Determination of arctiin and arctigenin in Fructus Arctii by reverse-phase HPLC.

The fruits of Arctium lappa L. is an often-used herbal drug in traditional Chinese medicine for the treatment of common cold caused by wind and heat. This drug contained many constituents, principally arctiin, with arctigenin in smaller amount. In this work, arctiin has been isolated from the fruits of Arctium lappa, and then enzymolyzed into arctigenin. The obtained arctiin and arctigenin were characterized and then used as standards for their determination in the crude drug by HPLC. The method is simple, rapid and accurate.

[Isolation and structure determination of a new saponin of Anemarrhena asphodeloides].

A new saponin, named smilageninoside, mp 265-7 degrees C, was isolated from rhizomes of Anemarrhena asphdeloides by conventional method. The structure of smilageninoside was identified on the basis of IR, 1HNMR, 13CNMR and FAB-MS. Its structure was smilagenin 3-O-[beta-D-glucopyranosyl (1----2)]-beta-D-mannopyranoside.

[Studies on the saponins from the root bark of Aralia chinensis L].

Six saponins were isolated from the root bark of Aralia chinensis L.. The three saponins I, II and III were identified as araloside A (I), narcissiflorine (II) and oleanolic acid 3-O-beta-D-glucopyranosyl-(1-2)-beta-D-xylopyranosyl-(1-2)-alpha-L- arabinopyranoside (III). Saponin III was found to be a new compound and was named as araloside D.

[Studies on the saponin constituents of Kalopanax septemlobus (Thunb.) koidz].

Two crystalline components I and II were isolated from the total saponin of Kalopanax septemlobus (Thunb.) koidz. Crystalline II was identified as kalopanax saponin A and I was named as Kalopanax saponin C and eluciated as 3-O-[alpha-L-rhamnopyranosyl-(1----2)-alpha-L-arabinopyranosyl]- hedragenin-28-[alpha-L-rhamnopyranosyl-(1----2)- beta-D-glucopyranosyl (1----6)-beta-D-glucopyransyl]ester on the basis of spectral data (13CNMR, 1HNMR, IR and FAB-MS).

[Determination of osthol and imperatorin in Cnidium monnieri (L.) cuss by fluorometry TLC scanning].

The main active constituents--osthol and imperatorin were isolated from Cnidium monnieri (L.) Cuss and then used as standards for their determination in the crude drug by fluorometry TLCS. The fructus Cnidii was macerated with ethanol overnight and the ethanolic extract was spotted on a silica gel G-CMC Na plate. The plate was developed with n-hexane-ethyl acetate (7:3). The spot was excited by light of 365 nm, and determined in a Shimadzu CS-930 TLC scanner using a No. 2 filter. The peak areas of the two components were obtained, external standard method was used in the determination. The root, stem, leaf and flower of this plant were shown to contain no osthol and imperatorin, and the seed was found to contain 2.0-3.2% of osthol and 1.8-2.5% of imperatorin.

[TLC-densitometric determination of sarsasapogenin in Anemarrhena asphodeloides Bge].

The sarsasapogenin content in Anemarrhena asphodeloides and its preparations was assayed by TLC-densitometric method. The developing solvent used was benzene-acetone (9:1). The chromatographic plate was sprayed with a mixture of 8% vanillin in alcoholic solution-70% H2SO4 solution (0.5:5). This plate was scanned using 445 nm as wavelength lambda s and 600 nm as wavelength lambda s.

[Studies on the chemical constituents of Aconitum szechenyianum Gay].

Five constituents were isolated from Aconitum szechenyianum Gay. The structures of these compounds were identified as beta-sitosterol (I), aconitine (II), 3-acetylaconitine (III), songorine (IV) and a new compound whose structure was elucidated to be szechenyine (V) on the basis of UV, IR, 1HNMR, 13CNMR and Mass spectra.