RESUMEN
Antimicrobial activity of dichloromethane and ethanol extracts and five compounds: pinostrobin (I), pinocembrin (II), tectochrysin (III), galangin 3-methyl ether (IV) and tiliroside (V) isolated from Lychnophora markgravii aerial parts against fifteen microorganisms was determined. The structures of these compounds were elucidated based on ESI-MS and NMR spectroscopic data. Both extracts showed antimicrobial activity against several tested microorganisms. Pinostrobin, tectochrysin and galangin 3-methyl ether showed the strongest antibacterial and antifungal effects.
Asunto(s)
Antibacterianos/aislamiento & purificación , Antifúngicos/aislamiento & purificación , Asteraceae/química , Extractos Vegetales/química , Antibacterianos/química , Antibacterianos/farmacología , Antifúngicos/química , Antifúngicos/farmacología , Flavanonas/análisis , Flavanonas/química , Flavonoides/análisis , Flavonoides/química , Pruebas de Sensibilidad Microbiana , Estructura MolecularRESUMEN
Two new sesquiterpene lactones 8alpha-(2',3'-dihydroxy-2'-methylbutanoyl)-15-desoxygoyazensolide and 16alpha-(1',2'-dihydroxy-1'-methylpropyl)-eremantholide have been identified as constituents of Eremanthus argenteus aerial parts. In addition, two known sesquiterpene lactones and three flavonoids were isolated. Their structures were established on the basis of spectroscopic and spectrometric data.
Asunto(s)
Asteraceae/química , Flavonoides/aislamiento & purificación , Lactonas/aislamiento & purificación , Componentes Aéreos de las Plantas/química , Extractos Vegetales/aislamiento & purificación , Sesquiterpenos/aislamiento & purificación , Brasil , Fraccionamiento Químico , Cromatografía , Flavonoides/química , Lactonas/química , Estructura Molecular , Extractos Vegetales/química , Sesquiterpenos/químicaRESUMEN
Two Brazilian species of Dimerostemma (Asteraceae) were chemically investigated. Two known sesquiterpene lactones (STLs), a germacrolide and an eudesmanolide, were isolated from D. episcopale while D. brasilianum afforded the new germacranolide 1beta,5beta/10alpha-trihy-droxy-8alpha-angeloyloxy-germacra-3,11(13)-dien-6alpha,12-olide in addition to a known one. Structure identification based on NMR and MS analyses. 1beta,10alpha,4alpha,5beta-Diepoxy-8alpha-angeloyloxy-costunolide isolated from D. brasilianum was studied for its anti-inflammatory activity. This STL completely inhibited DNA binding of the transcription factor NF-kappaB at a concentration of 5 microm and 10 microM in Jurkat T and Raw 264.7 cells, respectively. Elastase release from human neutrophils was reduced to 50% at a concentrations of 23 microM after stimulation with PAF and of 27 microM after stimulation with fMLP without showing cytotoxic effects. Additionally, elastase was also directly inhibited.
Asunto(s)
Antiinflamatorios no Esteroideos/aislamiento & purificación , Asteraceae/química , Lactonas/aislamiento & purificación , Extractos Vegetales/aislamiento & purificación , Sesquiterpenos/aislamiento & purificación , Animales , Antiinflamatorios no Esteroideos/farmacología , Línea Celular , Granulocitos/efectos de los fármacos , Granulocitos/fisiología , Humanos , Lactonas/farmacología , Macrófagos/citología , Macrófagos/efectos de los fármacos , Ratones , Elastasa Pancreática/sangre , Extractos Vegetales/farmacología , Hojas de la Planta/química , Sesquiterpenos/farmacologíaRESUMEN
The ethyl acetate extract from leaves plus inflorescences of L. salicifolia showed significant trypanocidal activity against trypomastigote forms of T. cruzi, which was due to the flavonoid quercetin-7,3',4'-trimethyl ether and the sesquiterpenoid lychnopholic acid. Despite the use of some species of Lychnophora in folk medicine for pain relief, crude extracts from L. salicifolia Mart. showed no antinociceptive activity in the mouse writhing test.