RESUMEN
BACKGROUND: To assess the benefits and harmful effects of Chinese herbal medicine (CHM) formulations in preventing anthracyclines (ANT)-induced cardiotoxicity. METHOD: The Cochrane Library, Pubmed and EMBASE databases were electronically searched for relevant randomized controlled trials (RCTs) published till December 2021 in English or Chinese-language, in addition to manual searches through the reference lists of the selected papers, and the Chinese Conference Papers Database. Data was extracted by 2 investigators independently. RESULT: Seventeen RCTs reporting 11 different CHMs were included in this meta-analysis. The use of CHM reduced the occurrence of clinical heart failure (RR 0.48, 95% CI 0.39 to 0.60, P < .01) compared to the control group. Data on subclinical heart failure in terms of LVEF values showed that CHM reduced the occurrence of subclinical heart failure (RR 0.47, 95% CI 0.35 to 0.62, P < .01) as well. CONCLUSION: CHM is an effective and safe cardioprotective intervention that can potentially prevent ANT-induced cardiotoxicity. However, due to the insufficient quality of the included trials, our results should be interpreted with cautious.
Asunto(s)
Medicamentos Herbarios Chinos , Insuficiencia Cardíaca , Neoplasias , Antraciclinas/efectos adversos , Antibióticos Antineoplásicos/uso terapéutico , Cardiotoxicidad/etiología , Cardiotoxicidad/prevención & control , Medicamentos Herbarios Chinos/uso terapéutico , Insuficiencia Cardíaca/inducido químicamente , Insuficiencia Cardíaca/tratamiento farmacológico , Insuficiencia Cardíaca/prevención & control , Humanos , Neoplasias/tratamiento farmacológico , Estudios ProspectivosRESUMEN
OBJECTIVE: To evaluate the effects of Celastrus Orbiculatus extracts (COE) on metastasis in hypoxia-induced hepatocellular carcinoma cells (HepG2) and to explore the underlying molecular mechanisms. METHODS: The effect of COE (160, 200 and 240 µ g/mL) on cell viability, scratch-wound, invasion and migration were studied by 3-4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2-H-tetrazolium bromide (MTT), scratch-wound and transwell assays, respectively. CoCl2 was used to establish a hypoxia model in vitro. Effects of COE on the expressions of E-cadherin, vimentin and N-cadherin were investigated with Western blot and immunofluorescence analysis, respectively. RESULTS: COE inhibited proliferation and metastasis of hypoxia-induced hepatocellular carcinoma cells in a dose-dependent manner (P<0.01). Furthermore, the expression of epithelial-mesenchymal transition (EMT) related markers were also remarkably suppressed in a dose-dependent manner (P<0.01). In addition, the upstream signaling pathways, including the hypoxia-inducible factor 1 α (Hif-1 α) and Twist1 were suppressed by COE. Additionally, the Hif-1 α inhibitor 3-5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1), potently suppressed cell invasion and migration as well as expression of EMT in hypoxia-induced HepG2 cells. Similarly, the combined treatment with COE and YC-1 showed a synergistic effect (P<0.01) compared with the treatment with COE or YC-1 alone in hypoxia-induced HepG2 cells. CONCLUSIONS: COE significantly inhibited the tumor metastasis and EMT by suppressing Hif-1 α/Twist1 signaling pathway in hypoxia-induced HepG2 cell. Thus, COE might have potential effect to inhibit the progression of HepG2 in the context of tumor hypoxia.
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Carcinoma Hepatocelular/tratamiento farmacológico , Carcinoma Hepatocelular/patología , Celastrus/química , Regulación hacia Abajo , Transición Epitelial-Mesenquimal , Neoplasias Hepáticas/tratamiento farmacológico , Neoplasias Hepáticas/patología , Extractos Vegetales/uso terapéutico , Biomarcadores de Tumor/metabolismo , Hipoxia de la Célula/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Forma de la Célula/efectos de los fármacos , Cobalto , Regulación hacia Abajo/efectos de los fármacos , Transición Epitelial-Mesenquimal/efectos de los fármacos , Células Hep G2 , Humanos , Invasividad Neoplásica , Metástasis de la Neoplasia , Proteínas de Neoplasias/metabolismo , Extractos Vegetales/farmacología , Transducción de Señal/efectos de los fármacosRESUMEN
<p><b>OBJECTIVE</b>To study the drug-guiding mechanism of Achyranthes bidentata from Sanmiao pill in arthritic rats.</p><p><b>METHOD</b>The rats were treated by Ferud's complet adjuvant and hot water -bath to establish rat adjuvant arthritis model, then the model rats were divided into three groups, model group, Sanmiao pill groups with A. bidentata. (18 g x kg(-1)) and without A. bidentata. (10 g x kg(-1)), respectively. The heart and foot joints were washed and homogenated to determine the berberine concentration by HPCL in different time after ig and the foot edema was tested by volume method. The pathological changes were observed and hemorheologic parameters were also tested.</p><p><b>RESULT</b>The berberine concentration of foot joint was significantly higher in 2, 4, 6 hour and 14 day in the rats with A. bidentata. The berberine concentration ratio of foot joint and heart was significantly higher in rats with A. bidentata. pharmacodynamic researches showed that A. bidentata could enhance the edema inhibition effect of Sanmiao pill. Hemorheologic researches showed that A. bidentata. could significantly improve the blood viscosity of model rats, the blood high shear viscosity, the blood low shear viscosity and the plasma viscosity were (6.47 +/- 0.57), (9.28 +/- 1.2), (1.94 +/- 0.19) mPa x s respectively.</p><p><b>CONCLUSION</b>A. bidentata. could facilitate the targeted tissue distribution of berberine. The effect was correlative with its blood viscosity improvement.</p>