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Genetika ; 46(12): 1626-34, 2010 Dec.
Artículo en Ruso | MEDLINE | ID: mdl-21434415

RESUMEN

Binary vectors for Agrobacterium-mediated transformation were constructed to express the genes for antimicrobial peptides (APs) of the cectropin-melittin type under the control of the cauliflower mosaic virus 35S RNA promoter in plants. It was shown with Escherichia coli and Agrobacterium tumefaciens cells that the cassettes could be cloned in pB1121-based vectors with deletion of the 3-D-glycuronidase gene only in the orientation opposite to that of the original vector. Transgenic potato plants were obtained using the Belarussian varieties Odyssey, Vetraz, and Scarb. Their cells expressed the MsrA1 or CEMA peptides of the cecropin-melittin type. The expression was shown to confer higher resistance to bacterial (Erwinia carotovora) infection and extremely high resistance to fungal (Phytophtora infestans and Alternarla solani) infections.


Asunto(s)
Péptidos Catiónicos Antimicrobianos/biosíntesis , Meliteno/biosíntesis , Solanum tuberosum/metabolismo , Alternaria/fisiología , Péptidos Catiónicos Antimicrobianos/genética , Caulimovirus/genética , Inmunidad Innata , Meliteno/genética , Pectobacterium carotovorum/fisiología , Phytophthora infestans/fisiología , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/metabolismo , Plantas Modificadas Genéticamente/microbiología , Regiones Promotoras Genéticas , ARN Viral/genética , Solanum tuberosum/genética , Solanum tuberosum/microbiología
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