RESUMEN
Analytical electron microscopy is an ideal tool for holistic data acquisition on biological systems. The use of analytical electron microscopy for both, the investigation of micropharmacokinetic problems and metabolic studies, is becoming more and more important. Depending on the mode of investigation, it is possible to localize drugs and xenobiotics precisely in situ under optical control or to quantify their uptake and distribution in the corresponding target cells without disintegrating the cell or tissue material. In this paper, we present instructive examples for the application of analytical electron energy loss spectroscopy in transmission electron microscopy in order to investigate the cellular uptake and distribution of cisplatin and cyclophosphamide and the metabolic changes induced by an alteration in the extracellular calcium concentration in a holistic manner.
Asunto(s)
Antineoplásicos/farmacocinética , Cisplatino/farmacocinética , Criopreservación/métodos , Ciclofosfamida/farmacocinética , Microanálisis por Sonda Electrónica/métodos , Neoplasias/metabolismo , Animales , Cisplatino/química , Ciclofosfamida/química , Resistencia a Antineoplásicos , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patología , Neoplasias Pulmonares/ultraestructura , Ratones , Ratones Desnudos , Microscopía Electrónica/métodos , Neoplasias/tratamiento farmacológico , Neoplasias/ultraestructura , Fósforo/análisis , Adhesión en Plástico , Distribución Tisular , Células Tumorales CultivadasRESUMEN
Electron energy loss spectroscopy (EELS) has been used to measure the ratios of C, N, O, P and Ca in ultrathin cryosections from normal and neoplastic gastric tissues. First results show a correlation between the EELS, and morphometric data in cells from these tissues. We have found that ultrathin, freeze-dried cryosections, with an average thickness of up to 75 nm, are stable enough for EELS-analysis in a 200 KV electron microscope with an adapted Gatan-EELS-Spectrometer.