RESUMEN
OBJECTIVE: Critical lower-limb ischemia (CLLI) is characterized by high morbidity and mortality. The aim of this study was to explore the effectiveness of the combination of cell therapy with apelin-13 and hyperbaric oxygen in CLLI animal model. MATERIALS AND METHODS: The experimental ischemic rats were divided into five groups, including negative control, bone marrow derived mononuclear cells (BM-MNCs), apelin-13, hyperbaric oxygen treatment (HBOT) and apelin-13 with HBOT group. Each group was composed of 10 rats. Endothelial progenitor cells (EPCs) derived from bone marrow were transplanted into the ischemia rat model. After 3 weeks of transplantation, the formation of new vessels was evaluated by examining cluster of differentiation (CD)31, CD34 and vascular endothelial growth factor receptor 2 (VEGFR-2) expressions as well as a direct vision of vessels by hematoxylin and eosin (HE) staining and immunohistochemistry. RESULTS: Compared with the negative control group, both angiogenic factors expressions and the number of new vessels increased notably by the transplantation of BM-MNCs in the ischemic models. Apelin-13 or HBOT alone improved the efficacy within limit while the combination of the three elements remarkably promoted the neovascularization in ischemic limbs. CONCLUSIONS: BM-MNC induced angiogenesis in the ischemic limbs and was considered an effective resource for cell therapy. The preliminary data of this study showed that the combination of cell therapy with apelin-13 and HBOT improved the efficacy of angiogenesis.
Asunto(s)
Células Progenitoras Endoteliales/trasplante , Miembro Posterior/irrigación sanguínea , Péptidos y Proteínas de Señalización Intercelular/farmacología , Isquemia/terapia , Animales , Antígenos CD34/metabolismo , Terapia Combinada , Modelos Animales de Enfermedad , Células Progenitoras Endoteliales/metabolismo , Regulación de la Expresión Génica , Oxigenoterapia Hiperbárica , Isquemia/metabolismo , Masculino , Neovascularización Fisiológica , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/metabolismo , Ratas , Receptor 2 de Factores de Crecimiento Endotelial Vascular/metabolismoRESUMEN
Four flavanoids were isolated from the seed of Hovenia dulcis Thunb. On the basis of physicochemical properties and spectroscopic analysis, their structures were identified as dihydrokaempferol (I), quercetin (II), (+)-3,3',5',5,7-pentahydroflavanone (III) and (+)-dihydromyricetin (IV). Among them, III was a new compound; I and IV were first isolated from genus Hovenis.