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1.
Biomed Res Int ; 2020: 6897497, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33123582

RESUMEN

INTRODUCTION: In our days, several approaches reported the use of natural compounds in medical applications. Among them, pectin and allantoin are nontoxic, biocompatible, and biodegradable; however, its use for possible wound healing therapeutics is still limited. Pectin and allantoin have been applied in pharmaceutical industry and beauty cosmetic and could be also applied as scaffolds for tissue regeneration, wound healing, and so on. The aim of this study was to combine by the first time two natural ingredients to develop a new biomaterial to treat skin injuries in a rat model. METHODS: For the hydrogel development, new synthesis parameters were established for the obtaining of the film such as temperature, mixing velocity and time, and drying temperatures as well. To enrich the film, the allantoin concentrations were set at 90 wt% and 100 wt% of pectin used. By in vivo assay, films were tested in wound healing in female Wistar rats, 190 ± 10 g in weight and 2 months aged. RESULTS: The obtained films comprise 2 well-differentiated layers, one layer rich in allantoin, which will be the regenerative layer, and one rich in pectin, which will work as an antimicrobial and protective layer to the wound. These were characterized by swelling kinetics, Fourier transform of the infrared spectrum of absorption (FTIR) spectroscopy, and contact angle. The morphology and topography were determined by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). In vivo assay showed remarkable reduce in a time period in a wound healing process when the film was used. The results show that the use of PA (Pectin-Allantoin) hydrogel reduces the total healing time by 25% approximately. CONCLUSIONS: Pectin-Allantoin (PA) film has potential use in medical applications as wound healing material promoting healthy tissue renewal.


Asunto(s)
Alantoína/química , Alantoína/farmacología , Pectinas/química , Pectinas/farmacología , Cicatrización de Heridas/efectos de los fármacos , Animales , Materiales Biocompatibles/química , Modelos Animales de Enfermedad , Femenino , Hidrogeles/química , Hidrogeles/farmacología , Microscopía Electrónica de Rastreo/métodos , Ratas , Ratas Wistar , Espectroscopía Infrarroja por Transformada de Fourier/métodos
2.
Biomed Res Int ; 2020: 1425402, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32382527

RESUMEN

INTRODUCTION: Tissue engineering is an elementary necessity for several applications in the biomedical field through the use of several biopolymers derived from plants. Larrea tridentata (LT) is a very used plant for various medicinal applications with interesting properties; however, its use into cellulose hydrogels for possible regenerative therapeutics is still limited. Cellulose films could be applied in medical field as wound healing, scaffold for connective tissue for periodontal applications, and so on. The aim of this study was to evaluate the mechanical properties and in vivo and in vitro biocompatibility of cellulose hydrogels that have been enriched with LT in a rat model. METHODS: By in vivo and in vitro assays, the concentration of LT was varied from 1 to 5 wt%, respectively. Hydrogel films were implanted intramuscularly into female Wistar rats, 250 g in weight and aged 2 months, to analyze their cytocompatibility and biocompatibility. RESULTS: No case showed any evidence of inflammation or toxicity. Regarding cell morphology and adhesion, the prepared LT cellulose films had better cytocompatibility values than when polystyrene (PS) dishes were used as the control. In all cases, the results suggest that the addition of LT to the hydrogel films did not affect their cytocompatibility or biocompatibility properties and increases their clinical application due to the reported uses of LT. CONCLUSIONS: Cellulose hydrogel films enriched with LT have the potential to be used in the biomedical field acting as regenerative scaffolds.


Asunto(s)
Celulosa , Hidrogeles , Larrea/química , Ensayo de Materiales , Membranas Artificiales , Animales , Celulosa/química , Celulosa/farmacología , Evaluación Preclínica de Medicamentos , Femenino , Hidrogeles/síntesis química , Hidrogeles/química , Hidrogeles/farmacología , Ratones , Células 3T3 NIH , Ratas , Ratas Wistar
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