Chronic exposure to lipopolysaccharides as an in vitro model to simulate the impaired odontogenic potential of dental pulp cells under pulpitis conditions.

BACKGROUND: Simulating a bacterial-induced pulpitis environment in vitro may contribute to exploring mechanisms and bioactive molecules to counteract these adverse effects. OBJECTIVE: To investigate the chronic exposure of human dental pulp cells (HDPCs) to lipopolysaccharides (LPS) aiming to establish a cell culture protocol to simulate the impaired odontogenic potential under pulpitis conditions. METHODOLOGY: HDPCs were isolated from four healthy molars of different donors and seeded in culture plates in a growth medium. After 24 h, the medium was changed to an odontogenic differentiation medium (DM) supplemented or not with E. coli LPS (0 - control, 0.1, 1, or 10 µg/mL) (n=8). The medium was renewed every two days for up to seven days, then replaced with LPS-free DM for up to 21 days. The activation of NF-κB and F-actin expression were assessed (immunofluorescence) after one and seven days. On day 7, cells were evaluated for both the gene expression (RT-qPCR) of odontogenic markers (COL1A1, ALPL, DSPP, and DMP1) and cytokines (TNF, IL1B, IL8, and IL6) and the production of reactive nitrogen (Griess) and oxygen species (Carboxy-H2DCFDA). Cell viability (alamarBlue) was evaluated weekly, and mineralization was assessed (Alizarin Red) at 14 and 21 days. Data were analyzed with ANOVA and post-hoc tests (α=5%). RESULTS: After one and seven days of exposure to LPS, NF-κB was activated in a dose-dependent fashion. LPS at 1 and 10 µg/mL concentrations down-regulated the gene expression of odontogenic markers and up-regulated cytokines. LPS at 10 µg/mL increased both the production of reactive nitrogen and oxygen species. LPS decreased cell viability seven days after the end of exposure. LPS at 1 and 10 µg/mL decreased hDPCs mineralization in a dose-dependent fashion. CONCLUSION: The exposure to 10 µg/mL LPS for seven days creates an inflammatory environment that is able to impair by more than half the odontogenic potential of HDPCs in vitro, simulating a pulpitis-like condition.

Chronic exposure to lipopolysaccharides as an in vitro model to simulate the impaired odontogenic potential of dental pulp cells under pulpitis conditions

Abstract Simulating a bacterial-induced pulpitis environment in vitro may contribute to exploring mechanisms and bioactive molecules to counteract these adverse effects. Objective To investigate the chronic exposure of human dental pulp cells (HDPCs) to lipopolysaccharides (LPS) aiming to establish a cell culture protocol to simulate the impaired odontogenic potential under pulpitis conditions. Methodology HDPCs were isolated from four healthy molars of different donors and seeded in culture plates in a growth medium. After 24 h, the medium was changed to an odontogenic differentiation medium (DM) supplemented or not with E. coli LPS (0 - control, 0.1, 1, or 10 µg/mL) (n=8). The medium was renewed every two days for up to seven days, then replaced with LPS-free DM for up to 21 days. The activation of NF-κB and F-actin expression were assessed (immunofluorescence) after one and seven days. On day 7, cells were evaluated for both the gene expression (RT-qPCR) of odontogenic markers (COL1A1, ALPL, DSPP, and DMP1) and cytokines (TNF, IL1B, IL8, and IL6) and the production of reactive nitrogen (Griess) and oxygen species (Carboxy-H2DCFDA). Cell viability (alamarBlue) was evaluated weekly, and mineralization was assessed (Alizarin Red) at 14 and 21 days. Data were analyzed with ANOVA and post-hoc tests (α=5%). Results After one and seven days of exposure to LPS, NF-κB was activated in a dose-dependent fashion. LPS at 1 and 10 µg/mL concentrations down-regulated the gene expression of odontogenic markers and up-regulated cytokines. LPS at 10 µg/mL increased both the production of reactive nitrogen and oxygen species. LPS decreased cell viability seven days after the end of exposure. LPS at 1 and 10 µg/mL decreased hDPCs mineralization in a dose-dependent fashion. Conclusion The exposure to 10 µg/mL LPS for seven days creates an inflammatory environment that is able to impair by more than half the odontogenic potential of HDPCs in vitro, simulating a pulpitis-like condition.

