Fatty Acid Profile and Mineral Composition of Red-Toothed Triggerfish (Odonus niger) Landed in Karnataka, Southeastern Arabian Sea.

The seasonal and sex-wise variations in fatty acid and mineral composition in the tissue of Odonus niger collected from the Karnataka coast, southeastern Arabian Sea, were evaluated for the first time. The fatty acid profile was assessed using gas chromatography, nutritional indices were employed to evaluate lipid quality, and standard methods were utilized to estimate mineral composition and heavy metals. Palmitic acid (20.2-45.9%), oleic acid (10.0-19.2%), and docosahexaenoic acid (10.9-36.7%) were found in the highest proportions. The amount of ω3 fatty acids was significantly higher than ω6 fatty acids indicating the healthiest food fish and a promising source for nutrient supplementation. The P/S (PUFA/SFA) and ω3/ω6 ratios of the species were greater than those recommended by the UK Dept. of Health. Index of atherogenicity (IA) and thrombogenicity (IT) was low, and hypocholesterolemic to the hypercholesterolemic ratio (HH), unsaturation index (UI), health-promoting index (HPI), fish lipid quality (FLQ), and polyene index (PI) were high. The relationship between the quantities of macronutrients and trace elements was calculated as K > P > Na > Mg > Ca and B > Fe > Zn > Ga > Al respectively. Heavy metals like Be, Bi, Co, and Hg were found below the detection level. Benefit-risk ratio value indicates that the species is safe for consumption.

Dietary Administration of Engineered Nano-selenium and Vitamin C Ameliorates Immune Response, Nutritional Physiology, Oxidative Stress, and Resistance Against Aeromonas hydrophila in Nile Tilapia (Oreochromis niloticus).

Functional trace elements and vitamins can boost immunity and anti-oxidative response in aquatic animals with effects on nutritional physiology. Nano-selenium (nano-Se) and vitamin C (VC) have been used as immunomodulators and antioxidants in fish feed. The present work was performed to determine the protective effects of diets supplemented with different combinations of nano-Se and VC on Nile tilapia (Oreochromis niloticus). Triplicate groups of 20 fish/tank (13.87 ± 0.10 g) were reared and fed with basal diet (control-T1) (without supplementation of nano-Se and VC) and three experimental diets as T2, T3, and T4 (100, 200, and 300 mg/kg VC respectively) with a pre-determined dose of nano-Se (1.0 mg/kg) for 90 days. Different immune indices, haemato-biochemical, and antioxidant activities were measured at the end of the first, second, and third months of feeding. The findings depicted that significantly (p < 0.05) higher growth was observed in T4. Red blood cells, white blood cells, and haemoglobin were found significantly (p < 0.05) higher in T4 for the third month. Serum biochemical-immunological indices (alkaline phosphatase, glucose, cholesterol, lysozyme, myeloperoxidase, total protein, albumin and globulin) followed the same trend. Furthermore, antioxidant assays such as catalase, superoxide dismutase, glutathione peroxidase, glutathione S-transferase, and malondialdehyde were significantly (p < 0.05) improved in T4 for the third month. Significantly (p < 0.05) least cumulative mortality against Aeromonas hydrophila was obtained in the fish-fed diets incorporated with nano-Se and VC. Therefore, dietary supplementation with nano-Se and VC is noteworthy for improving growth, serum biochemical status, immune response, antioxidant status, and disease resistance.