Incorporation of fucoxanthin into 3D printed Pickering emulsion gels stabilized by salmon by-product protein/pectin complexes.

The remarkable performance of fucoxanthin (FX) in antioxidant and weight loss applications has generated considerable interest. However, the application of fucoxanthin in the food and pharmaceutical industries is limited due to its highly unsaturated structure. This research aimed to investigate the synergistic mechanism of a unique Pickering emulsion gel stabilized by salmon byproduct protein (SP)-pectin (PE) aggregates and evaluate its ability to enhance the stability and bioavailability of FX. Various analytical techniques, including fluorescence spectroscopy, contact angle testing, turbidity analysis, and cryo-field scanning electron microscopy, were used to demonstrate that electrostatic and hydrophobic interactions between SP and PE contribute to the exceptional stability and wettability of the Pickering emulsion gels. Rheological analysis revealed that increasing the concentration of SP-PEs resulted in shear-thinning behavior, excellent thixotropic recovery performance, higher viscoelasticity, and good thermal stability of the Pickering emulsion gels stabilized by SP-PEs(SEGs). Furthermore, encapsulation of FX in the gels showed protected release under simulated oral and gastric conditions, with the subsequent controlled release in the intestine. Compared to free FX and the control group without PE (SEG-0), SEG-4 exhibited a 1.92-fold and 1.37-fold increase in the total bioavailable fraction of FX, respectively. Notably, during the study, it was observed that SEGs have the potential to serve as cake decoration for 3D printing to replace traditional cream under lower oil phase conditions (50%). These findings suggest that SP-PEs-stabilized Pickering emulsion gels hold promise as carriers for delivering bioactive compounds, offering the potential for various innovative food applications.

Pickering emulsions stabilized by Chlorella pyrenoidosa protein-chitosan complex for lutein encapsulation.

Lutein has many physiological functions like antioxidation, anti-cancer, and anti-inflammation, which presents good potential in the development of functional food for eye protection. However, the hydrophobicity and harsh environment factors during digestive absorption process will greatly reduce lutein bioavailability. In this study, Chlorella pyrenoidosa protein-chitosan complex stabilized Pickering emulsions were prepared, and lutein was encapsulated into corn oil droplets to increase its stability and bioavailability in gastrointestinal digestion. The interaction between Chlorella pyrenoidosa protein (CP) and chitosan (CS), and the effect of chitosan concentration on the emulsifying ability of the complex and emulsion stability were studied. With the increase of CS concentration from 0% to 0.8%, the emulsion droplet size obviously decreased, and the emulsion stability and viscosity increased significantly. In particular, when the concentration was 0.8%, the emulsion system was stable at 80 °C and 400 mM sodium chloride. After ultraviolet irradiation for 48 h, the retention rate of lutein encapsulated in Pickering emulsions was 54.33%, which was significantly higher than that (30.67%) of lutein dissolved in corn oil. The retention rate of lutein in Pickering emulsions stabilized by CP-CS complex was significantly higher than that in Pickering emulsions stabilized by CP only and corn oil after heating at 90 °C for 8 h. The results of simulated gastrointestinal digestion showed that the bioavailability of lutein encapsulated in Pickering emulsions stabilized by CP-CS complex reached 44.83%. These results explored the high-value utilization of Chlorella pyrenoidosa and provided new insights into the preparation of Pickering emulsions and the protection for lutein.