Positive influence of simvastatin used as adjuvant agent for cavity lining.

OBJECTIVES: To assess the biological, antimicrobial, and mechanical effects of the treatment of deep dentin with simvastatin (SV) before application of a glass-ionomer cement (GIC). MATERIALS AND METHODS: Dentin discs were adapted to artificial pulp chambers and SV (2.5 or 1.0 mg/mL) was applied to the occlusal surface, either previously conditioned or not with EDTA (±EDTA). The extracts (culture medium + SV that diffused through dentin) was obtained and then applied to cultured odontoblast-like MDPC-23 cells. Cell viability, alkaline phosphatase (ALP) activity, and mineralization nodule (MN) deposition were evaluated. Untreated discs were used as control. The antibacterial activity of SV (2.5 or 1.0 mg/mL) against Streptococcus mutans and Lactobacillus acidophilus, as well as the bond strength of GIC to dentin in the presence of SV 2.5 mg/mL (±EDTA) were also assessed. The data were analyzed by ANOVA/Tukey tests (α = 5%). RESULTS: EDTA + SV 2.5 mg/mL significantly enhanced the ALP activity and MN deposition in comparison with the control, without changing in the cell viability (p < 0.05). The association EDTA + SV 2.5 mg/mL + GIC determined the highest ALP and MN values (p < 0.05). SV presented intense antimicrobial activity, and the EDTA dentin conditioning followed by SV application increased bond strength values compared with SV treatment alone (p < 0.05). CONCLUSION: SV presents antimicrobial activity and diffuses across conditioned dentin to biostimulate odontoblast-like pulp cells. CLINICAL SIGNIFICANCE: The use of SV as adjuvant agent for indirect pulp capping may biostimulate pulp cells thus preserving vitality and function of the pulp-dentin complex.

Epithelial cell-enhanced metabolism by low-level laser therapy and epidermal growth factor.

Reepithelialization and wound closure are the desired outcome for several ulcerative conditions. Such resolution reduces the possibility of wound contamination and maintenance of the injury and improves the reestablishment of tissue morphology and functions. Investigators are seeking adjuvant therapies that can accelerate wound healing and are developing new strategies for clinical applications. This study compared the effects of epidermal growth factor (EGF) application and low-level laser therapy (LLLT) on cultured epithelial cells. Cells were seeded in 24-well plates. After a 24-h incubation, the epithelial cells were either treated with EGF (100 µM in serum-free DMEM for 72 h) or subjected to LLLT (780 nm, 25 mW, 0.5, 1.5, and 3 J/cm2) by three applications every 24 h. Seventy-two hours after cells were treated with EGF or LLLT, cell migration, viability, proliferation, and collagen synthesis were assessed. Cells treated with EGF showed increased cell viability, proliferation, and collagen synthesis compared with those cells that received no treatment. LLLT enhanced cell migration; however, no significant effects of laser irradiation on other cell functions were observed. Comparison of both therapies demonstrated that EGF and LLLT enhanced specific epithelial cell activities related to wound healing.

Photobiomodulation in the Metabolism of Lipopolysaccharides-exposed Epithelial Cells and Gingival Fibroblasts.

This study assessed the effects of photobiomodulation (PBM) to cells previously exposed to lipopolysaccharides (LPS). Human gingival fibroblasts (HGF) and epithelial cells (HaCaT) were seeded in wells of 24-well plates containing complete culture medium (DMEM). After 24 h, the DMEM was replaced by serum-free DMEM, and cells were exposed to LPS of Escherichia coli (E. coli) (10 µg mL-1 ) for 24, 48, and 72 h. The cells were subjected to specific parameters of phototherapy (PT) (LASERTable-InGaAsP-780 ± 3 nm, 25 mW, 3 J cm-2 ). Cell proliferation (alamarBlue® ), viability (Trypan Blue) and synthesis of CCL2 (ELISA) were evaluated. Data were statistically analyzed by the Kruskal-Wallis and Mann-Whitney test (α = 5%). Proliferation and viability of both cell lines decreased after LPS treatment at 48 and 72 h. Enhanced synthesis of CCL2 by gingival fibroblasts occurred at 24 h, while epithelial cells increased synthesis of this chemokine at 48 and 72 h. PBM enhanced cell proliferation and viability in a time-dependent manner for both cell lines exposed or not to LPS, while synthesis of CCL2 by cells exposed to PT decreased over time. PBM caused biomodulatory effects on gingival fibroblasts and epithelial cells previously treated with LPS. These effects may decrease tissue inflammatory response and accelerate wound healing of oral mucosal tissue.

