Development of lamellar gel phase emulsion containing marigold oil (Calendula officinalis) as a potential modern wound dressing.

Appropriate therapeutics for wound treatments can be achieved by studying the pathophysiology of tissue repair. Here we develop formulations of lamellar gel phase (LGP) emulsions containing marigold (Calendula officinalis) oil, evaluating their stability and activity on experimental wound healing in rats. LGP emulsions were developed and evaluated based on a phase ternary diagram to select the best LGP emulsion, having a good amount of anisotropic structure and stability. The selected LGP formulation was analyzed according to the intrinsic and accelerated physical stability at different temperatures. In addition, in vitro and in vivo studies were carried out on wound healing rats as a model. The LGP emulsion (15.0% marigold oil; 10.0% of blend surfactants and 75.0% of purified water [w/w/w]) demonstrated good stability and high viscosity, suggesting longer contact of the formulation with the wound. No cytotoxic activity (50-1000 µg/mL) was observed in marigold oil. In the wound healing rat model, the LGP (15 mg/mL) showed an increase in the leukocyte recruitment to the wound at least on days 2 and 7, but reduced leukocyte recruitment after 14 and 21 days, as compared to the control. Additionally, collagen production was reduced in the LGP emulsion on days 2 and 7 and further accelerated the process of re-epithelialization of the wound itself. The methodology utilized in the present study has produced a potentially useful formulation for a stable LGP emulsion-containing marigold, which was able to improve the wound healing process.

Inhibiting effect of Dorstenia asaroides extracts on cariogenic properties of Streptococcus mutans.

The aim of this study was to examine the effects of Dorstenia asaroides extracts on cariogenic properties of the most cariogenic bacteria, Streptococcus mutans. Hexane (HFr), ethyl-acetate (EFr) and chloroform (CFr) extracts obtained from D. asaroides rhizomes were submitted to chemical analyses, Minimal Inhibitory Concentrations (MIC), glycolysis assay and S. mutans 12-h-old initial biofilms. Chemical characterization showed that all the extracts present furanocoumarins. The MIC values were 80 (HFr and CFr) and 50 µg/mL (EFr). Acid production by S. mutans cells was significantly disrupted by HFr (12.5 mg/mL), EFr (at 2.5; 6.25 and 12.5 mg/mL) and CFr (at 2.5, 6.25 and 12.5 mg/mL) (p < 0.01). Topical applications of HFr, EFr and CFr significantly reduced the colony forming units of S. mutans biofilms compared with those treated with control group in order to 20, 30 and 25% respectively (p < 0.01). The results of the present study suggest that rhizomes of D. asaroides had inhibitory effects on cariogenic properties of S. mutans.

Assessment of the potential genotoxic risk of medicinal Tamarindus indica fruit pulp extract using in vivo assays.

Tamarindus indica has been used in folk medicine as an antidiabetic, a digestive aid, and a carminative, among other uses. Currently, there is no information in the toxicology literature concerning the safety of T. indica extract. We evaluated the clastogenic and/or genotoxic potential of fruit pulp extract of this plant in vivo in peripheral blood and liver cells of Wistar rats, using the comet assay, and in bone marrow cells of Swiss mice, using the micronucleus test. The extract was administered by gavage at doses of 1000, 1500 and 2000 mg/kg body weight. Peripheral blood and liver cells from Wistar rats were collected 24 h after treatment, for the comet assay. The micronucleus test was carried out in bone marrow cells from Swiss mice collected 24 h after treatment. The extract made with T. indica was devoid of clastogenic and genotoxic activities in the cells of the rodents, when administered orally at these three acute doses.

Neuromuscular diagnosis in orthodontics: effects of TENS on the sagittal maxillo-mandibular relationship.

AIM: This study was conducted in order to assess the changes in the occlusal position of the mandible after ULF (Ultra Low Frequency)-TENS relaxing procedure in subjects in pubertal growth phase with diagnosed Angle Class II division 1 and mandibular dentoalveolar retrusion. MATERIALS AND METHODS: This study was performed on 19 patients (13 females, 6 males) with an Angle Class II division 1, aged between 10 and 15 years old (mean age 12.26, SD 1.32), characterised by mandible dentoalveolar retrusion and optimal vertical facial dimension, diagnosed by clinical and cephalometric evaluation. Diagnostic neuromuscular registrations were made for all subjects. The casts were mounted on articulator in habitual intercuspal position with a tooth-guided wax bite registration. Reference points were chosen at molar level. Subsequently the same casts were mounted in myocentric position and compared to the habitual intercuspal position, assessing the sagittal shift after TENS procedure. STATISTICS: Mean and standard deviation were calculated on the amount of shifting at the left molar reference point after TENS procedure. Analysis of variance (ANOVA), using STATA statistics package, was carried out in order to evaluate the influence of sex and age on the amount of molar shift. RESULTS: Nine subjects showed, in the sagittal plane, a forward mandibular shift in neuromuscular myocentric position compared to habitual intercuspal position. Six subjects showed no differences between habitual and myocentric position in the sagittal plane. Four individuals showed a backward mandible shift after TENS indicating worsening of the II molar class in the sagittal plane. CONCLUSION: This study suggests that TENS recorded occlusion in subjects with Class II division 1 with mandible dentoalveolar retrusion allows to visualise an unusual trend of growth. The advancements of the mandible were not taken into account. These results could offer new diagnosis and prognosis methods for Class II malocclusions.

Osteopathic manipulative treatment (OMT) effects on mandibular kinetics: kinesiographic study.

AIM: The aim of this study was to evaluate the effects of Osteopathic Manipulative Treatment (OMT) on mandibular kinematics in TMD patients. METHODS: The study was conduced on 28 children with non-specific TMD symptoms, limited mouth opening, history of trauma (delivery trauma, accident trauma). Patients were randomly divided into two groups: an OMT group (study group) and a no-intervention group (control group). All subjects underwent a first kinesiographic recording to evaluate the amplitude and velocity of maximal opening-closing movements. Study group patients underwent a second kinesiographic recording 2 months after OMT. Control group patients were submitted to a control kinesiographic recording six months after the first one. Kinesiographic tracings were acquired using the K7I system. RESULTS/STATISTICS: The kinesiographic data of the study group showed a moderate statistically significant difference (p<.07) of maximal mouth opening (MO) parameter and a high statistically significant difference (p<.03) of maximal mouth opening velocity (MOV) parameter. No statistically significative difference (null hypothesis confirmed) of kinesiographic parameters in the control group was observed. CONCLUSION: The results of this study suggest that OMT can induce changes in the stomatognathic dynamics, offering a valid support in the clinical approach to TMD. Multifactorial genesis of chronic disorders is also confirmed.