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Métodos Terapéuticos y Terapias MTCI
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Zhonghua Yu Fang Yi Xue Za Zhi ; (12): 290-294, 2007.
Artículo en Chino | WPRIM | ID: wpr-270502

RESUMEN

<p><b>OBJECTIVE</b>To investigate the anti-fibrotic effects of Qidan granule in rats.</p><p><b>METHODS</b>The rats were randomly divided into six experimental groups: normal group, model group, Qidan group, Tetrandrine group. All rats except normal group were treated with silicon dioxide (50 mg/rat) by intratracheal instillation to induce silicosis. Qidan group and Tetrandrine group were treated with Qidan granule (3125 mg/kg) or treated with Tetrandrine (22 mg/kg) respectively. All the rats were sacrificed after 5 months. Calculate Lung/body coefficient by weighting the lung wet weight and the body weight of rats. Content of Hydroxyproline was measured by alkaline hydrolysis. The gene expression of transforming growth factor-beta1 was examined by using enzyme-linked immunosorbent assay (ELISA). Paraffin embedded lung sections with HE staining, VG staining and Gomori staining were observed under light microscope.</p><p><b>RESULTS</b>In Qidan group and Tetrandrine group, Lung/body coefficient and content of Hydroxyproline and expression of transforming growth factor-beta1 were lower as compared with model group (P < 0.05). Model group mainly showed III approximately IV grade silicotic nodule, which contained thick collagen and sparse reticulum fibe; Qidan group and Tetrandrine group appeared with II grade silicotic nodule, which contained tiny collagen and intensive reticulum fibe. Tetrandrine group showed injury of kidney, and others were normal.</p><p><b>CONCLUSION</b>Qidan granule extract should prevent and from inhibit the remarkably silicotic fibrosis in rats.</p>


Asunto(s)
Animales , Ratas , Modelos Animales de Enfermedad , Medicamentos Herbarios Chinos , Farmacología , Usos Terapéuticos , Fitoterapia , Fibrosis Pulmonar , Patología , Ratas Wistar , Silicosis , Quimioterapia , Metabolismo , Patología , Factor de Crecimiento Transformador beta
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