RESUMEN
The effect on endoreduplication in sugar beet (Beta vulgaris L.) seedlings of five plant hormones in MS medium, ethylene, 24-epibrassinolide (EBR), gibberellic acid (GA(3) ), kinetin and 1-naphthaleneacetic acid (NAA), as well as a combination of kinetin and NAA at two different concentrations, was studied using flow cytometry. Analyses of DNA content in nuclei of the root, hypocotyl and cotyledons of seedlings growing in vitro were performed during their early development, starting from when the root was 0.5-1.0 cm long until expansion of the first pair of leaves. The proportions of nuclei with different DNA contents were established and the mean C-value calculated. The presence of exogenous plant hormones changed endoreduplication intensity, although to different extents, depending on the organ and developmental stage. Ethylene and NAA stimulated the process, while EBR and kinetin suppressed it and GA did not clearly affect it.
Asunto(s)
Beta vulgaris/crecimiento & desarrollo , Beta vulgaris/metabolismo , Células Cultivadas/metabolismo , Reguladores del Crecimiento de las Plantas/metabolismo , Beta vulgaris/genética , Brasinoesteroides/metabolismo , Ciclo Celular/genética , Cotiledón/crecimiento & desarrollo , Medios de Cultivo/química , Etilenos/metabolismo , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Giberelinas/metabolismo , Hipocótilo/crecimiento & desarrollo , Cinetina/metabolismo , Ácidos Naftalenoacéticos/metabolismo , Técnicas de Amplificación de Ácido Nucleico , Plantones/crecimiento & desarrollo , Esteroides Heterocíclicos/metabolismoRESUMEN
A steam-distilled and an ether extract of onion have both been found to enhance blood fibrinolysis without depressing fibrinogen content in groups of human volunteers. Chemical analysis has shown which sulphur-containing compounds were in highest concentration and common to both and thus likely to carry this property, but their very intense and prolonged flavour precludes their clinical use. One sulphur containing but nearly odourless compound has been identified as having indirect fibrinolytic potential. A species difference in respect of fibrinolysis has been found between English and Spanish onion but is of little importance.