RESUMEN
The authors have previously shown that inhibition of the acoustic startle response by a prepulse increases when it is repetitively elicited over days. The present experiments show in C3H and C57 mice that this change is caused by an increase in prepulse inhibition (PPI) and not by a decrease in prepulse facilitation. This PPI increase is only evoked if prepulses and startle stimuli are repeatedly given in a temporally paired ("contingent") order, proposing an associative learning process. (Only in C57 mice, PPI was additionally increased by adaptation in the same, but not in a different, context). As an underlying mechanism for this PPI increase by experience, the authors hypothesize Hebbian plasticity of an inhibitory synapse.
Asunto(s)
Estimulación Acústica/métodos , Habituación Psicofisiológica , Inhibición Psicológica , Reflejo de Sobresalto/fisiología , Animales , Animales Recién Nacidos , Femenino , Ratones , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Factores de TiempoRESUMEN
The influence of prepulses on the acoustic startle response (ASR) was measured in three inbred mouse strains, C57BL/6J, 129/SvHsd, and AKR/OlaHsd, and one hybrid strain produced by crossing wild mice and NMRI mice. Prepulse inhibition (PPI), i.e. reduction of ASR by prepulses, was maximal when the interval between prepulses and startle stimuli was in the range of 37.5-100 ms. Prepulse facilitation (PPF), i.e. increase of ASR by prepulses, was maximal when the prepulse preceded the startle stimulus by 12.5 ms. PPI increased with increasing prepulse SPL, PPF first increased then decreased when prepulse SPL was increased. Percent PPI was independent from startle stimulus SPL. All strains showed a long-term increase of PPI when tested for several days; one strain (129) also showed an increase of PPF over days. The present results clearly show that PPI and PPF are independent processes, which add to yield the final response change. PPF and the observed long-term changes of PPI and PPF are stronger expressed in mice than have been observed in rats under similar conditions. Since there were significant differences between the strains of mice with respect to PPI and PPF, genetically different strains of mice are a promising tool to study these two processes.
Asunto(s)
Inhibición Psicológica , Reflejo de Sobresalto/fisiología , Estimulación Acústica/métodos , Análisis de Varianza , Animales , Conducta Animal/efectos de la radiación , Quimera/genética , Quimera/fisiología , Relación Dosis-Respuesta en la Radiación , Femenino , Genética Conductual , Masculino , Ratones , Ratones Endogámicos , Ratas , Reflejo de Sobresalto/genética , Reflejo de Sobresalto/efectos de la radiación , Especificidad de la Especie , Factores de TiempoRESUMEN
The present study examined the role of the perirhinal cortex (PRh) in aversive information processing and emotional learning. Specifically, we studied the effects of temporary inactivation of the PRh on acquisition and expression of conditioned fear as measured by fear-potentiated startle in rats, as well as on shock sensitization of startle. Temporary inactivation of the PRh was induced by local injections of the GABAA agonist muscimol (0.0, 1.1, 2.2, 4.4 nmol/0.5 micro L). Muscimol injections into the PRh blocked both the expression and acquisition of fear-potentiated startle, as well as shock sensitization of startle. Shock sensitivity was not affected by muscimol injections, indicating that the observed blockade of acquisition and shock sensitization was not caused by a disruption in the perception of shock. Taken together, the present data show that the PRh is critical for the processing of aversive information and is necessary for the expression of emotional learning.
Asunto(s)
Corteza Cerebral/efectos de los fármacos , Miedo/efectos de los fármacos , Agonistas del GABA/farmacología , Muscimol/farmacología , Reflejo de Sobresalto/efectos de los fármacos , Estimulación Acústica , Animales , Conducta Animal , Corteza Cerebral/anatomía & histología , Condicionamiento Clásico , Relación Dosis-Respuesta a Droga , Electrochoque/métodos , Masculino , Dimensión del Dolor/efectos de los fármacos , Estimulación Luminosa , Ratas , Ratas Sprague-Dawley , Tiempo de Reacción/efectos de los fármacosRESUMEN
The startle response is an important mammalian model for studying the cellular mechanisms of emotions and of learning. It consists of contractions of facial and skeletal muscles in response to sudden acoustic, tactile or vestibular stimuli. Whereas the acoustic startle pathway is well described, only a few recent studies have investigated the tactile startle pathway. It was proposed that there is a direct projection from the principal sensory nucleus to the central sensorimotor interface of the startle response, which is formed by the giant neurons in the caudal pontine reticular formation. We explored this projection in greater detail in vitro. Anterograde tracing in rat brain slices confirmed projections with large axon terminals from the ventral part of the principal sensory nucleus to the lateral caudal pontine reticular formation. Electrophysiological studies revealed a monosynaptic glutamatergic connection between principal sensory nucleus neurons and caudal pontine reticular formation giant neurons. The synapses displayed paired-pulse facilitation at high-frequency stimulation, and homosynaptic depression at 1 Hz stimulation. The latter form of plasticity is thought to underlie habituation of the startle response. Furthermore, postsynaptic currents in caudal pontine reticular formation giant neurons evoked by principal sensory nucleus neuron stimulation summed in a linear way with signals evoked by stimulation of auditory afferents. Synaptic plasticity and summation of synaptic currents correspond well with in vivo data previously published by other groups. We thus presume that these synapses mediate trigeminal input to the startle pathway.