Bioestimulação de células pulpares humanas expostas a sinvastatina / Biostimulation of human pulp cells exposed to simvastatin

O objetivo desse estudo foi avaliar o potencial bioativo da sinvastatina (SV), aplicada por diferentes períodos sobre células da polpa dental humana (HDPCs). Para isto, HDPCs em 80% de confluência (n=6) foram tratadas com meio osteogênico suplementado com 0,01 µM de SV pelos períodos de 24 h, 72 h ou continuamente por até 21 dias. No controle negativo, as células foram mantidas em meio osteogênico. A viabilidade celular (MTT) foi avaliada em períodos de 1, 3, 7, 14 e 21 dias, e a deposição de matriz mineralizada (alizarin red) após 14 e 21 dias de cultivo celular. Os dados foram submetidos aos testes ANOVA e Tukey (α=5%). Foi observado que nos períodos de 1, 3, 7 e 14 dias não houve diferença significativa na viabilidade das células submetidas aos tratamentos com SV em comparação ao controle (p<0,05); no entanto, redução tardia foi observada aos 21 dias para as células tratadas com SV por 72 h ou de modo contínuo (p<0,05). Em contrapartida, aumento na deposição de matriz mineralizada foi observado para o tratamento contínuo com SV aos 21 dias, quando comparado ao controle (p<0,05). Foi possível concluir que o tratamento contínuo de células pulpares humanas com 0,01µM de SV foi capaz de bioestimular a deposição de matriz mineralizada in vitro.

The objective of this study was to evaluate the bioactive potential of simvastatin (SV), applied during different periods on human dental pulp cells (HDPCs). For this, HDPCs at 80% confluency (n = 6) were treated with osteogenic medium supplemented with 0.01 µM SV for periods of 24 h, 72 h or continuously up to 21 days. In the negative control group, the cells were cultivated in osteogenic medium. The cell viability (MTT) was evaluated after 1, 3, 7, 14 and 21 days, and the mineralized matrix deposition (alizarin red) was assessed at 14 and 21 days of cell culture. Data were submitted to ANOVA and Tukey's test (α=5%). No significant difference in cell viability was observed at 1, 3, 7 and 14 days for the cells exposed to SV compared to negative control (p<0.05); however, significant reduction was observed at 21 days for cells treated with SV during 72 h or continuously (p<0.05). On the other hand, increase in mineralized matrix deposition at 21 days was observed for cells treated continuously with SV when compared to control (p<0.05). It was possible to conclude that the continuous treatment of human pulp cells with 0.01 µM of SV was able to biostimulate mineralized matrix deposition in vitro.

Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals.

This study evaluated the effects of low-level laser therapy (LLLT) and epidermal growth factor (EGF) on fibroblasts obtained from young and elderly individuals. Gingival fibroblasts from young (Y) and elderly (E) individuals were seeded in wells of 24-well plates with Dulbecco's modified Eagle's medium (DMEM) containing 10 % of fetal bovine serum (FBS). After 24 h, the cells were irradiated (LASERTable-InGaAsP-780 ± 3 nm, 25 mW, 3 J/cm2) or exposed to EGF (100 µM). After 72 h, cells were evaluated for viability, migration, collagen and vascular endothelial growth factor (VEGF) synthesis, and gene expression of growth factors. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (α = 5 %). Y and E fibroblasts irradiated with laser or exposed to EGF showed increased viability and collagen synthesis. Enhanced cell migration was observed for Y fibroblasts after both treatments, whereas only the LLLT stimulated migration of E cells. VEGF synthesis was higher for Y and E cells exposed to EGF, while this synthesis was reduced when E fibroblasts were irradiated. Increased gene expression of VEGF was observed only for Y and E fibroblasts treated with LLLT. Regardless of a patient's age, the LLLT and EGF applications can biostimulate gingival fibroblast functions involved in tissue repair.

