RESUMEN
A 15-run Box-Behnken design (BBD) was used to optimize the extraction conditions of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg. Three factors such as extraction temperature (°C), extraction time (h), and ratio of water to raw material were investigated. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using the appropriate statistical methods. The adjusted coefficient of determination (R(Adj)(2)) for the model was 0.9754, and the probability value (P=0.001) demonstrated a high significance for the regression model. The optimum extraction conditions were found to be: optimized extraction temperature 83.3°C, extraction time 1.55 h and ratio of water to raw material 29.48. Under these conditions, the mean extraction yield of polysaccharides was 5.182 ± 0.093 %, which was in good agreement with the predicted model value.
Asunto(s)
Química Farmacéutica/métodos , Medicamentos Herbarios Chinos/química , Modelos Estadísticos , Raíces de Plantas/química , Polisacáridos/análisis , Vitaceae/química , Algoritmos , Medicamentos Herbarios Chinos/análisis , Análisis de Regresión , Temperatura , AguaRESUMEN
OBJECTIVE: To evaluate the quality of Flos Chrysanthemi Indici which produced in twenty-two different producing places. METHOD: Chlorogenic acid and caffeic acid were analyzed on a Shim-pack C8 colunm (4.6 mm x 250 mm, 5 microm) eluted with the mobile phase consisted of acetonitrile-0.5% phosphoric acid( 19:81). The detection wavelength was set at 326 nm. Linarin were eluted with the mobile phase consisted of methanol-water-acetic acid(26: 23: 1). The detection wavelength was set at 334 nm. The column temperature was 25 degrees C. The flow rate was 1.0 mL x min . RESULT: The linear response ranged within 2.5-50 microg for chlorogenic acid (r = 0.998), 2.5-25 microg for caffeic acid (r = 0.998) and 4.97-41.47 microg for linarin (r = 0.999), respectively. Recoveries were 100.8% with RSD 2.1% for chlorogenic acid, 96.2% with RSD 2.3% for caffeic acid and 103.7% with RSD 1.8% for linarin. CONCLUSION: There was a significant difference in the content of chlorogenic acid, caffeic acid, linarin among the samples. The content of chlorogenic in the sample from Fengdou Chongqing city was the highest in those from other places. The content of caffeic acid in the all samples is very low. The content of linarin in the samples from Jiangsu province and Anhui province almost reached the national standard in pharmacopoeia.
Asunto(s)
Ácidos Cafeicos/análisis , Ácido Clorogénico/análisis , Chrysanthemum/química , Glicósidos/análisis , Extractos Vegetales/análisis , China , Cromatografía Líquida de Alta Presión , Flores/química , Control de CalidadRESUMEN
OBJECTIVE: To develop a RP-HPLC method for the determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi indici. METHOD: An Eclipse XDB-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phases of methanol-0.2% phosphatic acid in a gradient manner. The flow rate was set at 1.0 mL x min(-1). The detection wavelength was 350 nm. RESULT: The linear response ranged from 1.02-20.48 mg x L(-1) for quercetin (r = 0.9994, n = 5), 1.03-20.54 mg x L(-1) for luteolin (r = 0.9992, n = 5), 1.12-22.40 mg x L(-1) for apigenin (r = 0.9995, n = 5), 1.01-20.22 mg x L(-1) for acacetin (r = 0.9998, n = 5), respectively. Recoveries were 101.3% with RSD 1.3% for quercetin, 100.62% with RSD 1.4% for luteolin, 98.42% with RSD 1.7% for apigenin and 99.02% with RSD 0.8% for acacetin. A significant difference (alpha = 0.01) among the contents of four flavonoids and total flavonoids was found. CONCLUSION: The method is quick, simple and repeatable for simultaneous determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Cromatografía de Fase Inversa/métodos , Chrysanthemum/química , Flavonoides/análisis , Extractos Vegetales/análisis , Apigenina/análisis , Flavonas/análisis , Flores/química , Luteolina/análisis , Quercetina/análisisRESUMEN
OBJECTIVE: To study pre-treatment in determining total polysaccharide in flos Chrysanthemi Indici. METHOD: The factors including the extraction temperature, extraction time, ratio of material to liquid were studied. The best extraction condition was found through the response surface design. RESULT: The best extraction condition as follows: 81.0 degrees C of the extraction temperature, 1.6 h of extraction time, and the ratio of material to water as 1: 29. On these conditions the extraction rate of flos Chrysanthemi Indici was the best. CONCLUSION: A model equation that can be used to predict the experiment is established through the response surface method.