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1.
Int J Clin Pharmacol Ther ; 47(3): 165-8, 2009 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-19281725

RESUMEN

OBJECTIVE: To report a case of an uncommon and up to date unpublished peracute and overwhelming muscle pain following administration of ciprofloxacin. CASE SUMMARY: A 58-year-old male developed fulminating musculoskeletal pain associated with third-time exposure to ciprofloxacin administered for treatment of chronic otitis media. Symptoms resolved slowly after intense combined analgetic therapy and cessation of ciprofloxacin therapy. 24 h after ciprofloxacin discontinuation the symptoms had completely disappeared and no more analgetic treatment was needed. Laboratory values, especially muscle enzymes, did not reveal any pathological pattern. The patient's past medical history highlighted reproducible side effects with both systemic and local administration of ciprofloxacin including milder symptoms of the musculoskeletal system. DISCUSSION: Common side effects of fluoroquinolones include gastrointestinal, central nervous and allergic reactions, but also more uncommon reactions such as tendonitis and rhabdomyolysis. In our case, there had been no signs of rhabdomyolysis. Besides an elevated IgE level no clinical signs of a true anaphylactic reaction associated with release of mast cell mediators had been observed. A pharmacokinetic interaction between ciprofloxacin and the patient's comedication carbamazepine is unlikely to be the responsible mechanism, since fluoroquinolones inhibit cytochrome P450 isoenzyme CYP1A2 but not CYP3A4 which metabolizes carbamazepine. CONCLUSION: To our knowledge, this is the first report describing fulminating musculoskeletal pain following administration of ciprofloxacin without any signs of rhabdomyolysis. Physicians should notice that there is a variety of adverse reactions of this usually well-tolerated agent and they should be aware of unusual complaints of their patients who receive fluoroquinolone treatment.


Asunto(s)
Antibacterianos/efectos adversos , Ciprofloxacina/efectos adversos , Enfermedades Musculares/inducido químicamente , Dolor/inducido químicamente , Enfermedad Aguda , Antibacterianos/uso terapéutico , Enfermedad Crónica , Ciprofloxacina/uso terapéutico , Urgencias Médicas , Humanos , Inyecciones Intravenosas , Masculino , Persona de Mediana Edad , Enfermedades Musculares/tratamiento farmacológico , Otitis Media/tratamiento farmacológico , Dolor/tratamiento farmacológico
2.
Colloids Surf B Biointerfaces ; 60(1): 7-11, 2007 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-17681765

RESUMEN

The effect of plant growth promoting rhizobacteria (PGPR) like Pseudomonas fluorescens on growth parameters and the production of ajmalicine were investigated in Catharanthus roseus under drought stress. The plants under pot culture were subjected to 10, 15 and 20 days interval drought (DID) stress and drought stress with Pseudomonas fluorescens at 1mgl(-1) and 1mgl(-1)Pseudomonas fluorescens alone from 30 days after planting (DAP) and regular irrigation was kept as control. The plants were uprooted on 41 DAS (10 DID), 46 DAS (15 DID) and 51 DAS (20 DID). Drought stress decreased the growth parameters and increased the ajmalicine content. But the treatment with Pseudomonas fluorescens enhanced the growth parameters under drought stress and partially ameliorated the drought induced growth inhibition by increasing the fresh and dry weights significantly. The ajmalicine content was again increased due to Pseudomonas fluorescens treatment to the drought stressed plants. From the results of this investigation, it can be concluded that, the seedling treatments of native PGPRs can be used as a good tool in the enhancement of biomass yield and alkaloid contents in medicinal plants, as it provides an eco-friendly approach and can be used as an agent in water deficit stress amelioration.


Asunto(s)
Biomasa , Catharanthus/metabolismo , Catharanthus/microbiología , Deshidratación/metabolismo , Pseudomonas fluorescens/fisiología , Alcaloides de Triptamina Secologanina/metabolismo , Agua/metabolismo , Catharanthus/anatomía & histología , Catharanthus/crecimiento & desarrollo
3.
Colloids Surf B Biointerfaces ; 59(2): 150-7, 2007 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-17560094

RESUMEN

Catharanthus roseus (L.) G. Don plants were grown in different water regimes in order to study the drought induced osmotic stress and proline (PRO) metabolism, antioxidative enzyme activities and indole alkaloid accumulation. The plants under pot culture were subjected to 10, 15 and 20 days interval drought (DID) stress from 30 days after sowing (DAS) and regular irrigation was kept as control. The plants were uprooted on 41DAS (10DID), 46DAS (15DID) and 51DAS (20DID). The drought stressed plants showed increased aminoacid (AA), glycine betaine (GB) and PRO contents and decreased proline oxidase (PROX) and increased gamma-glutamyl kinase (gamma-GK) activities when compared to control. The antioxidative enzymes like peroxidase (POX) and polyphenol oxidase (PPO) increased to a significant level in drought stressed plants when compared to control. The drought stressed C. roseus plants showed an increase in total indole alkaloid content in shoots and roots when compared to well-watered control plants. Our results suggest that the cultivation of medicinal plants like C. roseus in water deficit areas would increase its PRO metabolism, osmoregulation, defense system and the level of active principles.