Avaliação Clínica e Microbiológica do Tratamento da EstomatiteProtética com Tintura de Schinus terebinthifolius Raddi (Aroeira) / Clinical and Microbiological Evaluation of the Treatment of Denture Stomatitis withSchinus terebinthifolius Raddi (Aroeira) Tincture

Objeti vo: Avaliar a efi cácia clínica da ti ntura da aroeira notratamento da estomati te protéti ca.Método: Foram selecionados 18 pacientes usuários de prótesesremovíveis com diagnósti co clínico para estomati te protéti cati po II e presença de candidose associada à prótese, constatadosa parti r de um exame clínico e micológico. Os pacientes foramdistribuídos em dois grupos: GT (grupo teste) - tratamentocom a ti ntura da aroeira; GC (grupo controle), tratamento comnistati na. Todos os pacientes foram orientados a higienizara prótese com escova e denti frício e, em seguida, aplicar oproduto na mucosa palati na e na superfí cie da prótese 3 vezesao dia, durante 15 dias consecuti vos, remover a prótese à noitee armazená-la em um recipiente com água. No 15º dia de uso,foi realizado um novo exame clínico e micológico para avaliara efi cácia do tratamento. Os dados foram analisados com ostestes Wilcoxon e Mann-Whitney com nível de signfi cância de5%.Resultados: Observou-se eliminação do processo infl amatórioe da infecção por Candida spp. em 66,7% e 77,8% dos casos,respecti vamente, para GT. Já para GC, a eliminação doprocesso infl amatório e da infecção fúngica ocorreu em 77,8%e 88,9% dos casos, respecti vamente. Estes resultados foramestati sti camente signifi cantes (Wilcoxon p=0,01). Não foiobservada diferença estatí sti camente signifi cante entre os doistratamentos (Mann-Whitney p>0,05). A infecção fúngica foidiagnosti cada apenas na prótese em todos os casos, sendo a C.albicans o microorganismo mais prevalente, estando presenteem 94,4% dos casos.Conclusão: O tratamento com a ti ntura da aroeira foi efi caz notratamento da estomati te protéti ca, promovendo remissão doprocesso infl amatório e da infecção por Candida spp...

Objecti ve: To evaluate the clinical effi cacy of Schinusterebinthifolius Raddi (aroeira) ti ncture in the treatment ofdenture stomati ti s.Method: Eighteen removable denture wearers with clinicaldiagnosis of type II denture stomati ti s and presence ofcandidosis associated to the denture use, as confi rmed byclinical and mycological examinati ons, were selected forthe study. The pati ents were allocated to two groups: TG(test group) - treatment with Schinus terebinthifolius Raddi(aroeira) ti ncture; CG (control group), treatment with nystati n.All pati ents were instructed to clean the dentures withtoothbrush and denti frice, and then apply the product on thepalatal mucosa and on denture surface 3 ti mes a day, during15 consecuti ve days, removing the denture at bedti me andkeeping it in a receptacle with water. At the 15th day of use, theclinical and mycological examinati ons were redone to evaluatetreatment effi cacy. Data were analyzed stati sti cally by Wilcoxonand Mann-Whitney tests at 5% signifi cance level.Results: The infl ammatory process and Candida spp. infecti onwere eliminated in 66.7% and 77.8% of the cases, respecti vely,in TG. In CG, eliminati on of the infl ammatory process andfungal infecti on occurred in 77.8% and 88.9% of the cases,respecti vely. These results were stati sti cally signifi cant (p=0.01).There was no stati sti cally signifi cant diff erence between thetreatments (p>0.05). In all cases, fungal infecti on was detectedonly on the denture, and C. albicans was the most prevalentmicroorganism, being present in 94.4% of the cases.Conclusion: The treatment with Schinus terebinthifolius Raddi(aroeira) ti ncture was eff ecti ve in the treatment of denturestomati ti s, promoti ng remission of the infl ammatory processand Candida spp infecti on...

