Recovery of nitrogen and phosphorus from livestock slurry with treatment technologies: A meta-analysis.

The livestock industry has developed rapidly in recent decades, but the improper treatment of livestock manure, especially slurry, causes environmental pollution. Treatment technologies are considered to be effective in alleviating nitrogen (N) and phosphorus (P) losses from livestock slurry. Here, we used published research data to conduct a meta-analysis of the recovery efficiencies of N and P of five mainstream treatment technologies, including ammonia stripping, air scrubbing, membrane filtration, microalgae cultivation and struvite crystallization. Additionally, the agronomic effects of the recovered products of these treatment technologies were evaluated. The results showed that all technologies exhibited clear recovery effects on N and P. The N recovery efficiencies ranged from 57% to 86%, and those of P ranged from 64% to 87%. Struvite crystallization was the most efficient treatment technology for both N and P recovery; moreover, the ammonia stripping and microalgae cultivation technologies were less efficient. The pH levels and temperatures are the main factors that influence ammonia stripping, struvite crystallization and microalgae cultivation, while membrane filtration and air scrubbing are mainly affected by the membrane types and properties. When the equal amount of N or P input to fields, the recovered products (ammonium sulfate and struvite crystals) may achieve a similar crop yield, relative to commercial N or P fertilizers. Our findings can provide deep suggestions and parameters for designing proper treatment technologies to reduce nutrient discharge from livestock slurry in regions with high livestock density and also for identifying the research gaps that should be paid more attention in the future.

A comparative light and electron microscopic analysis of microspore and tapetum development in fertile and cytoplasmic male sterile radish.

To gain further insight into the abortive stages and ultrastructural changes leading to pollen degeneration of a novel cytoplasmic male sterile radish 805A, we compared differences of cellular and subcellular structure of sterile anther with fertile anther by light and electron microscopy analysis. Two types of locule degeneration in sterile anther were detected, of which the time of degeneration occurred and completed was different. In type I, abnormality of pollen mother cells (PMCs) and tapetal cells, including condensation of cytoplasm and large vacuoles within tapetal cells, was shown at PMC stage. In type II, meiosis and early tetrad stage progressed normally except for large vacuoles that appeared in tapetal cells. Ultrastructural alterations of the cellular organization were observed in the type II locules, such as chromatin condensation at the periphery of the nucleus and degeneration of the karyotheca, compared with normal pollen development. The results suggested that the cytoplasmic male sterility anther degeneration was probably caused by dysfunctions of tapetum and vacuolation of tapetum, PMCs, and microspores. Thus, the identical factors, which induced CMS in the same cytoplasmic and nuclear genetic background, might affect development of tapetum and microspore at different stages during the cytoplasmic male sterile 805A anther development.