Asunto(s)
Tronco Encefálico/fisiología , Reflejo de Sobresalto/fisiología , Nervio Trigémino/fisiología , Valina/análogos & derivados , 6-Ciano 7-nitroquinoxalina 2,3-diona/farmacología , Estimulación Acústica , Animales , Animales Recién Nacidos , Carbocianinas/metabolismo , Estimulación Eléctrica , Antagonistas de Aminoácidos Excitadores/farmacología , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Colorantes Fluorescentes/metabolismo , Antagonistas del GABA/farmacología , Glicinérgicos/farmacología , Habituación Psicofisiológica/fisiología , Técnicas In Vitro , Neuronas Aferentes/fisiología , Técnicas de Placa-Clamp , Picrotoxina/farmacología , Ratas , Ratas Sprague-Dawley , Periodo Refractario Electrofisiológico/fisiología , Estricnina/farmacología , Transmisión Sináptica/fisiología , Valina/farmacologíaRESUMEN
The aim of the present study was to analyse the cellular mechanism underlying short-term habituation of the acoustic startle response (ASR). We explored distinct synapses of the neuronal startle pathway in rat brain slices by patch-clamp recordings of giant neurons in the caudal pontine reticular formation. Presynaptic stimulation of auditory afferents by repeated bursts at 0.1 and 1 Hz led to an exponential decay of EPSC magnitudes. This homosynaptic depression (HSD) was reversible and repeatedly inducible after recovery. Many parameters of HSD in vitro match those of ASR habituation in vivo. The mechanisms underlying HSD are distinct from classical short-term plasticity: paired-pulse as well as paired-burst stimulation revealed a facilitation of the second EPSC, occurring in a much smaller time window up to interstimulus intervals of 200 ms. Pharmacological experiments demonstrated that HSD could be completely blocked by the group II and III metabotropic glutamate receptor antagonist MPPG. Similar results were obtained by CPPG, another group II and III antagonist. In contrast, HSD was not affected by the group I and II antagonist MCPG. We conclude that we found a form of synaptic depression in synapses within the primary startle pathway which correlates in many respects with short-term habituation of the ASR and which is presumably mediated by group III metabotropic glutamate receptors.
Asunto(s)
Alanina/análogos & derivados , Glicina/análogos & derivados , Habituación Psicofisiológica/fisiología , Plasticidad Neuronal , Neuronas/fisiología , Puente/fisiología , Reflejo de Sobresalto/fisiología , Sinapsis/fisiología , Estimulación Acústica , Alanina/farmacología , Animales , Potenciales Postsinápticos Excitadores/fisiología , Glicina/farmacología , Depresión Sináptica a Largo Plazo/efectos de los fármacos , Depresión Sináptica a Largo Plazo/fisiología , Neuronas/efectos de los fármacos , Técnicas de Cultivo de Órganos , Técnicas de Placa-Clamp , Puente/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Formación Reticular/efectos de los fármacos , Formación Reticular/fisiología , Sinapsis/efectos de los fármacosRESUMEN
Numerous studies of aversive learning with different animal models have shown that the noradrenergic system has an important role in the acquisition, consolidation and expression of aversive learning. We used intracerebral clonidine injections to investigate the role of the noradrenergic amygdaloid system in the fear-potentiated startle paradigm. Clonidine is a noradrenergic alpha2-receptor agonist which can decrease noradrenergic transmission by stimulating presynaptic alpha2-receptors. Rats received injections of 0, 2.5, 5 and 10 nmol clonidine into the lateral amygdala (i) before fear-conditioning, (ii) immediately after fear-conditioning, (iii) before testing and (iv) before both fear-conditioning and the testing of conditioned fear. Clonidine injections blocked the acquisition and expression of conditioned fear. The effect on acquisition was not caused by state-dependency or possible side-effects of clonidine on consolidation. Given that clonidine decreases amygdaloid noradrenaline release, these results show a crucial role of noradrenergic transmission within the amygdala in classical fear-conditioning. Surprisingly, both the acquisition and the expression of conditioned fear were blocked after amygdaloid injections of clonidine, suggesting that amygdaloid noradrenaline is necessary to induce both unconditioned and conditioned fear.