[Analysis of broad-sense heritability and genetic correlation of production and content of glycyrrhizin of annual Glycyrrhiza uralensis].

OBJECTIVE: To estimate the broad-sense heritability of the production of Glycyrrhiza uralensis and the content of glycyrrhizin as well as the genetic relationship of various growth indexes and biomass indexes, and provide the scientific basis for establishment of high quality licorice cultivate technology system. METHOD: The randomized method was used to assign the provenance trial, the content of glycyrrhizin was determined by HPLC, and the method of classic genetics was applied to estimate the broad-sense heritability and genetic correlation coefficient. RESULT AND CONCLUSION: The content of glycyrrhizin is influenced by the growth environment and gene, but the growth environment is the dominant factor. The estimated result of single sites about broad-sense heritability (h2) showed that the production of G. uralensis (W(u)) and the content of glycyrrhizin was controlled by gene which the broad-sense heritability was 0.663 2, 0.751 1 respectively, they had some potential on genetic modification. The results of genetic analysis correlation showed that the plant height and the stem diameter was positive (P < 0.01) correlated significantly with the production (W(u)) either on phenotype or on genetic, it suggests that the plant height and the stem diameter could be the index above ground to assessment the production of the G. uralensis. The content of glycyrrhizin had a positive correlation with the number of lateral root (P < 0.05), but it had a negative correlation with the plant height, stem diameter, diameter of root top (D(r)), the total biomass (W(t)) and the biomass underground (W(u)) on inheritance. It is suggested that it was difficult to achieve both high content and high yield simultaneously in the genetic improvement, so we should have a deeply thought about the specific improvement target when making the reformed scheme.

[The underground part growth distribution pattern of Glycyrrhiza uralensis and its effects on glycyrrhizinic acid content].

OBJECTIVE: To ascertain the relationship between glycyrrhizinic acid content and the underground part growth character of Glycyrrhiza uralensis and provide the theoretical evidence for wild resources protection and artificial cultivation method of G. uralensis. METHOD: Through the analytical investigation on the underground part of G. uralensis and analysis of glycyrrhizinic acid content in different organs, parts, ages, and diameter medicinal materials, the systematic study on the relationship between glycyrrhizinic acid content and the underground part growth character of glycyrrhiza uralensis was carried out. RESULT: The underground part of a G. uralensis seedling consisted of seed root, random root, horizontal underground stem, vertical underground stem and assimilating root. The glycyrrhizinic acid content in horizontal underground stem with the age below two years old or in random root with the diameter below 0.5 cm was low. The difference of glycyrrhizinic acid content among horizontal underground stem, random root and vertical underground stem was obvious, but the difference between horizontal underground stem and random root was not obvious. CONCLUSION: The horizontal underground stem was of G. uralnesis acts as a link that can connect random root, vertical underground and stem assimilating root, so that the whole underground part constructs one huge underground net system. The glycyrrhizinic acid accumulation is a effected by organ type, growth age, root diameter and grow position, and the distribution pattern of random root and vertical underground stem has influence on glycyrrhizinic acid distribution in horizontal underground stem.