RESUMEN
Currently, the efficacy of drug therapy for post-traumatic stress disorder or PTSD leaves much to be desired, making nutraceutical support a promising avenue for treatment. Recent research has identified the protective effects of resveratrol in PTSD. Here, we tested the behavioral and neurobiological effects of combining cheese consumption with resveratrol supplements in an experimental PTSD model. Using the elevated plus maze test, we observed that cheese intake resulted in a shift from anxiety-like behavior to depressive behavior, evident in increased freezing acts. However, no significant changes in the anxiety index value were observed. Interestingly, supplementation with cheese and resveratrol only led to the elimination of freezing behavior in half of the PTSD rats. We further segregated the rats into two groups based on freezing behavior: Freezing+ and Freezing0 phenotypes. Resveratrol ameliorated the abnormalities in Monoamine Oxidize -A and Brain-Derived Neurotrophic Factor gene expression in the hippocampus, but only in the Freezing0 rats. Moreover, a negative correlation was found between the number of freezing acts and the levels of Monoamine Oxidize-A and Brain-Derived Neurotrophic Factor mRNAs in the hippocampus. The study results show promise for resveratrol supplementation in PTSD treatment. Further research is warranted to better understand the underlying mechanisms and optimize the potential benefits of resveratrol supplementation for PTSD.
Asunto(s)
Queso , Trastornos por Estrés Postraumático , Animales , Ratas , Trastornos por Estrés Postraumático/tratamiento farmacológico , Factor Neurotrófico Derivado del Encéfalo/genética , Resveratrol/farmacología , Resveratrol/uso terapéutico , Aminas , Suplementos DietéticosRESUMEN
Variations in anxiety-related behavior are associated with individual allostatic set-points in chronically stressed rats. Actively offensive rats with the externalizing indicators of sniffling and climbing the stimulus and material tearing during 10 days of predator scent stress had reduced plasma corticosterone, increased striatal glutamate metabolites, and increased adrenal 11-dehydrocorticosterone content compared to passively defensive rats with the internalizing indicators of freezing and grooming, as well as to controls without any behavioral changes. These findings suggest that rats that display active offensive activity in response to stress develop anxiety associated with decreased allostatic set-points and increased resistance to stress.
Asunto(s)
Ansiedad/metabolismo , Ansiedad/psicología , Cuerpo Estriado/metabolismo , Ácido Glutámico/metabolismo , Hipotálamo/metabolismo , Sistema Límbico/metabolismo , Sistema Hipófiso-Suprarrenal/metabolismo , Estrés Psicológico , Animales , Ansiedad/diagnóstico por imagen , Ansiedad/etiología , Conducta Animal , Biomarcadores , Cuerpo Estriado/fisiopatología , Modelos Animales de Enfermedad , Hormonas/metabolismo , Imagen por Resonancia Magnética , Masculino , Aprendizaje por Laberinto , Ratas , Análisis Espectral , Estrés FisiológicoRESUMEN
The aim of this study was to investigate the effect of chronic predator scent stress (PSS) on monoamine levels in rat thalamus and hypothalamus. Rats were exposed to the PSS (sand containing cat urine) for ten minutes daily for ten days. Control animals were exposed to the sand containing clean water. Fifteen days later, rats' behavior and thalamic and hypothalamic levels of monoamines were analyzed. PSS rats had elevated anxiety, increased thalamic serotonin and decreased hypothalamic dopamine concentrations. This decrease in hypothalamic dopamine may explain, at least in part, lowered corticosterone levels observed in PSS animals in our previous studies.