RESUMEN
Activity of glutamate decarboxylase, a GABA synthesis enzyme, and intensity of its reception in the adrenal cortex and hypothalamus of guinea pigs and rats with normal and stimulated steroidogenesis was investigated. It has been shown that in the adrenal cortex there is a metabolic system which provides GABA synthesis from glutamate and mechanisms of GABA reception by plasmatic membranes. Mediator synthesis in the adrenal cortex is subjected to seasonal changes, GABA synthesis and reception selectively vary with administration of ACTH, prolactin and maintenance of animals on a diet with an excess of potassium ions.
Asunto(s)
Corteza Suprarrenal/enzimología , Receptores de GABA/metabolismo , Ácido gamma-Aminobutírico/biosíntesis , Corteza Suprarrenal/efectos de los fármacos , Hormona Adrenocorticotrópica/farmacología , Animales , Radioisótopos de Carbono , Membrana Celular/efectos de los fármacos , Membrana Celular/enzimología , Cosintropina/farmacología , Glutamato Descarboxilasa/efectos de los fármacos , Glutamato Descarboxilasa/metabolismo , Cobayas , Hipotálamo/efectos de los fármacos , Hipotálamo/enzimología , Masculino , Potasio/farmacología , Prolactina/farmacología , Receptores de GABA/efectos de los fármacos , Estaciones del Año , Estimulación Química , Factores de Tiempo , Ácido gamma-Aminobutírico/efectos de los fármacosRESUMEN
The morpho-functional indexes of ejaculates from 35 healthy and 145 infertile men were studied. The depression of functional sperm activity was found in the majority of infertile men. Phospholipid analysis of whole ejaculates from 12 healthy and 35 infertile subjects was performed. It was shown that inorganic phosphorus of total phospholipids decreased in ejaculates of men with secretory infertility. Lyso-phosphatidyl choline (LPC) was not detected in ejaculates of men with relative infertility. The amount of lyso-phosphatidyl ethanolamine (lyso-PE) and sphingomyelin in whole ejaculates of those patients decreased. The levels of LPC and lyso-PE decreased in ejaculates of persons with associated infertility. The level of phosphatidyl inositol, LPC and PE decreased in ejaculates of men with secretory infertility. The gas-lipid chromatography analysis of fatty acids composition of whole ejaculates of the infertile men showed significant changes in quantity of fatty acids. The quantity of docosahexaenoic acid in ejaculates of infertile men decreased and positively correlated with motility of spermatozoa.