RESUMEN
Levels of interleukins-6, 8, 10, TNF-alpha and basic fibroblast growth factor (bFGF) were examined in peripheral blood of 60 patients with diabetes mellitus type II and soft tissues infections. It was revealed the elevated levels of proinflammatory (IL-6, 8), anti-inflammatory (IL-10) cytokines and basic fibroblast growth factor at the time of admission. Application of combined ozone therapy including ozonated autohemotherapy and superficial management of wounds with ozone-oxygen mixture resulted in significant decrease of IL-6, 8, 10 production and high level of bFGF on blood serum. Thus effective local bactericidal impact of ozone in combination with normalization of proinflammatory cytokines levels and preserved high level of bFGF in peripheral blood provide better results of wound healing process in patients with diabetes mellitus type II.
Asunto(s)
Transfusión de Sangre Autóloga/métodos , Diabetes Mellitus Tipo 2/complicaciones , Ozono/uso terapéutico , Infecciones de los Tejidos Blandos/terapia , Anciano , Citocinas/sangre , Diabetes Mellitus Tipo 2/sangre , Femenino , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Oxidantes Fotoquímicos/uso terapéutico , Infecciones de los Tejidos Blandos/sangre , Infecciones de los Tejidos Blandos/complicaciones , Resultado del Tratamiento , Cicatrización de HeridasRESUMEN
Clinical results of wound healing dynamics were studied in 60 patients with soft-tissue infection against the background of diabetes mellitus type II. At the same time the study considered indices of intercellular contacts protein tissue expression such as connexin 43 (Cx43) and basic fibroblast growth factor receptors (bFGFR). The basic therapy of biopsy material of wound borders was applied. The reduction of bFGFR expression and the minor growth of Cx43 expression were observed. The pain syndrome proceeded for a long time and there were signs of perifocal inflammation, retard wound healing with granulation tissue. The application of combined method of ozone therapy which included autohemotherapy with ozone and an external management of wound by ozone-oxygen mixture facilitated to considerable shortening of inflammatory phase and regeneration. It was associated with increased Cx43 expression (in 1.9 times) in comparison with initial level and bFGFR was enlarged in 1.7 times to eighth day of postoperative period.
Asunto(s)
Conexina 43/metabolismo , Diabetes Mellitus Tipo 2/complicaciones , Ozono/uso terapéutico , Receptor Tipo 1 de Factor de Crecimiento de Fibroblastos/metabolismo , Enfermedades Cutáneas Infecciosas , Infecciones de los Tejidos Blandos , Procedimientos Quirúrgicos Operativos/métodos , Vías de Administración de Medicamentos , Femenino , Tejido de Granulación/efectos de los fármacos , Tejido de Granulación/metabolismo , Humanos , Inflamación/tratamiento farmacológico , Inflamación/metabolismo , Masculino , Persona de Mediana Edad , Oxidantes Fotoquímicos/uso terapéutico , Atención Perioperativa/métodos , Enfermedades Cutáneas Infecciosas/tratamiento farmacológico , Enfermedades Cutáneas Infecciosas/etiología , Enfermedades Cutáneas Infecciosas/metabolismo , Enfermedades Cutáneas Infecciosas/cirugía , Infecciones de los Tejidos Blandos/tratamiento farmacológico , Infecciones de los Tejidos Blandos/etiología , Infecciones de los Tejidos Blandos/metabolismo , Infecciones de los Tejidos Blandos/cirugía , Resultado del Tratamiento , Cicatrización de Heridas/efectos de los fármacosRESUMEN
The authors have studied alterations occurring both in the ultrastructure of the cell matrix and in the microcirculatory bed of the chronic wound after local exposure to cryoagent. The up-to-date effective methods including laser Doppler flowmetry were used followed by correct statistical processing of the data obtained. The cryogenic stimulation of the wound was shown to result in considerably improved perfusion of the microcirculatory bed, epithelization and remodeling of the scar. It allowed transformation of a chronic process into acute and thus led to considerably accelerated process of regeneration. The developed method of cryogenic treatment of the chronic wound was used in 35 patients, allowed quicker healing of the chronic wounds and made ambulatory treatment of the patients 3 weeks shorter.