RESUMEN
This paper aimed to investigate the botanical origins of Isatidis Radix and Isatidis Folium, and clarify the confusion of its classification. The second internal transcribed spacer (ITS2) of ribosomal DNA, the chloroplast matK gene of 22 samples from some major production areas were amplified and sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. Phylogenetic study was performed using MEGA 4.0 software in accordance with the Kimura 2-Parameter (K2P) model, and the phylogenetic tree was constructed using the neighbor-joining methods. The results showed that the length of ITS2 sequence of the botanical origins of Isatidis Radix and Isatidis Folium was 191 bp. The sequence showed that some samples had several SNP sites, and some samples had heterozygosis sites. In the NJ tree, based on ITS2 sequence, the studied samples were separated into two groups, and one of them was gathered with Isatis tinctoria L. The studied samples also were divided into two groups obviously based on the chloroplast matK gene. In conclusion, our results support that the botanical origins of Isatidis Radix and Isatidis Folium are Isatis indigotica Fortune, and Isatis indigotica and Isatis tinctoria are two distinct species. This study doesn't support the opinion about the combination of these two species in Flora of China.
Asunto(s)
Código de Barras del ADN Taxonómico/métodos , ADN Espaciador Ribosómico/genética , Isatis/clasificación , Plantas Medicinales/clasificación , Cloroplastos/genética , ADN de Plantas/genética , Genes de Plantas/genética , Isatis/genética , Filogenia , Hojas de la Planta/genética , Plantas Medicinales/genética , Especificidad de la EspecieRESUMEN
OBJECTIVE: Classification and diagnostic study on Lepidiumn (Brassicaceae) from China. METHODS: Leaf epidermal mi-cromophology of 10 species of Lepidium from China were observed by using LM (light microscope) and SEM (scaning electron microscope). RESULTS: The stomatal apparatuses present both on the adaxial epidermis and the abaxial epidermis. The type of stomatal apparatuses is mainly anisocytic, rarely anomocytic and only occasionally paracytic. The leaf epidermal cells of Lepidium are usually irregular or polygonal in shape. The patterns of anticlinal walls are straight, arched, sinuolate or sinuous. The leaf features of Lepidium have better consistency. The morphology of the upper epidermis and the lower epidermis is similar, and with simple trichomes or glabrous on the leaf surface. Under SEM observation, the inner margin of the outer stomatal rim is nearly smooth or sinuolate, and the cuticular membrane of the leaf epidermis is striate. The shapes of leaf epidermal cells, the patterns of anticlinal walls, the types of stomatal ap-paratuses, the stomatal index, the stomatal size and the characters of the cuticular membrane of the leaf epidermis have important reference for differentiating species of Lepidium. CONCLUSION: The leaf epidermal features can serve as a criterion of distinguishing species in the genus Lepidium (Brassicaceae) from China, and also provide new evidence for rational use and exploitation of medical resources in Lepidium.