Avaliação da eficácia da descontaminação de escovas dentárias pelo uso do spray de óleo essencial da eugenia uniflora l. (Pitanga) / Evaluation of the effectiveness of the decontamination of toothbrushes by the use of the spray of essential oil of eugenia uniflora l. (Pitanga)

Objetivou-se avaliar a eficácia do uso de spray de óleo essencial da Eugenia uniflora L. (Pitanga) na descontaminação de escovas dentárias. Foi realizado um ensaio clínico cruzado duplo cego, com uma amostra de 28 universitários entre 19 e 25 anos de idade, de ambos os gêneros, que não utilizavam antibióticos ou anti-sépticos e autorizaram sua participação por meio da assinatura do Termo de Consentimento Livre e Esclarecido. Os participantes usaram três sprays pelo período padronizado de uma semana: spray teste (pitanga a 2%, água destilada e Tween-80), spray controle positivo (clorexidina a 2%) e spray controle negativo (água destilada e Tween-80). A cada semana, foi disponibilizado um “kit” contendo escova dental com capa, creme dental, e um dos três sprays, tendo um intervalo de uma semana entre o uso destes. Avaliou-se o grau de contaminação bacteriana das escovas pelo S. mutans, depois do uso de cada spray por uma semana, sendo semeadas as diluições de 10-3 do soro fisiológico, onde as escovas foram submersas, no meio de cultura Ágar Mitis Salivarius Bacitracina. Após a semeadura, as placas foram incubadas em estufa a 37ºC por 48 horas em microaerofilia e feita a contagem de UFC/mL. As médias de UFC/mL foram: spray teste = 1968,07, controle negativo = 4867,82 e controle positivo = 337,93. Foram observadas diferenças significativas ao nível de 1% (p = 0,01) ao Teste t de Student entre as médias para todos os grupos. Concluiu-se que o spray testado foi eficaz na descontaminação das escovas dentárias.

The study aimed to evaluate the effectiveness of the use of spray of essential oil of Eugenia uniflora L. (Pitanga) in vivo in the decontamination of toothbrushes. It was realized a cross-over random double-blind clinical assay, with a sample of 28 college students between 19 and 25 years old, of both sex, that did not use anti-septic or antibiotics and had authorized its participation by the signature of the Term of Free and Clarified Assent. The participants used three sprays for the standardized period of one week: spray test (2% of pitanga, distilled water and Tween-80), spray positive control (2% of chlorhexidine) and spray negative control (distilled waterand Tween-80). Every week, a “kit” was available containing toothbrush with layer for protection, dental cream, and one of three sprays, having an interval of one week between the use of these. The level of bacterial contamination of the toothbrushes by the S. mutans was evaluated after the use of each spray for one week, being seeded the dilutions of 10-3 of the physiological saline, where the toothbrushes were submerged, on Mitis Salivarius-Bacitracina Agar (DIFCO®). After sowing, the plates were incubated in bacteriological greenhouse at 37ºC for 48 hours in microaerophily and it was made the colony count of CFU/ml. The averages of CFU/ml were: spray test = 1968,07, negative control = 4867,82 and positive control = 337,93. Significant differences to the level of 1% (p = 0.01) to Test t of Student were observed between the averages for all the groups. It was concluded that the spray tested was efficient in the decontamination of the toothbrushes.

Atividade antibacteriana de tinturas de plantas tropicais sobre microorganismos da cavidade bucal / Antibacterial activity of tinctures of tropical plants against microorganisms of the buccal socket

