[Acupoints compatibility rules of acupuncture for depression disease based on data mining technology].

Based on data mining technology, the acupoints compatibility rules of acupuncture for depression diseases were explored. The randomized controlled trial (RCT) articles regarding acupuncture for depression diseases published from establishment of database to September 2nd, 2022 were searched in CNKI database, Wangfang database, VIP database, SinoMed database, PubMed, EMbase, Web of Science and Cochrane Library. The use frequency of acupoints, meridian tropism, selection of special acupoints and acupoint association rules for five common depression diseases, including primary depression, post-stroke depression, menopausal syndrome, psychoneurosis and anxiety disorder, were analyzed by Python programming language. Cytoscape software was used to analyze the acupoint association and the disease-acupoint co-occurrence network. As a result, totally 387 articles were included, and 319 acupoints prescriptions for the above five common depression diseases were extracted, involving 159 acupoints. The use frequency of acupoints was 2 574 times in total. The frequently-used acupoints were Baihui (GV 20), Sanyinjiao (SP 6), Taichong (LR 3), Neiguan (PC 6), Shenmen (HT 7), Yintang (GV 24+), Zusanli (ST 36), Hegu (LI 4), Sishencong (EX-HN 1) and Taixi (KI 3), etc. The frequently involved meridians were the governor vessel, foot-taiyang bladder meridian, foot-taiyin spleen meridian, and foot-jueyin liver meridian. The frequency of the special acupoints from high to low was crossing points, five-shu points, yuan-primary points, back-shu points, luo-connecting points, and eight confluent points, etc, which were often used in combination with "Baihui (GV 20)-Yintang (GV 24+)" (the highest degree of association). At the same time, the analysis of the co-occurrence network of depression diseases and acupoints showed that the core acupoints group of acupuncture for depression diseases were Baihui (GV 20), Taichong (LR 3), Shenmen (HT 7), Zusanli (ST 36), Neiguan (PC 6) and Sanyinjiao (SP 6). In conclusion, acupuncture treatment for depression diseases has gradually formed a rule of acupoint compatibility, with special acupoint as the main body and "unblocking the governor vessel, and regulating the spirit and qi " as the main therapeutic principle.

Altering the ratio of palmitic, stearic, and oleic acids in dietary fat affects nutrient digestibility, plasma metabolites, growth performance, carcass, meat quality, and lipid metabolism gene expression of Angus bulls.

This study evaluated the effects of changing the ratio of palmitic, stearic, and oleic acids in dietary fat on nutritional metabolism, growth performance, and meat quality of finishing Angus bulls. Bulls received the following three treatments: (1) a control diet without fat supplement (CON), (2) CON + mixed fatty acid supplement (58% C16:0 + 28% cis-9 C18:1; MIX), (3) CON + saturated fatty acid supplement (87% C16:0 + 10% C18:0; SFA). In summary, both fat treatment diets simultaneously increased saturated fatty acids C16:0 (P = 0.025), C18:0 (P < 0.001) and total monounsaturated fatty acids (P = 0.008) in muscle, thus balancing the ratio of unsaturated to saturated fatty acids in muscle. MIX diet increased the digestibility of dry matter (P = 0.014), crude protein (P = 0.038), and ether extract (P = 0.036). SFA diet increased the daily gain (P = 0.032) and intramuscular fat content (P = 0.043). The high content of C16:0 and C18:0 in the SFA diet promoted weight gain and fat deposition of beef cattle by increasing feed intake, up-regulating the expression of lipid uptake genes and increasing deposition of total fatty acids, resulting in better growth performance and meat quality.

Casein phosphopeptide-calcium chelate: Preparation, calcium holding capacity and simulated digestion in vitro.

In this work, CPP-Ca chelate was synthesized by chelating casein phosphopeptide (CPP) and calcium and characterized by Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) Energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD). The antioxidant activity and calcium holding capacity of CPP-Ca were evaluated and its secondary structure transition was monitored during gastrointestinal digestion by in situ Raman spectroscopy. The results demonstrated that calcium chelating rate reached 40 % and calcium ion was bound to CPP mainly through the interaction of carboxyl and amino groups. The result of calcium holding capacity confirmed the formation of calcium phosphate precipitates could be delayed by 10-15 min with increasing CPP concentration. In vitro simulated digestion revealed CPP-Ca exhibited excellent calcium solubility and its secondary structural changes occurred, especially α-helix and ß-sheet content. These findings provided significant insights into enhancing bioavailability of calcium supplements and developing of calcium functional foods for human and animals.

Calcium Activation of Parvalbumin Neurons Induced by Electrical Motor Cortex Stimulation.

