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1.
Carbohydr Polym ; 262: 117943, 2021 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-33838820

RESUMEN

Aspergillus spp. are well-known producers of pectinases commonly used in the industry. Aspergillus aculeatinus is a recently identified species but poorly characterized. This study aimed at giving a comprehensive characterization of the enzymatic potential of the O822 strain to produce Rhamnogalacturonan type I (RGI)-degrading enzymes. Proteomic analysis identified cell wall degrading enzymes (cellulases, hemicellulases, and pectinases) that accounted for 92 % of total secreted proteins. Twelve out of fifty proteins were identified as RGI-degrading enzymes. NMR and enzymatic assays revealed high levels of arabinofuranosidase, arabinanase, galactanase, rhamnogalacturonan hydrolases and rhamnogalacturonan acetylesterase activities in aqueous extracts. Viscosity assays carried out with RGI-rich camelina mucilage confirmed the efficiency of enzymes secreted by O822 to hydrolyze RGI, by decreasing viscosity by 70 %. Apple juice trials carried out at laboratory and pilot scale showed an increase in filtration flow rate and yield, paving the way for an industrial use of enzymes derived from A. aculeatinus.


Asunto(s)
Aspergillus/enzimología , Filtración/métodos , Jugos de Frutas y Vegetales , Proteínas Fúngicas/metabolismo , Ramnogalacturonanos/metabolismo , Metabolismo de los Hidratos de Carbono , Celulasas/metabolismo , Manipulación de Alimentos/métodos , Glicósido Hidrolasas/metabolismo , Hidrolasas/metabolismo , Malus , Pectinas/metabolismo , Poligalacturonasa/metabolismo , Proteómica
2.
Carbohydr Polym ; 248: 116752, 2020 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-32919555

RESUMEN

Rhamnogalaturonans I (RGI) pectins, which are a major component of the plant primary cell wall, can be recalcitrant to digestion by commercial enzymatic cocktails, in particular during fruit juice clarification process. To overcome these problems and get better insights into RGI degradation, three RGI degrading enzymes (RHG: Endo-rhamnogalacturonase; ABF: α-Arabinofuranosidases; GAN: Endo-ß-1,4-galactanase) from Aspergillus aculeatinus were expressed in Pichia pastoris, purified and fully biochemically characterized. All three enzymes showed acidic pH optimum, and temperature optima between 40-50 °C. The Km values were 0.5 mg.ml-1, 1.64 mg.ml-1 and 3.72 mg.ml-1 for RHG, ABF, GAN, respectively. NMR analysis confirmed an endo-acting mode of action for RHG and GAN, and exo-acting mode for ABF. The application potential of these enzymes was assessed by measuring changes in viscosity of RGI-rich camelina mucilage, showing that RHG-GAN enzymes induced a decrease in viscosity by altering the structures of the RGI backbone and sidechains.


Asunto(s)
Aspergillus/enzimología , Proteínas Fúngicas/metabolismo , Pectinas/metabolismo , Aspergillus/genética , Aspergillus/metabolismo , Pared Celular/química , Estabilidad de Enzimas , Proteínas Fúngicas/genética , Glicósido Hidrolasas/genética , Glicósido Hidrolasas/metabolismo , Concentración de Iones de Hidrógeno , Cinética , Espectroscopía de Resonancia Magnética , Pichia/genética , Polisacárido Liasas/genética , Polisacárido Liasas/metabolismo , Proteínas Recombinantes/metabolismo , Temperatura
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