O objetivo deste trabalho foi avaliar in vitro a atividade antibacteriana das tinturas das folhas do cajá,da casca do limão e das folhas do jenipapo sobre microorganismos da cavidade bucal. As tinturas foram preparadas pela técnica da maceração obtendo-se uma concentração de 20%. As linhagens bacterianas selecionadas foram: S. aureus (ATCC 25923), S. mutans (ATCC 2575), S. sobrinus (ATCC 27609), e L. casei (ATCC 7469). A clorexidina 0,12% foi utilizada como controle positivo. Determinou-se a Concentração Inibitória Mínima (CIM) em meio de cultura Agar Mueller Hinton. As placas de Petri foram semeadas pela técnica da inundação sendo em seguida realizadas sete perfurações de 6mm de diâmetro, onde foram inoculados 50ìl das tinturas na forma pura (1:0) e diluídas de 1:1 até 1:32. As placas foram incubadas a 37°C em microaerofilia por 24 horas. Os resultados mostraram que as tinturas de limão e cajá foram efetivas sobre o S. sobrinus, até as concentrações de 2,5% e 0,3125%, e sobre o S. aureus até 10% e 1,25%, respectivamente; a tintura do jenipapo foi efetiva apenas sobre o S. sobrinus, apresentando ação até 10%. O S. mutans e o L. casei não apresentaram crescimento inibido frente as tinturas avaliadas. Conclui-se que o S. sobrinus e o S. aureus foram os microorganismos mais susceptíveis; as tinturas de cajá e a de limão foram as que obtiveram os melhores resultados; as tinturas não apresentaram atividade antibacteriana sobre o S. mutans e o L. casei

The objective of this work was evaluated in vitro the antibacterial activity of the tinctures from the leaf of cajá, rind of lemon and leaf of jenipapo against microorganisms of the buccal socket. The bacterial sorts selected were: S. aureus (ATCC 25923), S. mutans (ATCC 2575), S. sobrinus (ATCC 27609), and L. casei (ATCC 7469). The clorexidine to 0,12% it was utilized as a positive control. It was determined the Minimum Inhibitory Concentration (MIC) in culture medium Agar Mueller Hinton. The plates of Petri were sown by the technique of flooding being, after that, realized seven perforations of 6mm to diameter, where had been inoculate 50ì l of the tinctures in the pure form (1:0) and diluted by 1:1 until 1:32. The plates of Petri were incubates to 37°C in microaerofily by 24 hours. The results shown that the tinctures of lemon and cajá were effectiveness against the S. sobrinus, until the concentration of 2,5% and 0,3125%, and against the S. aureus, until 10% and 1,25%, respectively; the tincture from jenipapo was effective just against the S. sobrinus, presenting action until 10%. The S. mutans and the L. casei didn’t presented inhibition of growth front the tinctures evaluated. It was concluded that the S. sobrinus and the S. aureus were the microorganisms most susceptible; the tinctures from cajá and limão were the one that got the best results; the tinctures don’t presented antibacterial activity against the S. mutans and the L. casei

Atividade antimicrobiana in vitro da eugeniauniflora L. (Pitanga) sobre bactérias cariogênicas / Antibacterial activity in vitro of eugenia uniflora L. (pitanga) against cariogenic bacteria

Objetivo: Verificar a ação antibacteriana in vitro da Eugeniauniflora L. (Pitanga) frente a bactérias cariogênicas. Materiale Métodos: Avaliaram-se: (A) extrato hidroalcoólico do frutomaduro, (B) extrato hidroalcoólico do fruto verde, (C) infusoda folha fresca, (D) óleo essencial da folha fresca e (E)solução de clorexidina a 0,2% (controle positivo). Utilizaram se linhagens bacterianas de Streptococcus mutans (ATCC25175), Streptococcus sanguis (ATCC 15300),Streptococcus salivarius (ATCC 7073), Streptococcus mitis(ATCC 903) e Streptococcus oralis (ATCC 10557).Determinou-se a Concentração Inibitória Mínima (CIM) em meiosólido Ágar Müeller Hinton (DIFCO®), pela técnica dos poços,nas concentrações de 10% e diluições desta de 1:1 até1:32. Resultados: A CIM encontrada para A, B e C foi, respectivamente,2,5%, 5% e 2,5% sobre S. mutans; 0,3125%, 5% e0,15625%, sobre S. sanguis; 10%, 10% e 5%, sobre S.mitis; 2,5%, 5% e 5%, sobre S. salivarius; 0,15625%, 0,625%e 0,15625%, sobre S. oralis. O óleo essencial da folha dapitanga não demonstrou atividade antibacteriana. Conclusão:os extratos hidroalcoólicos do fruto verde e maduro, bemcomo o infuso da folha fresca da pitanga apresentaramatividade antibacteriana sobre as bactérias testadas; o óleoessencial da folha da pitanga não apresentou açãoantibacteriana nas concentrações igual e inferiores a 10%;o infuso da folha fresca da pitanga obteve os maiores valoresde CIM; o S. oralis foi o microrganismo mais sensível, enquantoque o S. mitis se mostrou o mais resistente.

