Acute Effects of Whole-Body Electromyostimulation during a Single Maximal Strength Training Session.

Whole-body electromyostimulation (WB-EMS) training is effective in improving training adaptation. However, WB-EMS may have side effects and contraindications that can lead to excessive muscle damage and physiological impairment. This randomized crossover study aimed to analyze the acute effects of WB-EMS on muscle damage, autonomic modulation and performance during a single maximal strength session in physically active participants. Twenty healthy and physically active participants randomly performed three maximal strength training sessions (90% 1RM) consisting of bench presses and squat exercises, with a continuous stimulus, a coordinated stimulus with concentric and eccentric phases, and without WB-EMS. Data showed no significant differences between the trials for muscle damage (blood creatine kinase levels), lactate blood levels and performance after exercise. Likewise, the heart rate, blood oxygen saturation and the rate of perceived exertion were similar during exercise between trials. The heart rate variability analysis also showed a similar autonomic response among the trials. Training with WB-EMS seemed to be safe at the observed time intervals while offering a stimulus similar to regular training in physically active participants, regardless of the delivery of the electrical stimuli. More studies are needed to assess the effectiveness of WB-EMS in improving exercise adaptations during training programs.

Evaluating the effect of CFH-12® and Phoslock® on phosphorus dynamics during anoxia and resuspension in shallow eutrophic lakes.

Laboratory experiments with intact sediment cores from a hypertrophic very windy exposed shallow lake were conducted to assess the combined effect of anoxia and sediment resuspension on phosphorus (P) dynamics after adding different P adsorbents (CFH-12® and Phoslock®). In this study we hypothesize that the addition of geoengineering materials will increase P retention in the sediment even at the worst physic-chemical conditions such as anoxia and sediment resuspension. Both adsorbents significantly reduced the P release from the sediments after a 54 days-anoxic incubation period (CFH-12® by 85% and Phoslock® by 98%) and even after resuspension events (CFH-12® by 84% and Phoslock® by 88%), indicating that both adsorbents are suitable P inactivating agents for restoring shallow eutrophicated lakes under such circumstances. CFH-12® did not release dissolved Fe to the water column neither after the anoxic period nor after resuspension events compared to Control (no adsorbents addition). The La concentration was significantly higher in Phoslock® (3.5-5.7 µg L-1) than in Control at all sampling days but it was not affected by resuspension. The high efficiency in P removal under anoxia and resuspension, the low risk of toxicity and the high maximum adsorption capacity makes CFH-12® a promising adsorbent for lake restoration. Nevertheless, further research about the influence of other factors (i.e. pH, alkalinity, interfering substances or strict anoxia) on the performance of CFH-12® is needed.

UV-initiated crosslinking of electrospun chitosan/poly(ethylene oxide) nanofibers doped with ZnO-nanoparticles: development of antibacterial nanofibrous hydrogel.

UV-initiated crosslinking of electrospun poly(ethylene) oxide (PEO)/chitosan (CS) nanofibers doped with zinc oxide nanoparticles (ZnO-NPs) was performed using pentaerythritol triaclyrate (PETA) as the photoinitiator and crosslinker agent. The influence of the addition of PETA to the PEO/CS diameter and crosslinking of nanofibers was evaluated. The effect of irradiation time on the morphology and swelling properties of the crosslinked nanofibers were investigated. For ZnO-NPs, the minimum inhibitory concentrations were found at 1 mg/mL, and the minimum bactericidal concentrations at 2 mg/mL for all the strains tested. The nanofibrous hydrogel antibacterial effect was tested. This material enters the realm of fibrous hydrogels which have potential use in several applications as in the biomedical area. SUPPLEMENTARY MATERIAL: The supplementary material for this article can be found at 10.1557/mrc.2020.74.

