RESUMEN
PURPOSE: To investigate the effects of baicalin on inflammatory reaction, oxidative stress and protein kinase D1 (PKD1) and nuclear factor-kappa B (NF-κB) protein expressions in severe acute pancreatitis (SAP) rats. METHODS: Sixty rats were divided into sham operation, model, and low-, medium- and high-dose baicalin group. SAP model was established in later 4 groups. The later 3 groups were injected with 0.1, 0.2 and 0.4 ml/100 g 5% baicalin injection, respectively. At 12 h, the serum SAP related indexes and inflammatory factors, peripheral blood CD3 and γδT cell percentages, wet/dry ratio and pancreas ascites volume, oxidative stress indexes and PKD1 and NF-κB protein expressions in pancreatic tissue were determined. RESULTS: Compared with model group, in high-dose baicalin group the wet/dry ratio and ascites volume, serum amylase level, phospholipase A2 activity, TNF-α, IL-1 and IL-6 levels, and pancreatic malondialdehyde level and PKD1 and NF-κB protein expression were significantly decreased (P < 0.05), and peripheral blood CD3 and γδT cell percentages and pancreatic superoxide dismutase and glutathione peroxidase levels were significantly increased (P < 0.05). CONCLUSION: Baicalin can resist the inflammatory reaction and oxidative stress, and down-regulate protein kinase D1 and nuclear factor-kappa B protein expressions, thus exerting the protective effects on severe acute pancreatitis in rats.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Flavonoides/farmacología , FN-kappa B/metabolismo , Estrés Oxidativo/efectos de los fármacos , Pancreatitis/tratamiento farmacológico , Proteína Quinasa C/metabolismo , Amilasas/sangre , Amilasas/efectos de los fármacos , Animales , Complejo CD3/sangre , Complejo CD3/efectos de los fármacos , Regulación hacia Abajo/efectos de los fármacos , Glutatión Peroxidasa/efectos de los fármacos , Glutatión Peroxidasa/metabolismo , Interleucina-1/sangre , Interleucina-6/sangre , Malondialdehído/metabolismo , FN-kappa B/efectos de los fármacos , Pancreatitis/metabolismo , Proteína Quinasa C/efectos de los fármacos , Distribución Aleatoria , Ratas Sprague-Dawley , Reproducibilidad de los Resultados , Superóxido Dismutasa/efectos de los fármacos , Superóxido Dismutasa/metabolismo , Resultado del Tratamiento , Factor de Necrosis Tumoral alfa/sangre , Factor de Necrosis Tumoral alfa/efectos de los fármacosRESUMEN
Abstract Purpose: To investigate the effects of baicalin on inflammatory reaction, oxidative stress and protein kinase D1 (PKD1) and nuclear factor-kappa B (NF-κB) protein expressions in severe acute pancreatitis (SAP) rats. Methods: Sixty rats were divided into sham operation, model, and low-, medium- and high-dose baicalin group. SAP model was established in later 4 groups. The later 3 groups were injected with 0.1, 0.2 and 0.4 ml/100 g 5% baicalin injection, respectively. At 12 h, the serum SAP related indexes and inflammatory factors, peripheral blood CD3 and γδT cell percentages, wet/dry ratio and pancreas ascites volume, oxidative stress indexes and PKD1 and NF-κB protein expressions in pancreatic tissue were determined. Results: Compared with model group, in high-dose baicalin group the wet/dry ratio and ascites volume, serum amylase level, phospholipase A2 activity, TNF-α, IL-1 and IL-6 levels, and pancreatic malondialdehyde level and PKD1 and NF-κB protein expression were significantly decreased (P < 0.05), and peripheral blood CD3 and γδT cell percentages and pancreatic superoxide dismutase and glutathione peroxidase levels were significantly increased (P < 0.05). Conclusion: Baicalin can resist the inflammatory reaction and oxidative stress, and down-regulate protein kinase D1 and nuclear factor-kappa B protein expressions, thus exerting the protective effects on severe acute pancreatitis in rats.
Asunto(s)
Animales , Pancreatitis/tratamiento farmacológico , Flavonoides/farmacología , Proteína Quinasa C/metabolismo , Antiinflamatorios no Esteroideos/farmacología , FN-kappa B/metabolismo , Estrés Oxidativo/efectos de los fármacos , Pancreatitis/metabolismo , Superóxido Dismutasa/efectos de los fármacos , Proteína Quinasa C/efectos de los fármacos , Distribución Aleatoria , Regulación hacia Abajo/efectos de los fármacos , Reproducibilidad de los Resultados , FN-kappa B/efectos de los fármacos , Interleucina-6/sangre , Interleucina-1/sangre , Factor de Necrosis Tumoral alfa/sangre , Resultado del Tratamiento , Ratas Sprague-Dawley , Complejo CD3/efectos de los fármacos , Complejo CD3/sangre , Glutatión Peroxidasa/efectos de los fármacos , Glutatión Peroxidasa/metabolismo , Amilasas/efectos de los fármacos , Amilasas/sangre , Malondialdehído/metabolismoRESUMEN
The purpose of this study is to report the first nationwide protocol (Wuhan Protocol) developed by Chinese Children's Cancer Group and the results of multidisciplinary effort in treating hepatoblastoma. In this study, we reported the final analysis, which includes 153 hepatoblastoma patients in 13 hospitals from January 2006 to December 2013. The 6-year overall survival and event-free survival rates were 83.3 ± 3.1% and 71.0 ± 3.7%, respectively, in this cohort. The univariate analysis revealed that female (P = 0.027), under 5 years of age (P = 0.039), complete surgical resection (P = 0.000), no metastases (P = 0.000), and delayed surgery following neoadjuvant chemotherapy (P = 0.000) had better prognosis. In multivariate analysis, male, 5 years of age or above, stage PRETEXT III or IV, and incomplete surgical resection were among the some adverse factors contributing to poor prognosis. The preliminary results from this study showed that patients who underwent treatment following Wuhan Protocol had similar OS and EFS rates compared to those in developed countries. However, the protocol remains to be further optimized in standardizing surgical resection (including liver transplantation), refining risk stratification and risk-based chemotherapy.