RESUMEN
The localization of pituitary adenylate cyclase-activating polypeptide (PACAP) in the hypothalamus-pituitary system in rats was examined in light and electron microscopic immunocytochemistry using a specific antiserum to synthetic PACAP 1-38 (R0831). In light microscopic study, intensely PACAP-immunostained perikarya were observed in the supraoptic and paraventricular magnocellular nucleus in the hypothalamus. In the median eminence, many immunoreactive nerve fibers were observed in the internal layer, but a few immunoreactive terminals were noticed in the external layer. In the pituitary gland, numerous immunoreactive nerve fibers were observed in the posterior lobe. In the intermediate lobe, moderately immunostained cells were observed, but in the anterior lobe no immunostained cells were noticed. In electron microscopic study, PACAP-immunoreactivity was examined by avidin-biotin peroxidase complex method. In the perikarya of the supraoptic and paraventricular magnocellular nucleus, DAB-reaction products were distributed diffusely in the cytoplasmic matrix, frequently attaching to the rough-surfaced endoplasmic reticulum. In the nerve terminals of the posterior lobe, reaction products were observed among the secretory granules, but sometimes upon them. In the cells of the intermediate lobe, reaction products were also distributed in the cytoplasmic matrix.
Asunto(s)
Hipotálamo/química , Neuropéptidos/análisis , Hipófisis/química , Animales , Gránulos Citoplasmáticos/química , Retículo Endoplásmico/química , Hipotálamo/ultraestructura , Inmunohistoquímica , Masculino , Eminencia Media/química , Eminencia Media/ultraestructura , Microscopía Electrónica , Fibras Nerviosas/química , Núcleo Hipotalámico Paraventricular/química , Núcleo Hipotalámico Paraventricular/ultraestructura , Polipéptido Hipofisario Activador de la Adenilato-Ciclasa , Hipófisis/ultraestructura , Ratas , Ratas Wistar , Núcleo Supraóptico/química , Núcleo Supraóptico/ultraestructura , Distribución TisularRESUMEN
Aromatase-containing neurons were immunohistochemically examined in rat brains by using a polyclonal antibody against human placental antigen. The antibody recognizes cytochrome P-450 portion of aromatase, an enzyme converting androgen to estrogen. A large group of strongly immunoreactive cells was identified in the ventral pallidum, which extends caudally from the area surrounding the islands of Calleja. Other strongly or moderately stained cell groups were observed in the cerebral cortex, the amygdaloid area, the nucleus of the diagonal band, and the area anterior to the posterior commissure. Only a few stained cells were present in the medial preoptic region. These findings cast doubt upon the previous assumption, based on biochemical analysis of tissue samples, that the center of the aromatizing system is in the medial preoptic region. They indicate instead that most aromatase-containing neurons of rats lie within the ventral pallidum ventromedially adjacent to the preoptic area.
Asunto(s)
Antígenos/metabolismo , Aromatasa/metabolismo , Lóbulo Frontal/enzimología , Proteínas Gestacionales/inmunología , Animales , Femenino , Lóbulo Frontal/citología , Inmunohistoquímica , Masculino , Ratas , Ratas EndogámicasRESUMEN
The coexistence of substance P with neurotensin-like immunoreactivity in certain neurons of the hypothalamus were demonstrated by the double immunofluorescence method. Substance P and neurotensin-like immunoreactivity coexisted within single neurons of some hypothalamic areas such as the medial preoptic area, perifornical area, anterior hypothalamic area, lateral hypothalamic area, periventricular nucleus and posterior hypothalamic nucleus, although they did not coexist in the majority of immunoreactive cells.
Asunto(s)
Hipotálamo/análisis , Neuronas/análisis , Neuropéptidos/análisis , Neurotensina/análisis , Sustancia P/análisis , Animales , Técnica del Anticuerpo Fluorescente , Hipotálamo Anterior/análisis , Técnicas In Vitro , Masculino , Quiasma Óptico/análisis , Ratas , Núcleo Hipotalámico Ventromedial/análisisRESUMEN
The distribution of substance P- and enkephalin-like immunoreactivity in single cells were examined by the double immunofluorescence method. Substance P- and leucine-enkephalin-like compounds coexisted within individual neurons of some hypothalamic areas such as the medial preoptic area, anterior hypothalamic area, perifornical area, lateral hypothalamic area, premammillary nuclei and posterior hypothalamic nucleus, although they did not coexist in the majority of immunoreactive cells.
Asunto(s)
Encefalinas/metabolismo , Hipotálamo/metabolismo , Péptidos/metabolismo , Sustancia P/metabolismo , Animales , Hipotálamo Medio/metabolismo , Inmunohistoquímica/métodos , Masculino , Ratas , Coloración y EtiquetadoRESUMEN
The origins of neuropeptide Y-like immunoreactive (NPYI) fibers in the paraventricular and dorsomedial hypothalamic nuclei of the rat were examined using immunohistochemistry. Destruction of the arcuate nucleus resulted in a marked decrease of NPYI fibers ipsilaterally in these nuclei, suggesting that most of NPYI fibers in these nuclei originate from NPYI neurons in the arcuate nucleus. These NPYI systems did not contain noradrenalin.