Análise bioeconômica do farelo de arroz na recria de bezerras de corte em azevém / Bioeconomics analyses of rice brain use for reaning of beef heifers on Italian ryegrass pasture

Objetivou-se avaliar a economicidade, o desempenho produtivo e o desenvolvimento das estruturas corporais relacionadas ao trato reprodutivo de bezerras de corte mantidas exclusivamente em pastagem de azevém (Lolium multiflorum Lam.) ou em pastagem de azevém recebendo 0,5 e 1,0% do peso corporal (PC) de farelo de arroz integral (FAI) como suplemento. O método de pastejo foi o rotativo, e o intervalo entre pastejos foi estabelecido considerando-se a soma térmica necessária para a emissão de 1,5 folha de azevém (187,5ºC). O delineamento experimental foi o inteiramente ao acaso, com medidas repetidas no tempo. O ganho médio diário das bezerras não diferiu entre os sistemas alimentares. A taxa de lotação e o ganho de peso por área foram maiores quando as bezerras receberam 1,0% do PC de farelo de arroz integral. A maior margem bruta foi observada quando as bezerras receberam 0,5% de FAI. O custo variável obtido para os diferentes sistemas alimentares apresentou comportamento crescente em função dos níveis de FAI, superando o uso exclusivo do azevém em 49,4% e 81,8%. O peso corporal final, o escore de condição corporal e o escore do trato reprodutivo não diferiram entre os sistemas alimentares. Considerando-se um animal adulto com peso corporal médio de 450kg, as bezerras ao início do experimento apresentaram 34% do peso corporal adulto e peso corporal final médio de 56% do peso adulto. A utilização de níveis de farelo de arroz integral (FAI) na recria de bezerras de corte sob pastejo rotativo em azevém não modifica o ganho médio diário e o escore do trato reprodutivo. O fornecimento de 1% de FAI mostrou efeito substitutivo, proporcionando um incremento de 31,2% na taxa de lotação e de 38,3% no ganho por área. O sistema alimentar AZ0,5 permitiu uma maior margem bruta; já o retorno financeiro direto foi positivo em todos os sistemas alimentares, com melhor retorno calculado para o uso exclusivo do azevém.(AU)

The experiment was carried out with the objective of evaluating the gross margin, the productive and reproductive performance of beef heifers kept exclusively on ryegrass pasture (Lolium multiflorum Lam.) or ryegrass receiving 0.5 and 1.0% of body weight (BW) of whole rice bran as supplement. The grazing method was the rotational and the interval between grazings was set by the thermal sum required for the appearing of 1.5 ryegrass leaf (187.5ºC). The experimental design was completely randomized with repeated measures. Heifers average daily gain did not differ between the feeding systems. The stocking rate and weight gain per area were higher when heifers received 1.0% BW of whole rice bran. The higher gross margin was observed when heifers received 0.5% BW. The variable cost obtained for the different feeding systems showed increasing behavior depending on the WRB levels, surpassing the exclusive use of ryegrass in 49.4% and 81.8%. The final body weight, body condition score and reproductive tract score did not differ between the feeding systems. Considering an adult animal with an average body weight of 450kg, the heifers at the beginning of the experiment showed 34% of mature body weight and final average body weight of 56% of adult weight. The use of rice bran levels (FAZ) in the rearing of beef heifers under rotational grazing on ryegrass does not change the average daily gain and the reproductive tract score. The supply of 1% FAI showed substitutive effect, providing an increase in capacity of 31,2% and 38.3% rate of the gain per area. The food system AZ0,5 allowed a higher gross margin, as the economic return was positive in all food systems, with better return calculated for the exclusive use of ryegrass.(AU)

Experimental models in Chagas disease: a review of the methodologies applied for screening compounds against Trypanosoma cruzi.

One of the main problems of Chagas disease (CD), the parasitic infection caused by Trypanosoma cruzi, is the lack of a completely satisfactory treatment, which is currently based on two old nitroheterocyclic drugs (i.e., nifurtimox and benznidazole) that show important limitations for treating patients. In this context, many laboratories look for alternative therapies potentially applicable to the treatment, and therefore, research in CD chemotherapy works in the design of experimental protocols for detecting molecules with activity against T. cruzi. Phenotypic assays are considered the most valuable strategy for screening these antiparasitic compounds. Among them, in vitro experiments are the first step to test potential anti-T. cruzi drugs directly on the different parasite forms (i.e., epimastigotes, trypomastigotes, and amastigotes) and to detect cytotoxicity. Once the putative trypanocidal drug has been identified in vitro, it must be moved to in vivo models of T. cruzi infection, to explore (i) acute toxicity, (ii) efficacy during the acute infection, and (iii) efficacy in the chronic disease. Moreover, in silico approaches for predicting activity have emerged as a supporting tool for drug screening procedures. Accordingly, this work reviews those in vitro, in vivo, and in silico methods that have been routinely applied during the last decades, aiming to discover trypanocidal compounds that contribute to developing more effective CD treatments.