Electrical motor cortex stimulation (EMCS) has been used for Parkinson's Disease (PD) treatment. Some studies found that distinct cell types might lead to selective effects. As the largest subgroup of interneurons, Parvalbumin (PV) neurons have been reported to be involved in the mechanisms of therapeutic efficacy for PD treatment. However, little is known about their responses to the EMCS. In this study, we used in-vivo two-photon imaging to record calcium activities of PV neurons (specific type) and all neurons (non-specific type) in layer 2/3 primary motor cortex (MI) during EMCS with various stimulus parameters. We found PV neurons displayed different profiles of activation property compared to all neurons. The cathodal polarity preference of PV neurons decreased at a high-frequency stimulus. The calcium transients of PV neurons generated by EMCS trended to be with large amplitude and short active duration. The optimal activation frequency of PV neurons is higher than that of all neurons. These results improved our understanding of the selective effects of EMCS on specific cell types, which could bring more effective stimulation protocols for PD treatment.

Online Decoding System with Calcium Image From Mice Primary Motor Cortex.

With the development of calcium imaging, neuroscientists have been able to study neural activity with a higher spatial resolution. However, the real-time processing of calcium imaging is still a big challenge for future experiments and applications. Most neuroscientists have to process their imaging data offline due to the time-consuming of most existing calcium imaging analysis methods. We proposed a novel online neural signal processing framework for calcium imaging and established an Optical Brain-Computer Interface System (OBCIs) for decoding neural signals in real-time. We tested and evaluated this system by classifying the calcium signals obtained from the primary motor cortex of mice when the mice were performing a lever-pressing task. The performance of our online system could achieve above 80% in the average decoding accuracy. Our preliminary results show that the online neural processing framework could be applied to future closed-loop OBCIs studies.

[Change of root morphology in intercropping systems of wheat and faba bean under different phosphorus levels and its relationship with endogenous hormones]. / 不同磷水平下小麦-èš•è±†é—´ä½œæ ¹ç³»å½¢æ€çš„å˜åŒ–åŠå ¶ä¸Žå† æºæ¿€ç´ çš„å ³ç³».

We examined the correlation between changes of root morphology and endogenous hormones in intercropping systems of wheat and faba bean under different phosphorus levels by hydroponics. Compared with monocropping wheat (MW), the intercropping of wheat and faba bean (W∥F) significantly increased root length of wheat, reduced root average diameter of wheat, and increased root surface area under the condition of 1/2P (low P) level. At the conventional phosphorus level, intercropping significantly reduced root average diameter of wheat, and increased root length and root surface area. Compared with monocropping faba bean (MF), W∥F significantly promoted the growth of faba bean root and increased root surface area of faba bean. At the level of 1/2P, intercropping significantly increased the content of auxin (IAA), abscisic acid (ABA), sali-cylic acid (SA) and jasmonic acid (JA). At the conventional phosphorus level, intercropping could significantly increase the content of IAA, ABA and JA in wheat root, while no significant difference in the SA content of wheat root between monocropping and intercropping wheat was found. Intercropping could increase the content of ABA and SA in faba bean roots, but did not affect IAA and JA contents of faba bean roots. There was no significant correlation between the contents of endogenous hormones (IAA, ABA, SA and JA) and root morphology (root length, root average diameter and root surface area) of wheat and faba bean roots in wheat or faba bean monocropping system. In wheat and faba bean intercropping system, there was a positive correlation between IAA contents of wheat and faba bean and their root length and root surface area. W∥F enhanced IAA of wheat and faba bean root, which was an important factor driving the change of root morphology in the intercropping system of wheat and faba bean.

Identification of 2-PMPA as a novel inhibitor of cytosolic carboxypeptidases.

Cytosolic carboxypeptidases (CCPs) comprise a unique subfamily of M14 carboxypeptidases and are erasers of the reversible protein posttranslational modification- polyglutamylation. Potent inhibitors for CCPs may serve as leading compounds targeting imbalanced polyglutamylation. However, no efficient CCP inhibitor has yet been reported. Here, we showed that 2-phosphonomethylpentanedioic acid (2-PMPA), a potent inhibitor of the distant M28 family member glutamate carboxypeptidase II (GCPII), rather than the typical M14 inhibitor 2-benzylsuccinic acid, could efficiently inhibit CCP activities. 2-PMPA inhibited the recombinant Nna1 (a.k.a. CCP1) for hydrolyzing a synthetic peptide in a mixed manner, with Ki and Ki' being 0.11 µM and 0.24 µM respectively. It inhibited Nna1 for deglutamylating tubulin, the best-known polyglutamylated protein, with an IC50 of 0.21 mM. Homology modeling predicted that the R-form of 2-PMPA is more favorable to bind Nna1, unlike that GCPII prefers to S-form. This work for the first time identified a potent inhibitor for CCP family.

Screening bioactive components of Glycyrrhiza uralensis Fisch. with isolated perfused lung extraction and HPLC-ESI-MSn analysis.

