RESUMEN
Fifty four Trichoderma strains were isolated from soil samples collected from garlic and onion crops in eight different sites in Brazil and were identified using phylogenetic analysis based on combined ITS region, tef1-α, cal, act and rpb2 sequences. The genetic variability of the recovered Trichoderma species was analysed by AFLP and their phenotypic variability determined using MALDI-TOF. The strain clusters from both typing techniques coincided with the taxonomic determinations made from phylogenetic analysis. The phylogenetic analysis showed the occurrence of Trichoderma asperellum, Trichoderma asperelloides, Trichoderma afroharzianum, Trichoderma hamatum, Trichoderma lentiforme, Trichoderma koningiopsis, Trichoderma longibrachiatum and Trichoderma erinaceum, in the soil samples. We also identified and describe two new Trichoderma species, both in the harzianum clade of section Pachybasium, which we have named Trichoderma azevedoi sp. nov. and Trichoderma peberdyi sp. nov. The examined strains of both T. azevedoi (three strains) and T. peberdyi (12 strains) display significant genotypic and phenotypic variability, but form monophyletic clades with strong bootstrap and posterior probability support and are morphologically distinct from their respective most closely related species.
Asunto(s)
Ajo/microbiología , Cebollas/microbiología , Microbiología del Suelo , Trichoderma/clasificación , Trichoderma/aislamiento & purificación , Análisis del Polimorfismo de Longitud de Fragmentos Amplificados , Biodiversidad , Brasil , ADN de Hongos/análisis , ADN de Hongos/genética , Técnicas de Tipificación Micológica/métodos , Filogenia , Análisis de Secuencia de ADN/métodos , Especificidad de la Especie , Trichoderma/citología , Trichoderma/genéticaRESUMEN
Os autores analisam o conceito de doença e de doente, suas implicações no tratamento médico, na assistência médica e na adequação do tratamento homeopáticos aos pacientes que se tratam em Centros de Saúde. Estão executando um projeto de atendimento a pacientes no Centro de Saúde da Barra Funda em São Paulo, onde se propõem a analisar a eficiência terapêutica homeopática, através dos resultados do tratamento dos doentes, do tempo gasto nas consultas, das intercorrências durante o tratamento e o custo medicamentoso. O trabalho está em andamento e somente alguns dados preliminares foram avaliados. Este trabalho constitui uma contribuição para a estruturação do atendimento homeopático na rede pública. (au)
The authors analyze the notions of disease/sick person, their implications for medical treatment, medical care and the suitability of homeopathic treatment for patients cared at primary healthcare services. The authors are carrying out a project involving care delivery to patients at Health Care Center of Barra Funda, São Paulo, to analyze the efficiency of homeopathic treatment based on the outcomes of treatment, visit duration, intercurrent problems and cost. This project is in progress and only some preliminary data were evaluated. The present study represents a contribution to the implementation of homeopathic care within the public healthcare system. (au)