Determining major factors controlling phosphorus removal by promising adsorbents used for lake restoration: A linear mixed model approach.

Phosphorus (P) removal from lake/drainage waters by novel adsorbents may be affected by competitive substances naturally present in the aqueous media. Up to date, the effect of interfering substances has been studied basically on simple matrices (single-factor effects) or by applying basic statistical approaches when using natural lake water. In this study, we determined major factors controlling P removal efficiency in 20 aquatic ecosystems in the southeast Spain by using linear mixed models (LMMs). Two non-magnetic -CFH-12® and Phoslock®- and two magnetic materials -hydrous lanthanum oxide loaded silica-coated magnetite (Fe-Si-La) and commercial zero-valent iron particles (FeHQ)- were tested to remove P at two adsorbent dosages. Results showed that the type of adsorbent, the adsorbent dosage and color of water (indicative of humic substances) are major factors controlling P removal efficiency. Differences in physico-chemical properties (i.e. surface charge or specific surface), composition and structure explain differences in maximum P adsorption capacity and performance of the adsorbents when competitive ions are present. The highest P removal efficiency, independently on whether the adsorbent dosage was low or high, were 85-100% for Phoslock and CFH-12®, 70-100% for Fe-Si-La and 0-15% for FeHQ. The low dosage of FeHQ, compared to previous studies, explained its low P removal efficiency. Although non-magnetic materials were the most efficient, magnetic adsorbents (especially Fe-Si-La) could be proposed for P removal as they can be recovered along with P and be reused, potentially making them more profitable in a long-term period.

Victimisation through bullyi8ng and cyberbullying: emotional intelligence, severity of victimisation and technology use in different types of victims / Victimización de bullying, cyberbullying y ambas: inteligencia emocional, gravedad de victimización y uso de tecnologías en diferentes tipos de víctimas

BACKGROUND: Bullying and cyberbullying are global public health problems. However, very few studies described prevalence, similarities and differences among face-to-face victims, cybervictims and students who are victimised through both bullying and cyberbullying. This study was conducted to describe these different patterns of victimisation and severity of victimisation, emotional intelligence and technology use in different types of victims. METHOD: A total number of 2,139 secondary school students from 22 schools, randomly selected from all provinces of Andalusia, Spain, participated in this study. Information about bullying, cyberbullying, social networking sites use and perceived emotional intelligence was collected. RESULTS: Face-to-face victimisation only is the most common type of victimisation followed by mixed victimisation. Cybervictimisation only is rare. Mixed victims score higher in severity of bullying and present higher emotional attention than face-to-face victims. CONCLUSIONS: Most victims of cyberbullying are also face-to-face victims. Holistic approach that focuses on different problems at the same time seems to be needed to tackle these behaviours

ANTECEDENTES: el bullying es un problema de salud pública mundial. Sin embargo, muy pocos estudios describieron la prevalencia, las similitudes y las diferencias entre las víctimas cara a cara, cibervíctimas y estudiantes que han sido victimizados de ambas maneras. Este estudio se ha llevado a cabo para describir estos diferentes patrones de victimización y la gravedad de victimización, la inteligencia emocional y el uso de las tecnologías en diferentes tipos de víctimas. MÉTODO: en este estudio participaron 2.193 estudiantes de 22 escuelas, seleccionadas aleatoriamente de todas las provincias de Andalucía, España. Se recogió información sobre bullying, cyberbullying, uso de redes sociales e inteligencia emocional percibida. RESULTADOS: la victimización cara a cara es el tipo más común de victimización seguida de victimización mixta. Cibervictimización sola es poco común. Las víctimas mixtas muestran puntuación más alta en la gravedad de la victimización cara a cara y una mayor atención emocional que las víctimas cara a cara. CONCLUSIONES: la mayoría de las cibervíctimas son también víctimas cara a cara. Un enfoque holístico centrado en diferentes problemas a la vez parece necesario para erradicar estos problemas

Victimisation through bullying and cyberbullying: Emotional intelligence, severity of victimisation and technology use in different types of victims.

