RESUMEN
The present study evaluated whether the oxidative stress caused by antimicrobial photodynamic therapy (aPDT) affects the expression of C. albicans genes related to adhesion and biofilm formation (ALS1 and HPW1) and oxidative stress response (CAP1, CAT1, and SOD1). The aPDT was mediated by two photosensitizing agents (PSs) Photodithazine® (PDZ at 100 and 200â¯mg/L) or Curcumin (CUR at 40 and 80 µM) and LED (37.5â¯J/cm2 or 50â¯J/cm2). The quantification of the expression was performed by Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR) using specific primers for the target genes. The data were analyzed by Analysis of Variance (αâ¯=â¯0.05), followed by Tukey's post-test. It was observed reduction in the expression of ALS1, HWP1, CAP1, CAT1, and SOD1 when aPDT was performed using 200â¯mg/L PDZ and 80 µM CUR associated to LED (37.7 and 50â¯J/cm2, respectively) and using 100â¯mg/L PDZ and 40 µM CUR with LED of 50â¯J/cm2 (versus control). Also, the expression of CAP1 and SOD1 genes was reduced after aPDT using 100â¯mg/L PDZ and LED of 37.5â¯J/cm2. There was a significant reduction in the expression of genes HWP1, CAP1, and SOD1 after aPDT using 40 µM CUR and 37.5â¯J/cm2 (versus the control group). The application of LED only at 37.5 and 50â¯J/cm2 promoted down-regulation of ALS1, CAP1, CAT1, and SOD1 genes (versus the control group). Therefore, aPDT mediated by LED -associated PSs PDZ and CUR promoted a reduction in the expression of the five C. albicans genes evaluated.