The isolated perfused rat lung (IPL), coupled with high performance liquid chromatography\tandem mass spectrometry analysis (HPLC-ESI-MSn), has been developed as a tool for screening bioactive components in Glycyrrhiza uralensis Fisch. (GU). First, IPL was perfused with the water extract of GU (EGU), the bioactive components in the EGU would selectively combine to the receptors or channels of lung. By changing the pH of perfused solution, the combined components were eluated and then detected by HPLC-ESI-MSn. Four compounds were detected in the desorption eluate of IPL, among these compounds, liquiritin (1), ononin (2) and glycyrrhizic acid (4) were identified by comparing with the chromatography of the standards, while licorice-saponin G2 (3) were determined by analysis of the structure clearage characterization of mass spectrometry. Then, due to the lack of compound 3 sample, compounds 1, 2 and 4 with respective concentrations of 50 µM, 5 µM, 500 nM, 50 nM and 5 nM were applied to evaluate the protective effect of pulmonary epithelial cells (PEC, A549 cell) injury induced by lipopolysaccharide (LPS) for anti-inflammatory activity assessment. The results showed that except the 5 nM group of compound 1, 5 nM and 50 nM groups of compound 2, all other groups could remarkably inhibit the PEC injury (vs LPS group, 2-500 nM groups: p < 0.05; other groups: p < 0.01), all compound showed the dose-dependent effect. In conclusion, IPL coupled with HPLC-ESI-MSn was successfully used to screen the anti-inflammatory components of GU for the first time. The application of IPL coupled with HPLC-ESI-MSn for screening bioactive components of TCMs is rapid, convenient and reliable, and the isolated perfused technology could be extended to isolated heart, liver, kidney, and so on.

Decoding with Calcium Signals from Layer 2/3 Motor Cortex during A Pressing Movement.

Brain-machine interfaces (BMIs) have been promising for not only neuroprosthesis research but also brain function investigation. Electrophysiological recording commonly used in traditional BMIs is spatially sparse and lack of information about neuron types and spatial organization. However, optical imaging methods might avoid these limitations by providing dense, spatially organized and annotated with genetic information over a large field of view. Here, we tried to demonstrate the potential of calcium imaging signals obtained through the one-photon microscope in neural decoding. When mice were trained to perform a lever press task to obtain water as rewards, the calcium signals of neurons in their layer 2/3 motor cortex were recorded by microscope. With the calcium signals, we analyzed the neural activity at both single individual neuron and neuronal population level. We found two typical classes of pressing-related neurons and distinct ensemble activity patterns between a pressing movement and baseline. The decoding results further demonstrated that the movement-related information could be more completely specified by population response structure. Our results suggested that neural signals from more types and a larger amount of neurons, are crucial for accurate decoding in BMI applications.

Anti-angiogenic activity of para-coumaric acid methyl ester on HUVECs in vitro and zebrafish in vivo.

BACKGROUND: Para-coumaric acid methyl ester (pCAME) is one of the bioactive components of Costus speciosus (Koen) Sm. (Zingiberaceae). This plant is traditionally used in Asia to treat catarrhal fevers, worms, dyspepsia, and skin diseases. PURPOSE: To investigate the anti-angiogenic activity of pCAME and its molecular mechanism of action. STUDY DESIGN: We investigated the anti-angiogenic activity of pCAME on human umbilical vein endothelial cells (HUVECs) in vitro and zebrafish (Danio rerio) in vivo. METHODS: In vitro cell proliferation, would healing, migration and tube formation assays were used, along with in vivo physiological angiogenic vessel formation, tumor-induced angiogenic vessel formation assays on zebrafish model. qRT-PCR and RNA-seq were also used for the target investigation. RESULTS: pCAME could inhibit the proliferation, would healing, migration and tube formation of HUVECs, disrupt the physiological formation of intersegmental vessels (ISVs) and the subintestinal vessels (SIVs) of zebrafish embryos, and inhibit tumor angiogenesis in the zebrafish cell-line derived xenograft (zCDX) model of SGC-7901 in a dose-dependent manner. Mechanistic studies revealed that pCAME inhibited vegf/vegfr2 and ang/tie signaling pathways in zebrafish by quantitative RT-PCR analysis, and regulated multi-signaling pathways involving immune, inflammation and angiogenesis in SGC-7901 zCDX model by RNA-seq analysis. CONCLUSION: pCAME may be a multi-target anti-angiogenic drug candidate and hold great potential for developing novel therapeutic strategy for cancer treatment.

A novel tricarbonylmethane agent (CMC2.24) reduces human pancreatic tumor growth in mice by targeting Ras.