BACKGROUND: Bullying and cyberbullying are global public health problems. However, very few studies described prevalence, similarities and differences among face-to-face victims, cybervictims and students who are victimised through both bullying and cyberbullying. This study was conducted to describe these different patterns of victimisation and severity of victimisation, emotional intelligence and technology use in different types of victims. METHOD: A total number of 2,139 secondary school students from 22 schools, randomly selected from all provinces of Andalusia, Spain, participated in this study. Information about bullying, cyberbullying, social networking sites use and perceived emotional intelligence was collected. RESULTS: Face-to-face victimisation only is the most common type of victimisation followed by mixed victimisation. Cybervictimisation only is rare. Mixed victims score higher in severity of bullying and present higher emotional attention than face-to-face victims. CONCLUSIONS: Most victims of cyberbullying are also face-to-face victims. Holistic approach that focuses on different problems at the same time seems to be needed to tackle these behaviours.

Insemination extender supplementation with bestatin and EDTA has no effect on rabbit reproductive performance.

The addition of aminopeptidase inhibitors (AMIs) to rabbit semen extenders could be a solution to decrease the hormone degradation (GnRH) by the aminopeptidases existing in the seminal plasma. Therefore, the quantity of GnRH needed to induce ovulation in doe would be comparable with the amount administered intramuscularly (i.m.). This study was conducted to evaluate the effects of two AMIs (bestatin and EDTA) on rabbit semen quality parameters, ß nerve growth factor (ß-NGF) degradation and reproductive performance after artificial insemination. Results showed that seminal quality was not affected by the incubation with AMIs; the values of motility, acrosome integrity and sperm viability were not significantly different between the AMIs and the control groups (positive i.m. and negative intravaginally without AMIs). In addition, the aminopeptidase activity of seminal plasma was inhibited in a 55.5% by the AMIs as well as ß-NGF degradation. On the other hand, regarding the effect of AMIs on reproductive performance, our results showed that the presence of bestatin and EDTA did neither affect fertility (85.3 vs. 88.6%), nor the prolificacy rate (10.12 vs. 10.51 kits per delivery), comparing AMIs group to positive control group, respectively. We conclude that the addition of specific AMIs in the rabbit semen extender has no effect on reproductive performance. Therefore, due to the fact that AMIs inhibit part of the aminopeptidase activity that degrades the GnRH analogue and ß-NGF, they could be used to develop new extenders with less hormone concentration.

Forage intake and performance of heifers fed rice bran on Alexandergrass pasture / Ingestão de forragem e performance de bezerros alimentados com farelo de arroz integral em pasto de papuã

he study was carried out to evaluate the forage intake and performance of beef heifers from 15 to 18 months of age, grazing on Alexandergrass (Urochloaplantaginea (Link) Hitch) fed rice bran (RB; 0, 0.5 and 1.0% body weight (BW)). The experiment consisted of a completely randomized design with repeated measurement arrangement. Rice bran increased the total dry matter (DM) intake, total crude protein intake and total digestible nutrients intake. Heifers that received 1.0% BW of RB reduced forage DM intake, increasing by 18% stocking rate (SR) and by 27% BW gain per area in comparison with the system exclusively on pasture. In the system in which the heifers were fed 0.5% BW of RB, SR and gain per area were similar to other systems. In this feeding system, we observed average daily gain 18% higher than when heifers were exclusively on pasture and gain similar to that observed when using 1.0% BW of RB. In Alexandergrass pasture it is recommended to supply 0.5% BW of RB for beef heifers from 15 to 18 months of age, to promote better productive responses compared to heifers exclusively on pasture and similar to those observed when supplying 1.0% BW of RB.(AU)

O presente trabalho foi desenvolvido com o objetivo de avaliar o consumo de forragem e o desempenho de novilhas de corte dos 15 aos 18 meses de idade, em pastejo em papuã (Urochloaplantaginea(Link) Hitch), recebendo farelo de arroz integral (FAI) como suplemento (0, 0,5 e 1,0% do peso corporal (PC)). O delineamento experimental foi o inteiramente ao acaso, com medidas repetidas no tempo. O uso de FAI aumentou o consumo total de matéria seca (MS), o consumo total de proteína bruta e de nutrientes digestíveis totais em relação às novilhas exclusivamente em pastejo. As novilhas que receberam 1,0% do PC de FAI reduziram o consumo de MS da forragem, aumentando em 18% a taxa de lotação (TxLot) e em 27% o ganho de PC por hectare (GPA) em comparação com o sistema exclusivamente a pasto. No sistema em que as novilhas receberam 0,5% do PC de FAI, a TxLot e o GPA foram similares aos demais sistemas. Nesse sistema alimentar, foi observado GDM 18% superior em relação às novilhas exclusivamente em pastejo e ganho similar ao uso de 1,0% do PC de FAI. Em pastagem de papuã, é recomendado o uso de 0,5% do PC de FAI para novilhas de corte dos 15 aos 18 meses de idade, por promover respostas produtivas superiores em relação às novilhas exclusivamente em pastejo e semelhantes às observadas quando fornecido 1,0% do PC de FAI.(AU)

