RESUMEN
One gram-negative strain designated Bb-Pol-6 T was isolated from birch (Betula pendula) pollen at Giessen area, Germany. The analysis of 16S rRNA gene-based phylogenies indicated the next-relative genera were Robbsia, Chitinasiproducens, Pararobbsia and Paraburkholderia (96-95.6%). Further comparative genome analysis and phylogenetic tree-based methods revealed its phylogenetic position under the genus Robbsia. The genome of strain Bb-Pol-6 T was 5.04 Mbp with 4401 predicted coding sequences and a G + C content of 65.31 mol%. Average amino acid identity, average nucleotide identity, digital DNA-DNA hybridization and percentage of conserved proteins values to Robbsia andropogonis DSM 9511 T were 68.0, 72.5, 22.7 and 65.85%, respectively. Strain Bb-Pol-6 T was rod-shaped, non-motile, facultative anaerobic and grew optimally at 28 °C and pH 6-7. Ubiquinone 8 was the major respiratory quinone and the major cellular fatty acids were C16:0, C19:0 cyclo ω7c, C17:0 cyclo ω7c and C17:1 ω6c. The dominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospholipid. Based on the genomic physiological and phenotypic characteristics, strain Bb-Pol-6 T was considered a novel species under the genus Robbsia, for which the name Robbsia betulipollinis sp. nov. was proposed. The type strain is Bb-Pol-6 T (= LMG 32774 T = DSM 114812 T).
Asunto(s)
Betula , Fosfolípidos , Fosfolípidos/química , Betula/genética , Filogenia , ARN Ribosómico 16S/genética , Técnicas de Tipificación Bacteriana , Ácidos Grasos/química , Polen/química , ADN , ADN Bacteriano/genética , Análisis de Secuencia de ADNRESUMEN
Phosphorus is a nutrient that is essential to nature and human life and has attracted attention because of its very limited reserves. Dwindling phosphorus reserves and soaring prices have made the recovery of phosphorus from waste biosolids even more urgent. Waste activated sludge, as the final destination of most of the phosphorus in human domestic and industrial water, has been considered as a reliable source of phosphorus recovery. The thermal treatment method of sewage sludge is currently a relatively environmentally friendly disposal method, which mainly includes incineration, pyrolysis and hydrothermal carbonization. This paper reviews the methods for the recovery of different forms of phosphorus (wet chemical, thermochemical and electrodialysis) from solid products obtained from different sludge thermal treatment methods (incinerated sewage sludge ash, pyrolysis of sewage sludge char and hydrochar) and the bioavailability of the recovered phosphorus products. Incineration of sewage sludge is currently the most established and effective method for recovering phosphorus from the thermal treatment products of sewage sludge. One of the wet chemical methods has been applied on a commercial scale and is expected to be further developed for future industrial applications. Pyrolysis and hydrothermal carbonation still have many research gaps in this field. Based on their principles and laboratory performance, both of them have the potential to recover phosphorus and should be further explored.
Asunto(s)
Fósforo , Aguas del Alcantarillado , Disponibilidad Biológica , Humanos , Incineración , PirólisisRESUMEN
BACKGROUND: Phosphorus deficiency is a major limiting factors for affecting crop production globally. To understand the genetic variation of phosphorus-deficiency-tolerance, a total of 15 seedling traits were evaluated among 707 Chinese wheat landraces under application of phosphorus (AP) and non-application of phosphorus (NP). A total of 18,594 single-nucleotide polymorphisms and 38,678 diversity arrays technology sequencing markers were used to detect marker-trait associations under AP and NP. RESULTS: Top ten genotypes with extremely tolerance and bottommost ten genotypes with extremely sensitivity were selected from 707 Chinese wheat landraces for future breeding and genetic analysis. A total of 55 significant markers (81 marker-trait associations) for 13 traits by both CMLM and SUPER method. These were distributed on chromosomes 1A, 1B, 2A, 2B, 2D, 3A, 4B, 5A, 5B, 6A, 6B, 6D, 7A and 7B. Considering the linkage disequilibrium decay distance, 25 and 12 quantitative trait loci (QTL) were detected under AP and NP, respectively (9 QTL were specific to NP). CONCLUSIONS: The extremely tolerant landraces could be used for breeding phosphorus-deficiency-tolerant cultivars. The QTL could be useful in wheat breeding through marker-assisted selection. Our findings provide new insight into the genetic analysis of P-deficiency-tolerance, and will be helpful for breeding P-deficiency-tolerant cultivars.
Asunto(s)
Variación Genética , Fósforo/deficiencia , Polimorfismo de Nucleótido Simple/genética , Sitios de Carácter Cuantitativo/genética , Triticum/genética , Estudios de Asociación Genética , Genotipo , Desequilibrio de Ligamiento , Fenotipo , Fitomejoramiento , Plantones/genética , Plantones/fisiología , Estrés Fisiológico , Triticum/fisiologíaRESUMEN
The co-delivery of photosensitizers with prodrugs sensitive to reactive oxygen species (ROS) for light-triggered ROS generation and cascaded prodrug activation has drawn tremendous attention. However, the absence of a feasible method to deliver the two components at a precise ratio has impaired the application potential. Herein, we report an efficient method to produce a nanosized platform for the delivery of an optimized ratio of the two components by the means of host-guest strategy for maximizing the combination therapy efficacy of cancer treatment. The key features of this host-guest strategy for the combination therapy are that the ratio between photosensitizer and ROS-sensitive prodrug can be easily tuned, near-infrared (NIR) irradiation can sensitize the photosensitizer and activate the paclitaxel prodrug for its release, and the accumulation process can be tracked by NIR imaging to maximize the efficacy of photodynamic and chemotherapy.