RESUMEN
Thunberg fritillary bulb (the dry bulbs of Fritillaria thunbergii Miq.), a traditional Chinese Medicine, is widely applied as an expectorant and antitussive. In this investigation, the primary metabolites of bulbs, flowers, leaves, and stems of F. thunbergii were analyzed by gas chromatography-mass spectrometry. Principal component analysis, partial least squares-discriminate analysis, orthogonal projection to latent structures-discriminate analysis, and heat map analysis showed that there were dissimilar metabolites, and a negative correlation between amino acids and saccharides in different analytes. Furthermore, carbodiimide, tryptophan, glucose-6-phosphate, xylose, 2-piperidinecarboxylic acid, monoamidomalonic acid, phenylalanine, and histidine were found to play an important role in the plant metabolism net of F. thunbergii.
Asunto(s)
Fritillaria/química , Cromatografía de Gases y Espectrometría de Masas , Metaboloma , Metabolómica , Biología Computacional/métodos , Análisis de Datos , Fritillaria/metabolismo , Redes y Vías Metabólicas , Metabolómica/métodosRESUMEN
OBJECTIVE: To develop a headspace gas chromatography method for the determination of residual organic solvents in Panax notoginseng extracts. METHODS: The samples were injected into HP-INNOWAX capillary column by headspace sampler and analyzed with FID detector using standard addition method. RESULTS: There was a good linearity in the experimental concentration (r=0.9932-0.9999). The rate of recovery was in the rang of 81.74%-111.2%. The numbers of theoretical plates were more than 15000 and the resolutions between the adjacent peaks were more than 2. The RSD of precision and accuracy were both less than 10 %. CONCLUSION: The method is simple,accurate and sensitive with good reproducibility, which can be used for the determination of the residual organic solvents in Panax notoginseng extracts.