Effects of the inclusion of a Bacillus direct-fed microbial on performance parameters, bone quality, recovered gut microflora, and intestinal morphology in broilers consuming a grower diet containing corn distillers dried grains with solubles.

Distillers dried grains with solubles (DDGS) have increasingly been used in poultry diets as a consequence of rising grain costs. Some, but not all, sources of DDGS have a variable compositional value, and a high inclusion of this by-product could be considered a risk factor for presentation of enteric diseases. Presently, 2 experiments were conducted using a starter corn-soybean diet (zero to 7 d) and a corn-DDGS-soybean grower diet (8 to 28 d) with or without inclusion of a Bacillus-direct-fed microbial (DFM). In both experiments, day-of-hatch chicks were randomly assigned to 2 different groups: control group without DFM or Bacillus-DFM group, containing 106 spores/g of feed. In each experiment, 8 pens of 20 chicks (n = 160/group) were used. Performance parameters of BW, BW gain (BWG), feed intake (FI), and feed conversion (FCR) were evaluated in each growth phase. Additionally, in experiment 2, intestinal samples were collected to determine duodenal and ileal morphology (n = 8/group), as well as the microbiota population of total lactic acid bacteria (TLAB), total Gram-negative bacteria (TGNB), and total anaerobic bacteria (TAB) on d 28 (n = 16/group). Furthermore, both tibias were evaluated for bone strength and bone composition (n = 16/group). In both experiments BW, BWG, and FCR were improved by the DFM when compared to the control group (P < 0.05). In experiment 2, chickens supplemented with the DFM had less TGNB in the foregut intestinal segment and higher TLAB counts in both foregut and hindgut sections (P < 0.05). In addition significant increases in tibia breaking strength and bone mineralization were observed in the DFM group when compared with the control. In the case of intestinal morphology, DFM dietary inclusion increased villus height (VH), villus width, villus area, muscular thickness, and the VH to crypt depth ratio (VH:CD) in both duodenum and ileum sections. Results of the present study suggest that consumption of a selected Bacillus-DFM producing a variable set of enzymes could contribute to enhanced performance, intestinal microbial balance, and bone quality in broiler chickens consuming a grower diet that contains corn-DDGS.

Identification, occurrence and activity of quinazoline alkaloids in Peganum harmala.

Peganum harmala L. is a medicinal plant from the Mediterranean region and Asia currently used for recreative psychoactive purposes (Ayahuasca analogue), and increasingly involved in toxic cases. Its psychopharmacological and toxicological properties are attributed to quinazoline and ß-carboline alkaloids. In this work three major quinazoline alkaloids were isolated from P. harmala extracts and characterized as peganine (vasicine), deoxypeganine (deoxyvasicine) and a novel compound identified by HPLC-DAD-MS and NMR as peganine ß-d-glucopyranosyl-(1 â†’ 6)-ß-d-glucopyranoside (peganine glycoside). Peganine appeared in flowers and leaves in high levels; high amounts of deoxypeganine and peganine were found in immature and green fruits whereas peganine and peganine glycoside accumulated in high amount in dry seeds reaching up to 1 and 3.9% (w/w), respectively. Roots and stems contained low amount of quinazolines. Seeds extracts containing both quinazoline and ß-carboline alkaloids potently inhibited human monoamine oxidase (MAO)-A. However, quinazoline alkaloids did not contribute to MAO inhibition that was due to ß-carbolines, suggesting that MAO-related psychoactive or toxic actions do not arise from quinazolines. Quinazoline alkaloids were poor radical scavengers in the ABTS assay whereas seed extracts had good activity. Quinazoline alkaloids are known to exert bronchodilator and abortifacient actions, and could contribute to such effects reported in P. harmala.

Synthesis and characterization of magnetic chitosan microspheres as low-density and low-biotoxicity adsorbents for lake restoration.