Objective: To verify the antibacterial activity in vitro of Eugeniauniflora L. (pitanga) against cariogenic bacteria. Materialand Methods: It was evaluated: (A) Ethanol extract of themature fruit, (B) Ethanol extract of the green fruit, (C) liquidextract of the cool leaf, (D) essential oil of the cool leaf and(E) 0,2% of chlorexidine solution (positive control). It wasused strain of Streptococcus mutans (ATCC 25175),Streptococcus sanguis (ATCC 15300), Streptococcussalivarius (ATCC 7073), Streptococcus mitis (ATCC 903),Streptococcus oralis (ATCC 10557). It was determined MinimalInhibitory Concentration (MIC) in solid medium Müeller HintonÁgar (DIFCO®), for the technique of the wells in theconcentrations of 10% and its dilutions of 1:1 until 1:32.Results: The MIC found for A, B and C were, respectively,2.5%, 5% and 2.5% for S. mutans; 0.3125%, 5% and0.15625%, for S. sanguis; 10%, 10% and 5%, for S. mitis;2.5%, 5% and 5%, for S. salivarius; 0.15625%, 0.625% and0.15625%, for S. oralis. The data gotten for the essential oilof the leaf of Eugenia uniflora L. didn’t demonstrate antibacterialactivity. Conclusions: the Ethanol extracts produced from thegreen and mature fruits, as well as of liquid extract of thecool leaf of pitanga presented antibacterial activity on thestrain plaque bacteria former tested; the essential oil of theleaf of pitanga didn’t present antibacterial activity inconcentrations equal and smaller than 10%; liquid extract ofthe cool leaf of pitanga was the extration form that gotgreaters values of MIC; the S. oralis was the bacteria mostsensitive, while the S. mitis showed to be the most resistant.

Atividade antibacteriana in vitro da tintura de aroeira (Schinusterebinthifolius) na descontaminação de escovas dentaiscontaminadas pelo s. mutans / Antibacterial activity in vitro of the tincture of aroeira (schinus terebinthifolius) in the decontamination of dental brushs contamined by S. mutans

Objetivo: Avaliar a atividade antibacteriana in vitro da tintura dacasca da aroeira a 20 porcento sobre o Streptococcus mutans e observara eficácia deste fitoterápico na descontaminação de escovasdentais in vitro contaminadas com este microorganismo.Método: Determinou-se a atividade antibacteriana in vitro sobre alinhagem padrão de S. mutans (ATCC 2575), através da técnica dedifusão em ágar para determinação da Diluição Inibitória Máxima(DIM). As placas de Petri contendo o meio de cultura Ágar MuellerHinton foram semeadas pela técnica da inundação, sendo realizados7 poços, de 6mm de diâmetro, onde foram inoculados 50ul da tinturade aroeira nas formas pura (1:0) e diluída de 1:1 até 1:32. Comocontrole positivo utilizou-se a clorexidina a 0,12 por cento. Em seguidaavaliou-se a descontaminação das escovas dentais, in vitro. Asescovas foram contaminadas em meio de cultura BHI contendo oS. mutans, através da imersão das cerdas por 1min, sendo realizadaa descontaminação com as seguintes soluções sob a forma despray: tintura da aroeira, clorexidinha 0,12 porcento (controle positivo) eágua destilada (controle negativo). Para verificação da inibição docrescimento, observou-se a turvação do meio líquido, apósincubação por 24h em microaerofilia, e realizou-se a semeadurana concentração de 10-3 em meio de cultura Mitis-SalivariusBacitracina para contagem das UFC/ml.Resultados: Os resultados referentes à atividade antibacterianasobre o S. mutans, demonstraram ação da tintura de aroeira até adiluição de 1:8, e da clorexidina até a diluição de 1:32. Com relaçãoà descontaminação das escovas dentárias, verificou-se que oinóculo da solução de clorexidina a 0,12% foi único totalmentelímpido após as 24 horas. Ao realizar-se a semeadura para contagemdas UFC/ml, observou-se que a clorexidina apresentou UFC/ml iguala zero, a tintura...

