The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats.

OBJECTIVE: This study aimed to evaluate the inflammatory effect and bone formation in sterile surgical failures after implantation of a collagen sponge with mesenchymal stem cells from human dental pulp (hDPSCs) and Aloe vera. MATERIAL AND METHODS: Rattus norvegicus (n=75) were divided into five experimental groups according to treatment: G1) control (blood clot); G2) Hemospon®; G3) Hemospon® in a culture medium enriched with 8% Aloe vera; G4) Hemospon® in a culture medium containing hDPSCs and G5) Hemospon® in a culture medium enriched with 8% Aloe vera and hDPSCs. On days 7, 15 and 30, the animals were euthanized, and the tibia was dissected for histological, immunohistochemistry and immunofluorescence analyses. The results were analyzed using nonparametric Kruskal-Wallis test and Dunn's post-test. RESULTS: On days 7 and 15, the groups with Aloe vera had less average acute inflammatory infiltrate compared to the control group and the group with Hemospon® (p<0.05). No statistically significant difference was found between the groups regarding bone formation at the three experimental points in time. Osteopontin expression corroborated the intensity of bone formation. Fluorescence microscopy revealed positive labeling with Q-Tracker® in hDPSCs before transplantation and tissue repair. CONCLUSION: The results suggest that the combination of Hemospon®, Aloe vera and hDPSCs is a form of clinical treatment for the repair of non-critical bone defects that reduces the inflammatory cascade's effects.

The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats

Abstract Objective This study aimed to evaluate the inflammatory effect and bone formation in sterile surgical failures after implantation of a collagen sponge with mesenchymal stem cells from human dental pulp (hDPSCs) and Aloe vera. Material and Methods Rattus norvegicus (n=75) were divided into five experimental groups according to treatment: G1) control (blood clot); G2) Hemospon®; G3) Hemospon® in a culture medium enriched with 8% Aloe vera; G4) Hemospon® in a culture medium containing hDPSCs and G5) Hemospon® in a culture medium enriched with 8% Aloe vera and hDPSCs. On days 7, 15 and 30, the animals were euthanized, and the tibia was dissected for histological, immunohistochemistry and immunofluorescence analyses. The results were analyzed using nonparametric Kruskal-Wallis test and Dunn's post-test. Results On days 7 and 15, the groups with Aloe vera had less average acute inflammatory infiltrate compared to the control group and the group with Hemospon® (p<0.05). No statistically significant difference was found between the groups regarding bone formation at the three experimental points in time. Osteopontin expression corroborated the intensity of bone formation. Fluorescence microscopy revealed positive labeling with Q-Tracker® in hDPSCs before transplantation and tissue repair. Conclusion The results suggest that the combination of Hemospon®, Aloe vera and hDPSCs is a form of clinical treatment for the repair of non-critical bone defects that reduces the inflammatory cascade's effects.

Aloe vera as vehicle to mineral trioxide aggregate: study in bone repair / Aloe vera como veículo ao mineral trióxido agregado: estudo em reparo ósseo

Aim: Mineral trioxide aggregate (MTA) was associated to Aloe vera to verify the coadjutant action of that medicinal plant in the bone neoformation process in tibia of rats. Material and Method: 36 male rats (Rattus norvegicus) were used, divided into two groups of 18 rats each. Two circumferential bone defects with approximately 5 mm in diameter were made on the right tibia of each animal: the upper defect was filled with blood coagulates in both groups to serve as experimental control and the lower defect was filled with MTA and Aloe vera in experimental (group E1) and MTA and distilled water in experimental (group E2). Seven, 15 and 30 days after surgery, six animals from each group were euthanized and the right tibia of each animal was removed for histological analysis. Result: Histologically, experimental group E1 presented better results for the two variables, inflammation [at seven days (p=0.045)] and bone formation [at seven days (p=0.018) and 30 days (p=0.034)], compared to the E2 group. Conclusion: The association of MTA and Aloe vera showed potential to reduce the effects of the inflammatory cascade and promote bone neoformation making it to a promising proposal for future use in endodontic therapy. .

Objetivo: Mineral trióxido agregado (MTA) foi associado a Aloe vera para se verificar a ação coadjuvante desta planta medicinal no processo de neoformação óssea em tíbia de ratos. Material e Método: 36 ratos machos (Rattus norvegicus) foram utilizados, divididos em dois grupos com 18 animais em cada. Dois defeitos ósseos circunferenciais com aproximadamente 5 mm de diâmetro foram feitos na tíbia direita de cada animal: o defeito superior foi preenchido com coágulo sanguíneo em ambos os grupos para servir como controle e o defeito inferior foi preenchido com MTA e Aloe vera (grupo E1) e MTA e água destilada (grupo E2). Sete, 15 e 30 dias após a cirurgia, seis animais de cada grupo foram submetidos à eutanásia e a tíbia direita de cada animal foi retirada para análise histológica. Resultado: Histologicamente, o grupo experimental E1 apresentou melhores resultados para as duas variáveis, inflamação [em sete dias (p = 0,045)] e formação óssea [em sete dias (p = 0,018) e 30 dias (p = 0,034)], em comparação com os resultados do grupo E2. Conclusão: A associação entre o MTA e Aloe vera demonstrou potencial para reduzir os efeitos da cascata inflamatória e promover a neoformação óssea, tornando-a uma proposta promissora para uso futuro no tratamento endodôntico. .