We propose a novel magnetic adsorbent for optimal Phosphorus (P) removal from the upper sediment layers. For this aim, magnetic chitosan microparticles were prepared using a reverse-phase suspension cross-linking technique. The resulting particles and suspensions were characterized using scanning electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, magnetometry, thermogravimetric analysis, electrophoretic mobility and turbidity measurements. The hybrids are multicore particles consisting of well dispersed magnetite nanoparticles (approx. 10% w/w) homogeneously distributed within the biopolymer matrix. These microparticles can be easily separated from the water column and sediment using magnetic field gradients. Their P adsorption capacity is evaluated in batch conditions resulting in a maximum P adsorption capacity of ML = 4.84 mg g-1 at pH = 7. We demonstrate that these particles are excellent candidates to remove P from water column and also P mobile from the upper sediment layers due to two main reasons: they sediment slower and present lower potential toxicity (due to a their larger size) than conventional iron/iron oxide microparticles previously proposed for lake restoration.

Magnetic microparticles as a new tool for lake restoration: A microcosm experiment for evaluating the impact on phosphorus fluxes and sedimentary phosphorus pools.

In the last decades, magnetic particles (MPs) as adsorbents have gained special attention due to their high adsorption capacity and the possibility of recovering them by applying a magnetic separation gradient. For the first time MPs have been tested as P adsorbents in a microcosm experiment in a context of lake restoration. MPs were added to sediment cores from a hypertrophic lake, at Fe:PMobile molar ratio of 285:1 and 560:1 under both, oxic and anoxic conditions. We have found that, under anoxic conditions (anoxic), MPs are able to reduce P release rate from the sediment to the overlying water and to reduce sedimentary PMobile concentration (a 22-25% reduction within 0-4 cm depth compared to controls). Under oxic conditions, the addition of MPs do not affect P fluxes across the sediment and water interface since the lake sediment is naturally rich in iron oxides. However a measured reduction in sedimentary PMobile concentration (12-16% reduction in 0-10 cm depth) contributes to a potential reduction in long-term P efflux.

Late sodium current block for drug-induced long QT syndrome: Results from a prospective clinical trial.

Drug-induced long QT syndrome has resulted in many drugs being withdrawn from the market. At the same time, the current regulatory paradigm for screening new drugs causing long QT syndrome is preventing drugs from reaching the market, sometimes inappropriately. In this study, we report the results of a first-of-a-kind clinical trial studying late sodium (mexiletine and lidocaine) and calcium (diltiazem) current blocking drugs to counteract the effects of hERG potassium channel blocking drugs (dofetilide and moxifloxacin). We demonstrate that both mexiletine and lidocaine substantially reduce heart-rate corrected QT (QTc) prolongation from dofetilide by 20 ms. Furthermore, all QTc shortening occurs in the heart-rate corrected J-Tpeak (J-Tpeak c) interval, the biomarker we identified as a sign of late sodium current block. This clinical trial demonstrates that late sodium blocking drugs can substantially reduce QTc prolongation from hERG potassium channel block and assessment of J-Tpeak c may add value beyond only assessing QTc.

Evaluation of a Bacillus direct-fed microbial candidate on digesta viscosity, bacterial translocation, microbiota composition and bone mineralisation in broiler chickens fed on a rye-based diet.

1. The effects of the dietary inclusion of a Bacillus-based direct-fed microbial (DFM) candidate on digesta viscosity, bacterial translocation, microbiota composition and bone mineralisation were evaluated in broilers consuming rye-based diets. 2. In the present study, control mash rye-based diets (CON) or Bacillus-DFM supplemented diets (TRT) were administered ad libitum to male broilers in three independent experiments. 3. In Experiments 1 and 2 (n = 25/group), liver samples were taken to evaluate bacterial translocation, digesta samples were used for viscosity measurements and the intestinal microbial flora was evaluated from different intestinal sections to enumerate total recovered gram-negative bacteria (TGB), lactic acid bacteria (LAB) and anaerobic bacteria (TAB). Additionally, both tibias were removed for assessment of bone quality. 4. In Experiment 3, each experimental group had 8 replicates of 20 chickens (n = 160/group). Weekly, body weight (BW), feed intake (FI) and feed conversion ratio (FCR) were evaluated. At d 28-of-age, samples were taken to determine bacterial translocation, digesta viscosity and bone quality characteristics. 5. In all experiments, consumption of Bacillus-DFM reduced bacterial translocation to the liver and digesta viscosity. Additionally, DFM supplementation improved BW, bone quality measurements and FCR. Moreover, chickens fed on the Bacillus-DFM diet in Experiments 1 and 2 showed a significant reduction in the number of gram-negative and anaerobic bacteria in the duodenal content compared to control. 6. In summary, chickens fed on a rye-based diet without DFM inclusion showed an increase in bacterial translocation and digesta viscosity, accompanied by reduced performance and bone quality variables relative to the Bacillus-DFM candidate group. Hence, incorporation into the feed of a selected DFM ameliorated the adverse anti-nutritional effects related to utilisation of rye-based diets in broilers chickens.