Pancreatic Cancer (PC) is a deadly disease in need of new therapeutic options. We recently developed a novel tricarbonylmethane agent (CMC2.24) as a therapeutic agent for PC, and evaluated its efficacy in preclinical models of PC. CMC2.24 inhibited the growth of various human PC cell lines in a concentration and time-dependent manner. Normal human pancreatic epithelial cells were resistant to CMC2.24, indicating selectivity. CMC2.24 reduced the growth of subcutaneous and orthotopic PC xenografts in mice by up to 65% (P < 0.02), and the growth of a human patient-derived tumor xenograft by 47.5% (P < 0.03 vs vehicle control). Mechanistically, CMC2.24 inhibited the Ras-RAF-MEK-ERK pathway. Based on Ras Pull-Down Assays, CMC2.24 inhibited Ras-GTP, the active form of Ras, in MIA PaCa-2 cells and in pancreatic acinar explants isolated from Kras mutant mice, by 90.3% and 89.1%, respectively (P < 0.01, for both). The inhibition of active Ras led to an inhibition of c-RAF, MEK, and ERK phosphorylation by 93%, 91%, and 87%, respectively (P < 0.02, for all) in PC xenografts. Furthermore, c-RAF overexpression partially rescued MIA PaCa-2 cells from the cell growth inhibition by CMC2.24. In addition, downstream of ERK, CMC2.24 inhibited STAT3 phosphorylation levels at the serine 727 residue, enhanced the levels of superoxide anion in mitochondria, and induced intrinsic apoptosis as shown by the release of cytochrome c from the mitochondria to the cytosol and the further cleavage of caspase 9 in PC cells. In conclusion, CMC2.24, a potential Ras inhibitor, is an efficacious agent for PC treatment in preclinical models, deserving further evaluation.

Baicalin potentiates TRAIL­induced apoptosis through p38 MAPK activation and intracellular reactive oxygen species production.

The combination of tumor necrosis factor­related apoptosis­inducing ligand (TRAIL) with other agents has been recognized as a promising strategy to overcome TRAIL resistance in cancer cells. Baicalin (5, 6­dihydroxy­7­o­glucuronide flavone) is a flavonoid from the root of the medicinal herb Scutellaria baicalensis Georgi, which has been reported to exert antioxidant, anti­inflammatory, antiviral and anticancer activities in vitro. However, the effect of baicalin on TRAIL­induced cytotoxicity has not been previously reported. In the present study, the effect of combining TRAIL and baicalin was investigated in non­small cell lung cancer cell lines. The results revealed that baicalin was able to sensitize A549 and H2009 cells to TRAIL­induced apoptosis. This was detected by the potentiation of poly­adenosine­5'­diphosphate­ribose polymerase cleavage and Annexin V­fluorescein isothiocyanate staining of cells co­treated with baicalin and TRAIL. In addition, p38 mitogen­activated protein kinase was activated in baicalin and TRAIL co­treated cancer cells, whereas the p38 inhibitor SB203580 effectively suppressed cell death within the co­treated cells. Butylated hydroxyanisole and N­acetyl­cysteine, known reactive oxygen species (ROS) scavengers, significantly suppressed the potentiated cytotoxicity induced by baicalin and TRAIL co­treatment. The present study is the first, to the best of our knowledge, to demonstrate that baicalin enhances the anticancer activity of TRAIL via p38 activation and ROS accumulation, and may be exploited for anticancer therapy.

Effects of energy expenditure and Ucp1 on photoperiod-induced weight gain in collard lemmings.

OBJECTIVE: To determine the role of total energy expenditure (TEE) and its components in the ability of collared lemmings to increase weight in response to a decrease in photoperiod. RESEARCH METHODS AND PROCEDURES: Energy expenditure was measured by 24-hour indirect calorimetry concurrent with food-intake studies. TEE and resting and nonresting energy expenditure (REE and NREE, respectively) were adjusted for body weight by analysis of covariance (ANCOVA). Uncoupling protein 1 (Ucp1) mRNA levels from interscapular brown adipose tissue were determined by Northern blot. RESULTS: TEE and REE of lemmings exposed to a short photoperiod for 10 days were significantly lower than that of lemmings exposed to a long photoperiod (p < 0.05), whereas NREE was not significantly different (p = 0.44). Ucp1 mRNA levels in interscapular brown adipose tissue were 50% lower in short- vs. long-photoperiod lemmings (p < 0.01). Ucp1 mRNA levels were positively related to REE (r2 = 0.79, p < 0.01). After adjustment of REE for differences in Ucp1 mRNA levels, there was no longer a significant difference attributable to photoperiod treatment (p = 0.54). DISCUSSION: The results of this study indicate that the increase in body mass that occurs when collared lemmings are exposed to a short photoperiod may be primarily fueled by a decrease in REE and is correlated with a decrease in Ucp1 mRNA levels.