RESUMEN
BACKGROUND: In areas of high exposure to grass pollen, allergic patients are frequently sensitized to profilin, and some experience severe profilin-mediated food-induced reactions. This specific population of patients is ideal to study the relationship between respiratory and food allergies. OBJECTIVE: We sought to determine the role of oral mucosal epithelial barrier integrity in profilin-mediated allergic reactions. METHODS: Thirty-eight patients with profilin allergy stratified into mild or severe according to their clinical history and response to a profilin challenge test and 6 nonallergic subjects were recruited. Oral mucosal biopsies were used for measurement of CD11c, CD3, CD4, tryptase, claudin-1, occludin, E-cadherin, and vascular endothelial growth factor A levels; Masson trichrome staining; and POSTN, IL33, TPSAB, TPSB, and CMA gene expression analysis by using quantitative RT-PCR. Blood samples were used for basophil activation tests. RESULTS: Distinct features of the group with severe allergy included the following: (1) impaired epithelial integrity with reduced expression of claudin-1, occludin, and E-cadherin and decreased numbers of epithelial cells, which is indicative of acanthosis, higher collagen deposition, and angiogenesis; (2) inflammatory immune response in the mucosa, with an increased number of CD11c+ and CD4+ infiltrates and increased expression of the cytokine genes POSTN and IL33; and (3) a 10-fold increased sensitivity of basophils to profilin. CONCLUSIONS: Patients with profilin allergy present with significant damage to the oral mucosal epithelial barrier, which might allow profilin penetration into the oral mucosa and induction of local inflammation. Additionally, severely allergic patients presented with increased sensitivity of effector cells.
Asunto(s)
Basófilos/inmunología , Hipersensibilidad a los Alimentos/inmunología , Mucosa Bucal/patología , Hipersensibilidad Respiratoria/inmunología , Uniones Estrechas/patología , Adulto , Alérgenos/inmunología , Claudina-1/genética , Claudina-1/metabolismo , Reacciones Cruzadas , Femenino , Humanos , Inmunoglobulina E/metabolismo , Masculino , Persona de Mediana Edad , Poaceae/inmunología , Polen/inmunología , Profilinas/inmunología , Adulto JovenRESUMEN
Contiene la importancia de la Historia Clínica Perinatal Simplificada y es planteada la necesidad de crear un sistema de información adecuado, preciso y de fácil manejo para conocer más a fondo dicho requisito, surgiendo de esta manera, un Sistema Informático Perinatal. Los apartados del documento son: Introducción I. Material y método II. Antecedentes III. Resultados IV. Conclusiones V. Bibliografía. Se incluyen dos cuadros con las principales características entre la primera consulta prenatal y el índice de marginación en unidades de primer nivel y en hospitales