The effect of five Taraxacum species on in vitro and in vivo antioxidant and antiproliferative activity.

Plants belonging to the genus Taraxacum are considered a nutritious food, being consumed raw or cooked. Additionally, these plants have long been used in folk medicine due to their choleretic, diuretic, antitumor, antioxidant, antiinflammatory, and hepatoprotective properties. This genus, with its complex taxonomy, includes several species that are difficult to distinguish. Its traditional use must be related not only to T. officinale F.H. Wigg., the most studied species, but also to others. The aim of this work is to compare five different common South European species of Taraxacum (T. obovatum (Willd.) DC., T. marginellum H. Lindb., T. hispanicum H. Lindb., T. lambinonii Soest and T. lacistrum Sahlin), in order to find differences between antioxidant and cytotoxic activities among them. Dissimilarities between species in LC/MS patterns, in in vitro and intracellular antioxidant activity and also in the cytotoxicity assay were found. T. marginellum was the most efficient extract reducing intracellular ROS levels although in in vitro assays, T. obovatum was the best free radical scavenger. A relevant cytotoxic effect was found in T. lacistrum extract over HeLa and HepG2 cell lines.

In vitro leishmanicidal activity of 1,3-disubstituted 5-nitroindazoles.

The antiprotozoal activity of some indazole-derived amines (2, 3, 5-8) as well as that of some simple structurally related 3-alkoxy-1-alkyl-5-nitroindazoles (1, 4) against promastigote and amastigote forms of Leishmania infantum and Leishmania braziliensis is reported. In some cases, these compounds showed in vitro activities against the different morphological forms of Leishmania similar to or higher than those of the reference drug glucantime; this fact, along with low unspecific cytotoxicities against macrophages shown by some of them, led to good selectivity indexes (SI). The high efficiency of some 5-nitroindazoles against the mentioned protozoa was confirmed by further in vitro studies on infection rates. Complementary analyses by (1)H NMR of the changes on the metabolites excreted by parasites after treatment with the more active indazole derivatives in many cases showed the decreased excretion of succinate and increased levels of acetate, lactate and alanine, as well as, in some cases, the appearance of glycine and pyruvate as new metabolites. Damage caused by indazoles at the glycosomal or mitochondrial level are consistent with these metabolic changes as well as with the huge ultrastructural alterations observed by transmission electron microscopy (TEM), especially affecting the mitochondria and other cytoplasmic organelles.

A sequential procedure for rapid and accurate identification of putative trichomonacidal agents.

In the current report, a sequential step-wise methodology based on in silico, in vitro and in vivo experimental procedures for the prompt detection of potential trichomonacidal drugs is proposed. A combinatorial of 12 QSAR (Quantitative Structure-Activity Relationship) models based on Linear Discrimination Analysis (LDA) are suggested for the rational identification of new trichomonacidal drugs from virtual screening of in house chemical libraries and drug databases. Subsequently, compounds selected as potential anti-trichomonas are screened in vitro against Trichomonas vaginalis. Finally, molecules with specific trichomonacidal activity are evaluated in vivo. Herein, different molecules were exposed to the proposed methodology. Firstly, the agents were virtually screened and two of the eight molecules (G-1 and dimetridazole) were classified as trichomonacidals by the 12 models. Subsequently both drugs were proved in vitro and in vivo following the workflow procedure. Although a remarkable in vitro activity was observed in both cases, dimetridazole achieved higher MIC100 activity than metronidazole against the resistant isolate. Furthermore, the in vivo models showed a remarkable reduction of lesions of more than 55% in both compounds. These observations support the current flowchart screening and suggest the use of dimetridazole as a promising drug-like scaffold for novel therapeutic alternatives against T. vaginalis resistant infections.