RESUMEN
To help prevent foodborne enteritis in aquaculture, several feed additives, such as herbal medicine, have been added to fish diets. Predictions of effective herb medicines for treating fish foodborne enteritis from key regulated DEGs (differentially expressed genes) in transcriptomic data can aid in the development of feed additives using the Traditional Chinese Medicine Integrated Database. Seabuckthorn has been assessed as a promising candidate for treating grass carp soybean-induced enteritis (SBMIE). In the present study, the SBMIE zebrafish model was used to assess seabuckthorn's therapeutic or preventative effects. The results showed that intestinal and hepatic inflammation was reduced when seabuckthorn was added, either pathologically (improved intestinal villi morphology, less oil-drops) or growth-related (body fat deposition). Moreover, seabuckthorn may block the intestinal p53 signaling pathway, while activating the PPAR signaling pathway and fatty acid metabolism in the liver. 16S rRNA gene sequencing results also indicated a significant increase in OTU numbers and skewed overlapping with the fish meal group following the addition of seabuckthorn. Additionally, there were signs of altered gut microbiota taxa composition, particularly for reduced TM7, Sphingomonas, and Shigella, following the addition of seabuckthorn. Hindgut imaging of fluorescent immune cells in SBMIE larvae revealed the immune regulatory mechanisms at the cellular level. Seabuckthorn may significantly inhibit the inflammatory gathering of neutrophils, macrophages, and mature T cells, as well as cellular protrusions' formation. On the other hand, in larvae, seabuckthorn inhibited the inflammatory aggregation of lck+ T cells but not immature lymphocytes, indicating that it affected intestinal adaptive immunity. Although seabuckthorn did not affect the distribution of intestinal CD4+ cells, the number of hepatic CD4+ cells were reduced in fish from the seabuckthorn supplementation group. Thus, the current data indicate that seabuckthorn may alleviate foodborne gut-liver symptoms by enhancing intestinal mucosal immunity and microbiota while simultaneously inhibiting hepatic adipose disposition, making it a potential additive for preventing fish foodborne gut-liver symptoms.
RESUMEN
This research aimed to study whether Enteromorpha polysaccharide-zinc (EP-Zn) can act as an alternative to antibiotics in weaned piglet feeds. Two hundred and twenty-four weaned piglets from 14 pens were randomly assigned into 1 of 2 groups according to their body weight and litter size (7 pens/group). The piglets in the antibiotics group were fed with olaquindox at 400 mg/kg and enduracidin at 800 mg/kg basal diet, and piglets in the EP-Zn group were fed with EP-Zn at 800 mg/kg basal diet. One piglet per pen was selected to collect samples after 14 d of feeding. Results showed that EP-Zn supplementation significantly increased the plasma anti-oxidants level compared with the antibiotics group. However, a nonsignificant difference was observed in growth performance between treatment groups. Additionally, the intestinal tight junction (TJ) protein expression and the histopathologic evaluation data showed that EP-Zn contributed to improving intestinal development. Further, piglets in the EP-Zn group had a lower level of intestinal inflammation-related cytokines including IL-6 (P < 0.001), IL-8 (P < 0.05), IL-12 (P < 0.05) and tumor necrosis factor-α (TNF-α) (P < 0.001), and showed an inhibition of the phosphorylation nuclear transcription factor-kappa B (p-NF-κB) (P < 0.05) and total NF-κB (P < 0.001) level in the jejunal mucosa. Taken together, it is supposed that EP-Zn, to some extent, would be a potent alternative to prophylactic antibiotics in improving the health status of weaned piglets.