Asunto(s)
Antioxidantes/metabolismo , Catharanthus/enzimología , Alcaloides Indólicos/metabolismo , Equilibrio Hidroelectrolítico/fisiología , Catharanthus/metabolismo , Deshidratación/metabolismo , Ósmosis/fisiología , Agua/metabolismo
4.
Colloids Surf B Biointerfaces ; 59(1): 59-66, 2007 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-17531451

RESUMEN

We have examined the changes in carbohydrate metabolism in food yam (Dioscorea esculenta (Lour.) Burk.) tubers and in an economically important spice cum medicinal plant turmeric (Curcuma longa L.) rhizomes under storage. Both specimens showed varied levels of dormancy and sprouting appeared at the end of dormant period. Harvested, fully matured tubers of yam and rhizomes of turmeric were stored in wooden boxes under the conditions of 28+/-2 degrees C temperature and 65-75% relative humidity (RH) in dark. The starch, sugars, enzymes of starch degradation, respiration, glycolysis, tricarboxylic acid (TCA) cycle and oxidative pentose phosphate pathway (PPP) were studied during 1-70 days after harvest (DAH). This investigation revealed that, the starch degradation and the enzymes involved, viz. alpha-amylases and starch phosphorylase showed a lower level of activity during early period of dormancy, while sugar content and enzymes of carbohydrate metabolism increased rapidly during sprouting. The isoenzymic profiles of alpha-amylases showed marked variations in these two phases. The key enzymes of glycolysis, TCA cycle and PPP, viz. aldolase, succinic dehydrogenase, malic dehydrogenase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were increased even before the visible appearance of sprouting and their activities were at their maximum during sprouting. Based of the observations the dormancy period may be distinctly divided into peak period of rest and presprouting period.


Asunto(s)
Metabolismo de los Hidratos de Carbono , Curcuma/metabolismo , Dioscorea/metabolismo , Ciclo del Ácido Cítrico , Curcuma/crecimiento & desarrollo , Dioscorea/crecimiento & desarrollo , Manipulación de Alimentos , Fructosa-Bifosfato Aldolasa/metabolismo , Glucosafosfato Deshidrogenasa/metabolismo , Glucólisis , Isoenzimas/metabolismo , Malato Deshidrogenasa/metabolismo , Vía de Pentosa Fosfato , Fosfogluconato Deshidrogenasa/metabolismo , Tubérculos de la Planta/crecimiento & desarrollo , Tubérculos de la Planta/metabolismo , Rizoma/crecimiento & desarrollo , Rizoma/metabolismo , Almidón/metabolismo , Almidón Fosforilasa/metabolismo , Succinato Deshidrogenasa/metabolismo , alfa-Amilasas/metabolismo
5.
J Biol Chem ; 274(48): 34361-8, 1999 Nov 26.
Artículo en Inglés | MEDLINE | ID: mdl-10567413

RESUMEN

We previously showed that soluble, pepsin-solubilized collagen VI increases de novo DNA synthesis in serum-starved HT1080 and 3T3 fibroblasts up to 100-fold compared with soluble collagen I, reaching 80% of the stimulation caused by 10% fetal calf serum. Here we show that collagen VI also inhibits apoptotic cell death in serum-starved cells as evidenced by morphological criteria, DNA laddering, complementary apoptosis assays (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, enzyme-linked immunosorbent assay, and fluorescence-activated cell sorting), and quantification of apoptosis-regulating proteins. In the presence of starving medium alone or collagen I, the proapoptotic Bax was up-regulated 2-2.5-fold, compared with soluble collagen VI and fetal calf serum, whereas levels of the antiapoptotic Bcl-2 protein remained unaffected. In accordance with its potent stimulation of DNA synthesis, soluble collagen VI carries serum-starved HT1080 and Balb 3T3 fibroblasts through G(2) as shown by fluorescence-activated cell sorting analysis, whereas cells exposed to medium and collagen I where arrested at G(1)-S. This was accompanied by a 2-3-fold increase in cyclin A, B, and D1 protein expression. Collagen VI-induced inhibition of apoptotic cell death may be operative during embryogenesis, wound healing, and fibrosis when elevated tissue and blood levels of collagen VI are observed, thus initiating a feedback loop of mesenchymal cell activation and proliferation.


Asunto(s)
Apoptosis/efectos de los fármacos , Colágeno/farmacología , Fibroblastos/efectos de los fármacos , Proteínas Proto-Oncogénicas c-bcl-2 , Proteínas Proto-Oncogénicas/efectos de los fármacos , Fase S , Células 3T3 , Animales , Adhesión Celular/efectos de los fármacos , Ciclo Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Medio de Cultivo Libre de Suero/farmacología , Ciclina A/efectos de los fármacos , Ciclina A/metabolismo , Ciclina B/efectos de los fármacos , Ciclina B/metabolismo , Ciclina D1/efectos de los fármacos , Ciclina D1/metabolismo , Fragmentación del ADN/efectos de los fármacos , Regulación hacia Abajo , Fibroblastos/citología , Fibroblastos/fisiología , Humanos , Etiquetado Corte-Fin in Situ , Integrina beta1/fisiología , Ratones , Proteínas Proto-Oncogénicas/metabolismo , Solubilidad , Células Tumorales Cultivadas , Regulación hacia Arriba , Proteína X Asociada a bcl-2
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