RESUMEN
We studied the effect of dietary supplementation with grape seed proanthocyanidins extract (GSPE) 3 mg added in 100 g high-calcium diet with a calcium content of 1697 mg 100 g(-1) on mandibular condyle bone debility, which was induced by a low-calcium diet. Forty Wistar male rats, 5 week old, were randomly divided into control (Co), low-calcium diet (LC), low-calcium/high-calcium diet (LCH), and low-calcium/high-calcium with supplementary GSPE diet (LCHG) groups for 6 wk. Bone formation of the mandibular condyle was measured using peripheral quantitative computed tomography (pQCT). Significant differences were not seen among the four groups for body weight, measured weekly. The LCHG group scored significantly higher in cortical bone density, total bone cross-sectional area, cortical bone cross-sectional area, cortical bone mineral content, total bone density, total bone mineral content, and in the stress-strain index to the reference axis x when compared with the LCH group. We concluded that a high-calcium diet combined with GSPE supplementation is more effective in reversing mandibular condyle bone debility in rats than is a low-calcium diet, standard diet, or high-calcium diet alone.
Asunto(s)
Cóndilo Mandibular/efectos de los fármacos , Enfermedades Mandibulares/tratamiento farmacológico , Osteogénesis/efectos de los fármacos , Fitoterapia , Extractos Vegetales/uso terapéutico , Proantocianidinas/uso terapéutico , Semillas , Vitis , Anatomía Transversal , Animales , Peso Corporal/efectos de los fármacos , Densidad Ósea/efectos de los fármacos , Calcio/deficiencia , Calcio de la Dieta/uso terapéutico , Elasticidad , Masculino , Cóndilo Mandibular/diagnóstico por imagen , Enfermedades Mandibulares/diagnóstico por imagen , Minerales/análisis , Distribución Aleatoria , Ratas , Ratas Wistar , Estrés Mecánico , Tomografía Computarizada por Rayos XRESUMEN
Grape seed proanthocyanidins extract (GSPE), whose principal ingredient is proanthocyanidins, shows many activities such as cholesterol lowering effects, antioxidant effects, anti-tumor effects, cardioprotective effects, and protection against ultraviolet rays. However, reports of the effects of GSPE on bone are rare. We performed a mechanical analysis of the effect of GSPE on the interior structure of rat mandibular bone in the growth period, using three-dimensional peripheral quantitative computed tomography (pQCT). A low-calcium/high-calcium diet with supplementary GSPE was compared to a low-calcium/high-calcium diet in rats with debilitated mandibular bones. The group who received added GSPE showed a significant increase in cortical bone density, cross-sectional area, and trabecular bone mineral content (p<0.05). A significant increase was also seen in the results of a non-invasive stress strain index (SSI) (p<0.01) in the added GSPE. Our findings suggest that GSPE can increase bone quality and bone strength of rat mandibles in the growth period.