Auxiliares de Cirugía , Estrés Oxidativo , Sevoflurano , Fumar , alfa-Tocoferol/uso terapéuticoRESUMEN
Diabetes patients often show increased production of reactive oxidative species (ROS) together with vascular complications. The presence of these ROS may lead to increased DNA damage in peripheral blood lymphocytes that may be revealed by the comet assay. To test whether DNA is damaged in diabetes, peripheral blood samples were taken from 30 control individuals and 63 diabetic patients (15 insulin dependent (IDDM) and 48 non-insulin dependent (NIDDM)) and the alkaline comet assay was used to evaluate background levels of DNA damage. Significant differences were detected between control and diabetic patients in terms of frequencies of damaged cells. The extend of DNA migration was greater in NIDDM patients by comparison with IDDM patients which might indicate that IDDM patients are handling more oxidative damage on a regular basis. Smoker individuals had higher frequencies of cells with migration by comparison with the non-smokers in both groups. Also, clear differences between patients on placebo and on Vitamin E supplementation for 12 weeks were observed on the basis of the extend of DNA migration during single cell gel electrophoresis.
Asunto(s)
Antioxidantes/farmacología , Daño del ADN , Diabetes Mellitus/genética , Suplementos Dietéticos , Linfocitos/efectos de los fármacos , Vitamina E/farmacología , Adulto , Anciano , Antioxidantes/administración & dosificación , Ensayo Cometa , Diabetes Mellitus/sangre , Diabetes Mellitus Tipo 1/sangre , Diabetes Mellitus Tipo 1/genética , Diabetes Mellitus Tipo 2/sangre , Diabetes Mellitus Tipo 2/genética , Electroforesis en Gel de Agar , Femenino , Humanos , Linfocitos/ultraestructura , Masculino , Persona de Mediana Edad , Estrés Oxidativo , Especies Reactivas de Oxígeno , Fumar/sangre , Fumar/genética , Vitamina E/administración & dosificaciónRESUMEN
Sister-chromatid exchange (SCE) analysis was carried out in different age groups prior to and after therapy with 8-Methoxypsoralen (8-MOP) followed by exposure of the patient to long-wave UV-A (PUVA) and compared to control. The SCE frequencies were increased significantly in PUVA-treated patients as compared to their pre-treatment SCE levels and to controls. A significant increase in SCEs was found in smoking PUVA-treated patients as compared to non-smoking PUVA-treated patients. This study indicates a detectable chromosome-damaging effect of PUVA therapy on its human users.
Asunto(s)
Mutagénesis , Terapia PUVA/efectos adversos , Psoriasis/tratamiento farmacológico , Intercambio de Cromátides Hermanas , Adolescente , Adulto , Anciano , Estudios de Casos y Controles , Niño , Femenino , Humanos , Masculino , Persona de Mediana Edad , Fumar/efectos adversosRESUMEN
Aqueous extracts of a different variety of fresh broad bean seeds obtained from a favism endemic area in Turkey, were incubated with blood from sensitive and non-sensitive (control) subjects. Red blood cells were characterized by a whole blood glutathione (GSH) and a deficiency of Glucose-6-phosphate dehydrogenase (G-6-PD) activity. As the decrease in GSH percent is taken as an index of haemolytic activity, the test results were as following: Sakiz , Milas -Region, French broad bean extracts reduced the blood GSH levels 48%, 70%, 46% and 53%, respectively, in favism sensitive subjects. Active principles which are responsible for the haemolysis ( Vicine and Convicine ) were isolated from broad beans and their effects on GSH levels of blood were 99% and 81%, respectively, in favism sensitive subjects and 33.3% and 19% in normal subjects.