Atividade antibacteriana in vitro da tintura de aroeira (Schinusterebinthifolius) na descontaminação de escovas dentaiscontaminadas pelo s. mutans / Antibacterial activity in vitro of the tincture of aroeira (schinus terebinthifolius) in the decontamination of dental brushs contamined by S. mutans

Objetivo: Avaliar a atividade antibacteriana in vitro da tintura dacasca da aroeira a 20 porcento sobre o Streptococcus mutans e observara eficácia deste fitoterápico na descontaminação de escovasdentais in vitro contaminadas com este microorganismo.Método: Determinou-se a atividade antibacteriana in vitro sobre alinhagem padrão de S. mutans (ATCC 2575), através da técnica dedifusão em ágar para determinação da Diluição Inibitória Máxima(DIM). As placas de Petri contendo o meio de cultura icgar MuellerHinton foram semeadas pela técnica da inundação, sendo realizados7 poços, de 6mm de diâmetro, onde foram inoculados 50ul da tinturade aroeira nas formas pura (1:0) e diluída de 1:1 até 1:32. Comocontrole positivo utilizou-se a clorexidina a 0,12 por cento. Em seguidaavaliou-se a descontaminação das escovas dentais, in vitro. Asescovas foram contaminadas em meio de cultura BHI contendo oS. mutans, através da imersão das cerdas por 1min, sendo realizadaa descontaminação com as seguintes soluções sob a forma despray: tintura da aroeira, clorexidinha 0,12 porcento (controle positivo) eágua destilada (controle negativo). Para verificação da inibição docrescimento, observou-se a turvação do meio líquido, apósincubação por 24h em microaerofilia, e realizou-se a semeadurana concentração de 10-3 em meio de cultura Mitis-SalivariusBacitracina para contagem das UFC/ml.Resultados: Os resultados referentes à atividade antibacterianasobre o S. mutans, demonstraram ação da tintura de aroeira até adiluição de 1:8, e da clorexidina até a diluição de 1:32. Com relaçãoà descontaminação das escovas dentárias, verificou-se que oinóculo da solução de clorexidina a 0,12% foi único totalmentelímpido após as 24 horas. Ao realizar-se a semeadura para contagemdas UFC/ml, observou-se que a clorexidina apresentou UFC/ml iguala zero, a tintura...

Susceptibilidade in vitro de bactérias bucais a tinturas fitoterápicas / Susceptibility in vitro of the buccal bacterias to phytotherapics tinctures

O Brasil dispõe de uma diversidade de substâncias naturais com propriedades terapêuticas bastante difundidas dentro da Odontologia Preventiva. Neste trabalho, objetivou-se avaliar a atividade antibacteriana das tinturas de jucá, aroeira, gengibre, alfavaca, própolis, romã e hortelã da folha graúda, sobre as linhagens de S. aureus (ATCC 25923), S. mutans (ATCC 2575), S. sobrinus (ATCC 27609), S. mitis (ATCC 9811), S. sanguis (ATCC 10557) e L. casei (ATCC 7469), utilizando-se a clorexldina 0,12% como controle positivo. Determinou-se a diluição inibitória máxima (DIM) em meio de cultura Agar Mueller Hlnton, das tinturas nas formas pura (1:0) e diluídas de 1:1 até 1:32. Observou-se susceptibilidade variada das bactérias, sendo o S. aureus o microorganismo mais sensível. Dentre as tinturas, o jucá, a aroeira e a própolis apresentaram uma significativa atividade antibacteriana sobre S. mutans, S. sobrinus, S. mitis, S. sanguis e L.casei, sendo que o gengibre e a alfavaca apresentaram os menores espectros de ação frente às linhagens bacterianas avaliadas.