[Analysis of polysaccharide and lipid components of cinnamomi cortex by GC-MS].

OBJECTIVE: To study the polysaccharide and lipid components of Cinnamomi Cortex by GC-MS and provide experimental evidence for its exploitation. METHODS: The polysaccharide components of Cinnamomi Cortex were extracted,and hydrolyzed with trifluoroacetic acid (TFA) and followed by adding hydroxylamine hydrochloride, pyridine and acetic anhydride acetylation for reaction. After that, spectrometry of polysaccharides was measured by GC-MS. Lipid components were esterified and identified by GC-MS. RESULTS: 6 kinds of polysaccharide components were identified by GC-MS. D-glucofuranose accounted for 38.64%, which was the most proportion. 13 kinds of fatty acids were identified, accounted for 72.68% in total lipids. 5 kinds of unsaturated fatty acids accounted for 13.83% and 8 saturated fatty acids accounted for 58.85%. CONCLUSION: There are many kinds of polysaccharide and lipid components in Cinnamomi Cortex and GC-MS can analyze the polysaccharide and lipid components of Cinnamomi Cortex effectively.

[Optimal process of Flos Lonicerae in producing area--kill-enzyme torrefaction].

OBJECTIVE: To search for the optimal process of Flos Lonicerae in producing area, in order to offer scientific and applied process method for steadying the quality of Flos Lonicerae. METHODS: To summarize and use for reference the reported process method, searching for the relativity of different processes and quality. RESULTS: The content of Chlorogenic Acid in kill-enzyme torrefaction sample was 12. 8% higher than directly dried in the sun and 24.9% higher than dried in the shade. The content of Luteolin-7-glu in kill-enzyme torrefaction sample was 7.8% higher than the directly dried in the sun and 54.3% higher than dried in the shade. CONCLUSION: At present, kill-enzyme torrefaction is the optimal process of Flos Lonicerae in producing area It is an important technology in GAP large-scale of Flos Lonicerae.

[Somatic hybridization between carrot and Bupleurum scorzonerifolium and esteras isoenzyme analysis].

OBJECTIVE: To transfer the effective elements of Bupleurum scorzonerifolium into carrot, and provide theoretical data for the exploitation, improvement and selection of the germplasm of Chinese medicinal plants. METHOD: The protoplasta of Bupleurum scorzonerifolium irradiated by ultraviolet light (UV) at an intensity of 300 microW.(cm2)-1 for 0, 1, 2 min respectively were fused with those of carrot Fisch by PEG method. The regenerated clones, derived form a single fused cell, were examined for their hybrid nature by phenotype and Esterase isoenzyme analysis. RESULT: Nine clones were identified as the somatic hybrids between B. scorzonerifolium and carrot. CONCLUSION: This provides a firm foundation for the further analysis of the main active components saikosaponin of somatic hybrids and the screening out of high-medicine-content hybrid cell lines.